Outer Membrane Proteome Analysis of Indian Strain of Pasteurella multocida Serotype B:2 by MALDI-TOF/MS Analysis

Figure 1

Coomassie blue-stained SDS-PAGE showing outer membrane protein profile of Pasteurella multocida serotype B:2 (P52) strain cultured in iron-rich or iron-limited media. Lanes 1, 2: OMPs growing in iron limiting condition, lane M: prestained protein ladder, and lanes 3, 4: OMPs growing in iron-rich condition. IR: bacteria grown under iron-regulated condition; N: bacteria grown under normal condition (iron-rich condition). IR-1 to IR-8 and N1 to N8 represent the portion of the gel slice cut and sent for MALDI-TOF/MS analysis. The proteins identified in the MASCOT analysis are presented in Table 1.