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Veterinary Medicine International
Volume 2010 (2010), Article ID 852146, 8 pages
Research Article

Immunization against Lamb Haemonchosis with a Recombinant Somatic Antigen of Haemonchus contortus (rHcp26/23)

1Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain
2Departamento de Enfermedades Transmisibles, Facultad de Veterinaria, Universidad del Zulia, 4005-A Maracaibo, Venezuela
3Departamento de ParasitologĂ­a, Facultad de Farmacia, Universidad de La Laguna, 38271 Tenerife, Spain

Received 28 January 2010; Accepted 30 April 2010

Academic Editor: Cynthia L. Baldwin

Copyright © 2010 Leticia García-Coiradas et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Haemonchosis, caused by the abomasal nematode Haemonchus contortus, is a common parasitic disease of sheep. Our previous results showed that a soluble fraction from adult stages of the nematode (p26/23) induced partial protection against challenge. Recombinant DNA technology was applied to obtain a synthetic protein (rHcp26/23). Immunological assays (ELISA, Western blotting, and immunolocalization), using sera from lambs immunized with p26/23, confirmed the identity of the recombinant protein and demonstrated that the synthetic protein is equivalent to the purified protein employed in the previous immunoprophylaxis studies. Vaccination of lambs with 300  𝜇 g of rHcp26/23 and Freund's adjuvant elicited a notable specific antibody response. Immunization did not induce any significant protection after challenge with 16000 infective larvae of H. contortus, and comparable values for parasite faecal egg output, packed cell volume, and abomasal parasite burdens were found in vaccinated and control animals.