Optimization of Culture Conditions (Sucrose, pH, and Photoperiod) for In Vitro Regeneration and Early Detection of Somaclonal Variation in Ginger Lime (Citrus assamensis)
Table 2
The effects of different combinations and concentrations of BAP and NAA on root explants of Citrus hybrids, cultured on solid MS media at °C. Cultures were maintained at 16 hours of light and 8 hours of darkness, with 1000 lux intensity of light.
MS + hormone
Observations
Callus (%)
Organogenesis
BAP (mgL−1)
NAA (mgL−1)
Shoot (%)
Root (%)
0.0
1.0
White callus formation after 35 days Root formation after 35 days
NR
0.0
2.0
White callus formation after 35 days Root formation after 35 days
NR
1.0
0.0
Shoot formation after 60 days
NR
NR
2.0
0.0
Shoot formation after 60 days
NR
NR
0.5
0.5
Yellowish callus formation after 30 days
NR
NR
1.0
0.5
Greenish callus formation after 30 days
NR
NR
1.5
0.5
Yellowish callus formation after 18 days
NR
NR
2.0
0.5
Brown callus formation after 25 days
NR
NR
0.5
1.0
Yellowish callus formation after 30 days
NR
NR
1.0
1.0
Yellowish callus formation after 22 days
NR
NR
1.5
1.0
Yellowish callus formation after 25 days
NR
NR
2.0
1.0
Yellow callus formation after 20 days
NR
NR
0.5
1.5
Green callus formation after 25 days
NR
NR
1.0
1.5
Brown callus formation after 25 days Shoot formation after 55 days
NR
1.5
1.5
Yellow callus formation after 25 days
NR
NR
2.0
1.5
Yellow callus formation after 30 days Root formation after 50 days
NR
0.5
2.0
Yellow callus formation after 16 days
NR
NR
1.0
2.0
Greenish callus formation after 18 days Shoot formation after 55 days
NR
1.5
2.0
Yellowish callus formation after 18 days
NR
NR
2.0
2.0
Yellowish callus formation after 16 days
NR
NR
3.0
3.0
Yellow callus formation after 30 days
NR
NR
Mean SE, . Mean values with different letters in the same column are significantly different at (NR: no response).
