The Scientific World Journal

Research Article

Outer Membrane Proteome Analysis of Indian Strain of Pasteurella multocida Serotype B:2 by MALDI-TOF/MS Analysis

Table 1

Outer membrane proteins of Pasteurella multocida serotype B:2 identified by Mascot and BLASTP analysis.


Band ID^a	MASCOT search homology with species and NCBI accession number	Molecular weight (Da)	Isoelectric point	Sequence coverage (%)	MASCOT scores

N1 ~90 kDa	Transferrin binding protein A [Pasteurella multocida], Acc. number CAD90055	87808.22	9.71	58.12%	332
	TonB-dependent lactoferrin and transferrin receptor [Pasteurella multocida subsp. multocida str. P52VAC], Acc. number ZP_15715832	87808.22	9.64	58.12%	338
	omp87 protein [Pasteurella multocida] Acc. number CAD20126	87688.32	5.99	50.63%	181
	Protective surface antigen D15/OMP assembly factor Yae⁺ precursor Acc. number YP_005177207	87800	6.58	52.47%	178
	Hypothetical protein KCU_10206 [Pasteurella multocida subsp. multocida str. P52VAC] Acc. number ZP_15715705	87938.5	6.58	53.86%	185
	hmbR Acc. number CAD58169	77400	9.75	41.26%	207

N2 ~75 kDa	TonB-dependent lactoferrin and transferrin receptor [Pasteurella multocida subsp. multocida str. P52VAC] Acc. number ZP_15715832	87808.22	9.64	34.69%	70.3
N2 ~75 kDa	Transferrin binding protein A [Pasteurella multocida] Acc. number CAD90055	89284.06	9.71	33.08%	68.8

N3 ~50 kDa	Outer membrane protein A [Pasteurella multocida] Acc. number ADF95751	38433.84	9.54	78.21%	593
	Membrane protein, aromatic hydrocarbon degradation family Acc. number YP_005176018	46700	9.44	55.73%	321
	Long-chain fatty acid transport protein [Pasteurella multocida subsp. gallicida P1059] Acc. number ZP_18469055	47545.25	9.44	57.79%	331
	pm1069 protein [Pasteurella multocida] Acc. number ACT85941	47515.12	9.3	50.80%	314

N4 ~45 kDa	47 kDa outer membrane protein [Pasteurella multocida subsp. multocida str. 3480] Acc. number YP_006239073	47270.14	9.34	33.26%	97.7
	Hypothetical protein AAUPMB_11295, partial [Pasteurella multocida subsp. multocida str. Anand1_buffalo] Acc. number ZP_15708593	18875.39	8.69	62.50%	175
	Long-chain fatty acid transport protein [Pasteurella multocida subsp. gallicida P1059] Acc. number EJZ79884	47545.25	9.44	32.96%	97.4
	Pm 1069 protein (OMP P1 precursor) Acc. number ACT85941	47500	9.3	26.88%	90
	Glycerophosphodiester phosphodiesterase [Pasteurella multocida] Acc. number WP_005752028	41223	6.57	34%	82

N5 ~40 kDa	Outer membrane protein A [Pasteurella multocida] Acc. number ADF95751	38433.84	9.58	78.21%	593
	Hypothetical protein PM0786 [Pasteurella multocida subsp. multocida str. Pm70] Acc. number NP_245723	38121.61	9.44	61.19%	409
	Hypothetical protein AAUPMB_08244, partial [Pasteurella multocida subsp. multocida str. Anand1_buffalo]	20826	N.A.	N.A.	103

N6 ~33 kDa	Outer membrane protein H [Pasteurella multocida] Acc. number EJZ80794	35652.51	9.75	43.24%	250
N6 ~33 kDa	39 kDa adhesive protein [Pasteurella multocida] Acc. number ABR27206	35248.11	9.14	60.80%	434

N7 ~30 kDa	Outer membrane protein H [Pasteurella multocida] Acc. number EJZ80794	35652.51	9.75	51.65%	232
	Adhesive protein [Pasteurella multocida] Acc. number ABX58059	33740.43	9.58	37.06%	243
	39 kDa adhesive protein [Pasteurella multocida] Acc. number ABR27206	35248.11	9.14	60.80%	434

N8 ~25 kDa	Outer membrane protein H [Pasteurella multocida] Acc. number EJZ80794	35652.51	9.75	51.65%	232
	39 kDa adhesive protein [Pasteurella multocida] Acc. number ABR27206	35248.11	9.14	36.42%	208
	Hypothetical protein AAUPMB_21592, partial [Pasteurella multocida subsp. multocida str. Anand1_buffalo]	15131	N.A.	N.A.	66
	Hypothetical protein AAUPMB_08244, partial [Pasteurella multocida subsp. multocida str. Anand1_buffalo]	20826	N.A.	N.A.	103

