Molecular Characterization of Putative Virulence Determinants in Burkholderia pseudomallei

Table 2

Primers used for PCR and construction of mutants and complemented plasmids.

Gene	Primer name	Nucleotide sequence (5′ to 3′)	Purpose
BPSL2033	2033IF	AGAACTTCGAGCAATTGCTG	Internal PCR
	2033IR	GAGAGATGACGTTCGGTCTT	Internal PCR
	M2033F	GTGAACTGGTACAAAGAAATATCG	Mutant confirmation
	M2033R	CACGTTTCTCGGATAGAGC	Mutant confirmation
	C2033F	CTAGAGCGCGGCCTCGCG	Complementation of BPSL2033::Km
	C2033R	CATCACTCGGCGCAATGAGACTG	Complementation of BPSL2033::Km
BP1026B_I2784	2784IF	GTCGAGAGTACGGTGTGTTC	Internal PCR
	2784IR	CCTGCGAAATCCTTATCAC	Internal PCR
	M2784F	CTGTTTTCTAAGCGTCAGAAG	Mutant confirmation
	M2784R	AAATATGCAGGAAATAGCCCG	Mutant confirmation
	C2784F	CCTTCGCGCTGATTTGGT	Complementation of BP1026B_I2784::Km
	C2784R	CTACTTCGTAGCTTGATGCGCC	Complementation of BP1026B_I2784::Km
BP1026B_I2780	2780IF	AAACCAGAAGGGCGATTTC	Internal PCR
	2780IR	GCGTTCTTTTAAGAATTGGGTAG	Internal PCR
	M2780F	CAGGTACGATTCATGGAACG	Mutant confirmation
	M2780R	GGTATTCCGTGACCTGAATGT	Mutant confirmation
	C2780F	AGGCTCGGAGTAGTAACACTT	Complementation of BP1026B_I2780::Km
	C2780R	CTACTGCCTATGCTGGGGTAT	Complementation of BP1026B_I2780::Km
BURPS1106A_A0094	94IF	AGTCGGGGGGTACACCTAC	Internal PCR
	94IR	CGACACCGAGGAAAATTTC	Internal PCR
	M94F	GTGAATGTCGATCTTGCG	Mutant confirmation
	M94R	TCAATCTCCAGCGAGCTT	Mutant confirmation
	C94F	TTCTACCAGCGACTTGGC	Complementation of BURPS1106A_A0094::Km
	C94R	TCAATCTCCAGCGAGCTT	Complementation of BURPS1106A_A0094::Km
BURPS1106A_1131	1131IF	AAGGAGTTGGGTACGTCG	Internal PCR
	1131R	CCCTTGTGCCATTGATAG	Internal PCR
	pUT-R	TTTGAGTGACACAGGAACAC	Mutant confirmation
	C1131F	GTGGTTCAGCCAGGCACG	Complementation of BURPS1106A_1131::Km
	C1131R	GTATGTGTCGTCCGCATTTG	Complementation of BURPS1106A_1131::Km
BURPS1710A_1419	1419IF	GCGATAGCGATTGGAAAACT	Internal PCR
	1419IR	GAATCCAGACCCATTCCGT	Internal PCR
	KmF	ATGAGCCATATTCAACGGGA	Mutant confirmation
	C1419F	TGCACAAGCTGTTCAAATG	Complementation of BURPS1710A_1419::Km
	C1419R	TTAAGGCTTGGGTGCAAG	Complementation of BURPS1710A_1419::Km
BPSL3147	3147IF	GACCAGTACGCGCTCAAG	Internal PCR
	3147IR	GAACGAATACTTGTCGATCG	Internal PCR
	M3147F	GATGTACACGTTCAACGACAAG	Mutant confirmation
	M3147R	CTCTTCCGGCATCTCGTA	Mutant confirmation
	C3147F	TTCAGGGTTACGAAGCGAAG	Complementation of BPSL3147::Km
	C3147R	TCAGTGCAGCCGGATGCTCG	Complementation of BPSL3147::Km