Rapid Detection and Differentiation of Clonorchis sinensis and Opisthorchis viverrini Using Real-Time PCR and High Resolution Melting Analysis

Figure 2

Real-time PCR and HRM analysis of serial diluted genomic DNA. (a) A linear regression of the data providing a formula of , template dilutions 10 ng/μL to 1 pg/μL of C. sinensis. (b) A linear regression of the data providing a formula of , template dilutions 10 ng/μL to 1 pg/μL of O. viverrini. (c) Amplification curves of diluted genomic DNA of C. sinensis; 1–6: dilutions 10 ng/μL to 1 pg/μL. (d) Amplification curves of diluted genomic DNA of O. viverrini; 1–6: dilutions 10 ng/μL to 1 pg/μL. (e) Melting peaks of amplicon of diluted genomic DNA from two liver flukes (10 ng/μL to 1 pg/μL); 1: C. sinensis, 78.97 ± 0.11°C; 2: O. viverrini, 80.92 ± 0.08°C. (f) Normalized melting curves of diluted genomic DNA from two liver flukes (10 ng/μL to 1 pg/μL).

(a)

(b)

(c)

(d)

(e)

(f)