Optimization of Culture Conditions (Sucrose, pH, and Photoperiod) for In Vitro Regeneration and Early Detection of Somaclonal Variation in Ginger Lime (Citrus assamensis)
Table 4
The effects of different concentrations of sucrose on stem, root, and leaf explants, cultured on optimum media (MS + 1.5 mgL−1 NAA + 2.0 mgL−1 BAP) at °C. Cultures were maintained at 16 hours of light and 8 hours of darkness, with 1000 lux intensity of light.
Concentrations of sucrose (gL−1)
Explants
Observations
Callus (%)
Organogenesis
Shoot (%)
Root (%)
20
Stem
Shoots formation after 60 days
NR
NR
Root
Green callus formation after 50 days
NR
NR
Leaf
No response
NR
NR
NR
30
Stem
Green callus formation after 25 days Shoots formation after 30 days
NR
Root
Yellow callus formation after 30 days
NR
NR
Leaf
No response
NR
NR
NR
40
Stem
Shoots formation after 50 days
NR
NR
Root
Green callus formation after 40 days
NR
NR
Leaf
No response
NR
NR
NR
50
Stem
Green callus formation after 40 days Shoots formation after 50 days
NR
Root
Yellow callus formation after 40 days
NR
NR
Leaf
No response
NR
NR
NR
Mean SE, . Mean values with different letters (subject to different explant type) in the same column are significantly different at (NR: no response).