Assessment of Cellular Responses after Short- and Long-Term Exposure to Silver Nanoparticles in Human Neuroblastoma (SH-SY5Y) and Astrocytoma (D384) Cells

Table 1

EC₅₀ after 4, 24, and 48 h exposure to AgNPs (1–100 μg/mL). Effective concentrations causing 50% (EC₅₀ values, 𝜇g/mL) loss of cell viability (evaluated by MTT test) for SH-SY5Y, D384, and A549 cell lines following exposure to increasing concentrations of AgNP (0–100 μg/mL).

Cell type	EC50 (𝜇g/mL)
	4 h; exposure	24 h; exposure	48 h; exposure
SH-SY5Y	40.71±2.00	30.73±3.20	28.38±3.00
D384	49.49±2.10	33.41±4.00	30.21±3.40
A549	No cytotoxic effects	No cytotoxic effects	Cytotoxic effects starting at >100 𝜇g/mL (about 65% viability)