Assessment of Cellular Responses after Short- and Long-Term Exposure to Silver Nanoparticles in Human Neuroblastoma (SH-SY5Y) and Astrocytoma (D384) Cells
Figure 2
Data comparisons between AgNPs and AgNO3 in SH-SY5Y, D384 and A549 cell lines. Panels (a), (b), and (c) show mitochondrial function evaluation by MTT assay after 4 h, 24 h and 48 h exposure to 1 and 10 μg/ml of AgNPs and AgNO3. *Different from control in each cell line , statistical analysis by ANOVA followed by Tukey’s test. Error bars indicate S.D. Panels (d), (e), and (f) show membrane integrity by calcein-AM/Propidium Iodide staining after 4 h, 24 h and 48 h exposure to 1 and 10 μg/ml of AgNPs and AgNO3; scale bar 100 μm. AgNO3 produced a more pronounced cytotoxic effect to that caused by comparable amount of AgNPs on cerebral cell lines. In A549 cells, similar low cytotoxic profile was observed after both treatment types (AgNPs and AgNO3).
