Optimization of Culture Conditions (Sucrose, pH, and Photoperiod) for In Vitro Regeneration and Early Detection of Somaclonal Variation in Ginger Lime (Citrus assamensis)
Figure 3
Histological examinations on in vivo and in vitro grown Citrus assamensis leaves. (a–c) Cross sections of leaf base of (a-b) in vivo and (c) in vitro plants, showing the presence of (1) vascular system, (2) cell structures, (3) cuticle, (4) palisade layer, (5) oil gland, (6) drusa, (7) trichome, and (8) lateral vascular bundle. (d–f) Cross sections of leaf midrib of (d-e) in vivo and (f) in vitro plants, showing the presence of (1) vascular system, (2) cell structures, (3) cuticle, (4) palisade layer, (5) oil gland, (6) drusa, (7) trichome, and (8) lateral vascular bundle. (g–i) Cross sections of leaf tip (apex) of (g-h) in vivo and (i) in vitro plants, showing the presence of (1) vascular system, (2) cell structures, (3) palisade layer, (4) oil gland, and (5) drusa.
