Solid Tumor-Targeting Theranostic Polymer Nanoparticle in Nuclear Medicinal Fields

Figure 6

Effect of the first Lactosome dose on the Lactosome ABC phenomenon. NIRF images of mice at (a) 15 min (view from A in Figure 6(d)) and (b) 24 h (view from C in Figure 6(d)) after ICG-Lactosome of 2nd dose. Lactosomes (5, 25, and 50 mg/kg/100 L and 150, 250, and 350 mg/kg/200 L) were injected into the mice 7 days before the ICG-Lactosome administration. SUIT-2/pEF/luc cells were transplanted at the right femoral region of mice, in which tumor sites are indicated by white arrows. The fluorescein signal ranges were set to be the same for all the images from max count 5000 to min count 1000. (c) Time schedule for the NIRF imaging. Black and green arrows indicate the injection time points of Lactosome and ICG-Lactosome, respectively. NIRF imaging was performed at red arrows. (d) NIRF images were taken using Shimadzu Clairvivo OPT, which can take five images from different directions (A–E) with one time shot. The white circles indicate positions of ROI (L: liver; B: background). Reprinted from Biochemica et Biophysica Acta (BBA)-General Subjects, 2013, 1830, 4046–4052., E. Hara, A. Makino, K. Kurihara, M. Sugai, A. Shimizu, I. Hara, E. Ozeki, and S. Kimura. “Evasion from accelerated blood clearance of nanocarrier named as “Lactosome” induced by excessive administration of Lactosome.” Copyright (2013), with permission from Elsevier B.V.

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