Molecular Characterization of Putative Virulence Determinants in Burkholderia pseudomallei

Table 1

Bacterial strains and plasmids used.

Strains or plasmids	Relevant characteristic(s)	Reference
Strains
B. pseudomallei
Bp-CMS	Clinical isolate from blood; wild type parental strain for generation of insertion mutants	Current study
BPSL2033::Km	BPSL2033::Km derivative of CMS; Kan^R
BPSL2033::Km (pC-BPSL2033)	BPSL2033::Km complemented with pC-BPSL2033 plasmid; Kan^R Cm^R
BP1026B_I2784::Km	BP1026B_I2784::Km derivative of CMS; Kan^R
BP1026B_I2784::Km (pC-BP1026B_I2784)	BP1026B_I2784::Km complemented with pC-BP1026B_I2784 plasmid; Kan^R Cm^R
BP1026B_I2780::Km	BP1026B_I2780::Km derivative of CMS; Kan^R
BP1026B_I2780::Km (pC-BP1026B_I2780)	BP1026B_I2780::Km complemented with pC-BP1026B_I2780 plasmid; Kan^R Cm^R
BURPS1106A_A0094::Km	BURPS1106A_A0094::Km derivative of CMS; Kan^R
BURPS1106A_A0094::Km   (pC-BURPS1106A_A0094)	BURPS1106A_A0094 ::Km complemented with pC-BURPS1106A_A0094 plasmid; Kan^R Cm^R
BURPS1106A_1131::Km	BURPS 1106A_1131::Km derivative of CMS; Kan^R
BURPS1106A_1131::Km   (pC-BURPS1106A_1131)	BURPS 1106A_1131::Km complemented with pC-BURPS1106A_1131 plasmid; Kan^R Cm^R
BURPS1710A_1419::Km	BURPS1710A_1419::Km derivative of CMS; Kan^R
BURPS1710A_1419::Km   (pC-BURPS1710A_1419)	BURPS1710A_1419::Km complemented in trans with pC-BURPS1710A_1419 plasmid; Kan^R Cm^R
BPSL3147::Km	BPSL3147::Km derivative of CMS; Kan^R
BPSL3147::Km (pC-BPSL3147)	BPSL3147::Km complemented with pC-BPSL3147 plasmid; Kan^R Cm^R
E. coli
DH10B	F_ mcrA (mrr-hsdRMS-mcrBC)80lacZ M15 lacX74 recA1 endA1 araD 139 Δ(ara, leu)7697 galU galK ^-rpsL nupG	Prof. Dr. Jin-Town Wang
CC118pir	Δ(ara-leu) araD Δ lacX74 galE galK phoA20 thi-1 rpsE rpoB argE(Am) recA1 pir phage lysogen
S17-1pir	hsdR recA pro RP4-2 (Tc::Mu; Km::Tn7) (pir)
OP50-1	A streptomycin-resistant derivative of E.coli OP50 used as food source in C. elegans cultivation and uninfected control	Kind gifts from Prof. Dr. Sheila Nathan
Plasmid
pUT-Km	pUT-Km1 derived plasmid, with miniTn5 excised by EcoRI, tnp excised by SalI, and bla removed by ApaLI and then with an insertion of Km resistance cassette from pUC4K into PstI site; oriR6K mobRP4 Kan^R Amp^R	Chuang et al., 2006 [15]
pGEM-T easy	Cloning vector for PCR cloning; Amp^R	Promega, USA