Research Article

Optimization of Culture Conditions (Sucrose, pH, and Photoperiod) for In Vitro Regeneration and Early Detection of Somaclonal Variation in Ginger Lime (Citrus assamensis)

Table 4

The effects of different concentrations of sucrose on stem, root, and leaf explants, cultured on optimum media (MS + 1.5 mgL−1 NAA + 2.0 mgL−1 BAP) at °C. Cultures were maintained at 16 hours of light and 8 hours of darkness, with 1000 lux intensity of light.

Concentrations of sucrose (gL−1)ExplantsObservationsCallus (%)Organogenesis
Shoot (%)Root (%)

20StemShoots formation after 60 daysNR NR
RootGreen callus formation after 50 days NRNR
LeafNo responseNRNRNR

30StemGreen callus formation after 25 days
Shoots formation after 30 days
NR
RootYellow callus formation after 30 days NRNR
LeafNo responseNRNRNR

40StemShoots formation after 50 daysNR NR
RootGreen callus formation after 40 days NRNR
LeafNo responseNRNRNR

50StemGreen callus formation after 40 days
Shoots formation after 50 days
NR
RootYellow callus formation after 40 days NRNR
LeafNo responseNRNRNR

Mean SE, . Mean values with different letters (subject to different explant type) in the same column are significantly different at (NR: no response).