IR1 ~125–127 kDa	HgbA [Pasteurella multocida] Acc. number AAQ14873	110664.92	9.04	34.91%	229
	TonB-dependent hemoglobin/transferrin/lactoferrin receptor family protein [Pasteurella multocida subsp. multocida str. 3480] Acc. number YP006239903	11068.5	9.15	33.23%	179
	Outer membrane receptor protein, mostly iron transport [Pasteurella multocida subsp. gallicida P1059] Acc. number ZP_18468195	110193.67	9.16	34.92%	229
	iroA [Pasteurella multocida] Acc. number CAD58140	110287.54	8.74	28.96%	194
	Hypothetical protein AAUPMB_19760, partial [Pasteurella multocida subsp. multocida str. Anand1_buffalo]	50170			97

IR2 95–97 kDa	Ligand-gated channel protein [Pasteurella multocida] Acc. number WP_005752163	95786	9.07	37%	127
	Heme acquisition system receptor [Pasteurella multocida subsp. multocida str. HN06] Acc. number YP_005364287	95788	9.14	35%	126
	TonB-dependent lactoferrin and transferrin receptor [Pasteurella multocida subsp. multocida str. P52VAC] Acc. number ZP_15715832	87808.22	9.64	48.82%	263
	Transferrin binding protein A [Pasteurella multocida] Acc. number CAD90055	89284.06	9.71	48.39%	266

IR3 ~75 kDa	TonB-dependent lactoferrin and transferrin receptor [Pasteurella multocida subsp. multocida str. P52VAC] Acc. number ZP_15715832	87808.22	9.64	54.97%	372
	Transferrin binding protein A [Pasteurella multocida] Acc number CAD90055	89284.06	9.71	52.25%	357
	hmbR [Pasteurella multocida] Acc. number CAD58169	77413.93	9.75	44.25%	318

IR4 ~50 kDa	Long-chain fatty acid transport protein [Pasteurella multocida subsp. gallicida P1059] Acc. No. EJZ79884	47545.25	9.44	40.63%	144
	47 kDa outer membrane protein [Pasteurella multocida subsp. multocida str. 3480] Acc. number YP_006239073	47270.14	9.34	41%	144
	Hypothetical protein PM1069 [Pasteurella multocida subsp. multocida str. Pm70] Acc. number NP_246006	47831.56	9.57	41.31%	144
	Membrane protein, aromatic hydrocarbon degradation family [Pasteurella multocida 36950], Acc. number YP_005176018	46776	9.04	47%	107
	Translocation protein TolB [Pasteurella multocida] Acc. number WP_016533064	46017	9.04	33%	67
	HexD, partial [Pasteurella multocida], Acc. number WP_005752881	40030	9.34	46%	89
	TolC protein [Pasteurella multocida subsp. multocida str. 3480] Acc. number YP_006239672	43588	9.44	34%	64
	CexD [Pasteurella multocida] Acc. number AAF67275	42856	9.28	43%	86
	Outer membrane protein A [Pasteurella multocida] Acc. number AEC04319	38459.85	9.54	82.40%	676
	Hypothetical protein PM0786 [Pasteurella multocida subsp. multocida str. Pm70] Acc. number NP_245723	38121.61	9.44	66.86%	459

IR5 ~40 kDa	Outer membrane protein H [Pasteurella multocida] Acc. number EJZ80794	33740.43	9.58	44.09%	376
	Adhesive protein [Pasteurella multocida] Acc. number ABX58059	33740.43	9.58	66.13%	449
	Hypothetical protein AAUPMB_21592, partial [Pasteurella multocida subsp. multocida str. Anand1_buffalo]	15131	N.A.	N.A.	66
	Hypothetical protein AAUPMB_08244, partial [Pasteurella multocida subsp. multocida str. Anand1_buffalo]	20826	N.A.	N.A.	103

IR6 ~33 kDa	39 kDa adhesive protein [Pasteurella multocida] Acc. number ABR27206	35248.11	9.14	57.41%	421
	Outer membrane protein A [Pasteurella multocida] Acc. number ADF95751	38433.84	9.54	51.12%	178
	Outer membrane protein A [Pasteurella multocida] Acc. number ADF95751	38433.84	9.54	51.12%	178

IR7 ~30 kDa	Outer membrane protein H [Pasteurella multocida] Acc. no. CBN80564	33740.43	9.58	28.12%	138

IR8 ~25 kDa	MltA-interacting MipA protein, partial [Pasteurella multocida], Acc. number WP_020751274	24721	9.62	55%	66
IR8 ~25 kDa	Hypothetical protein AAUPMB_08244, partial [Pasteurella multocida subsp. multocida str. Anand1_buffalo]	20826	9.62	55%	95

Bands correspond to Figure 1.