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The Scientific World Journal
Volume 2014 (2014), Article ID 797824, 8 pages
Research Article

Protective Effect of Hesperetin and Naringenin against Apoptosis in Ischemia/Reperfusion-Induced Retinal Injury in Rats

1Department of Ophthalmology, Canakkale Onsekiz Mart University, Faculty of Medicine, 17060 Canakkale, Turkey
2Department of Histology and Embryology, Trakya University, Faculty of Medicine, 22030 Edirne, Turkey
3Deparment of Anesthesiology, Dokuz Eylül University, Faculty of Medicine, 35100 Izmir, Turkey

Received 2 December 2013; Accepted 22 December 2013; Published 30 January 2014

Academic Editors: S. Sivaprasad and Y. Zhong

Copyright © 2014 Selcuk Kara et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Purpose. Hesperetin and naringenin are naturally common flavonoids reported to have antioxidative effects. This study was performed to investigate whether either hesperetin or naringenin has a protective effect against apoptosis on retinal ischemia/reperfusion (I/R) injury. Methods. Retinal I/R was induced by increasing the intraocular pressure to 150 mmHg for 60 minutes. Thirty-three male Wistar albino rats were randomised into 5 groups named control, I/R + sham, I/R + solvent (DMSO), I/R + hesperetin, and I/R + naringenin. Animals were given either hesperetin, naringenin, or the solvent intraperitoneally immediately following reperfusion. Thickness of retinal layers and retinal cell apoptosis were detected by histological analysis, tunel assay, and immunohistochemistry assay. Results. Hesperetin and naringenin attenuated the I/R-induced apoptosis of retinal cells in the inner and outer nuclear cells of the rat retina. Retinal layer thickness of the naringenin treatment group was significantly thicker than that of the hesperetin, sham, and solvent groups ( ). Conclusions. Hesperetin and naringenin can prevent harmful effects induced by I/R injury in the rat retina by inhibiting apoptosis of retinal cells, which suggests that those flavanones have a therapeutic potential for the protection of ocular ischemic diseases.