Optimization of Culture Conditions (Sucrose, pH, and Photoperiod) for In Vitro Regeneration and Early Detection of Somaclonal Variation in Ginger Lime (Citrus assamensis)
Figure 2
Scanning electron micrographs (SEM) of in vivo and in vitro grown Citrus assamensis leaves. (a) Abaxial surface of in vitro and (b) in vivo grown leaf showing anomocytic stomata; (c) abaxial surface of in vitro grown leaf, showing vivid anomocytic stomata in between lined epidermal cells; (d-e) abaxial surface of in vivo grown leaf showing clearly defined anomocytic stomata that appeared from the epidermal surface; (f) stoma of in vitro grown leaf sunk into epidermal surface; (g-h) abaxial surface of in vivo grown leaf, showing the presence of oil glands; (i) abaxial surface of in vitro grown leaf showing the absence of oil gland; (j-k) lack of trichomes was observed from primary veins of in vivo grown leaf; (l) primary vein of in vitro grown leaf showing the presence of trichome.
