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Veterinary Medicine International
Volume 2010, Article ID 852146, 8 pages
http://dx.doi.org/10.4061/2010/852146
Research Article

Immunization against Lamb Haemonchosis with a Recombinant Somatic Antigen of Haemonchus contortus (rHcp26/23)

1Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain
2Departamento de Enfermedades Transmisibles, Facultad de Veterinaria, Universidad del Zulia, 4005-A Maracaibo, Venezuela
3Departamento de Parasitología, Facultad de Farmacia, Universidad de La Laguna, 38271 Tenerife, Spain

Received 28 January 2010; Accepted 30 April 2010

Academic Editor: Cynthia L. Baldwin

Copyright © 2010 Leticia García-Coiradas et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. P. H. Holmes, “Pathophysiology of nematode infections,” International Journal for Parasitology, vol. 17, no. 2, pp. 443–451, 1987. View at Google Scholar · View at Scopus
  2. F. Jackson and R. L. Coop, “The development of anthelmintic resistance in sheep nematodes,” Parasitology, vol. 120, pp. S95–S107, 2000. View at Google Scholar · View at Scopus
  3. N. Bakker, L. Vervelde, K. Kanobana et al., “Vaccination against the nematode Haemonchus contortus with a thiol-binding fraction from the excretory/secretory products (ES),” Vaccine, vol. 22, no. 5-6, pp. 618–628, 2004. View at Publisher · View at Google Scholar · View at Scopus
  4. I. A. Domínguez-Toraño, M. Cuquerella, MA. T. Gómez-Muñoz, S. Méndez, F. J. Fernández-Pérez, and J. M. Alunda, “Vaccination of Manchego lambs against Haemonchus contortus with a somatic fraction (p26/23) of adult parasites,” Parasite Immunology, vol. 22, no. 3, pp. 131–138, 2000. View at Publisher · View at Google Scholar · View at Scopus
  5. E. A. Munn, T. S. Smith, M. Graham, C. A. Greenwood, A. S. Tavernor, and G. Coetzee, “Vaccination of merino lambs against haemonchosis with membrane-associated proteins from the adult parasite,” Parasitology, vol. 106, no. 1, pp. 63–66, 1993. View at Google Scholar · View at Scopus
  6. E. A. Munn, T. S. Smith, M. Graham, A. S. Tavernor, and C. A. Greenwood, “The potential value of integral membrane proteins in the vaccination of lambs against Haemonchus contortus,” International Journal for Parasitology, vol. 23, no. 2, pp. 261–269, 1993. View at Publisher · View at Google Scholar · View at Scopus
  7. W. D. Smith and D. S. Zarlenga, “Developments and hurdles in generating vaccines for controlling helminth parasites of grazing ruminants,” Veterinary Parasitology, vol. 139, no. 4, pp. 347–359, 2006. View at Publisher · View at Google Scholar · View at Scopus
  8. H. D. F. H. Schallig and M. A. W. Van Leeuwen, “Protective immunity to the blood-feeding nematode Haemonchus contortus induced by vaccination with parasite low molecular weight antigens,” Parasitology, vol. 114, no. 3, pp. 293–299, 1997. View at Publisher · View at Google Scholar · View at Scopus
  9. D. P. Jasmer and T. C. McGuire, “Protective immunity to a blood-feeding nematode (Haemonchus contortus) induced by parasite gut antigens,” Infection and Immunity, vol. 59, no. 12, pp. 4412–4417, 1991. View at Google Scholar · View at Scopus
  10. D. P. Knox, D. L. Redmond, G. F. Newlands, P. J. Skuce, D. Pettit, and W. D. Smith, “The nature and prospects for gut membrane proteins as vaccine candidates for Haemonchus contortus and other ruminant trichostrongyloids,” International Journal for Parasitology, vol. 33, no. 11, pp. 1129–1137, 2003. View at Publisher · View at Google Scholar · View at Scopus
  11. P. Willadsen, G. A. Riding, R. V. McKenna et al., “Immunologic control of a parasitic arthropod. Identification of a protective antigen from Boophilus microplus,” Journal of Immunology, vol. 143, no. 4, pp. 1346–1351, 1989. View at Google Scholar · View at Scopus
  12. E. A. Munn, “Rational design of nematode vaccines: hidden antigens,” International Journal for Parasitology, vol. 27, no. 4, pp. 359–366, 1997. View at Publisher · View at Google Scholar · View at Scopus
  13. I. A. Domínguez-Toraño, F. J. Fernández-Pérez, M. T. Gómez-Muñoz, J. M. Alunda, and M. Cuquerella, “Humoral and cellular response in lambs vaccinated against Haemonchus contortus with p26/23,” Small Ruminant Research, vol. 50, no. 1-2, pp. 29–37, 2003. View at Publisher · View at Google Scholar · View at Scopus
  14. L. García-Coiradas, F. Angulo-Cubillán, S. Méndez et al., “Isolation and immunolocalization of a putative protective antigen (p26/23) from adult Haemonchus contortus,” Parasitology Research, vol. 104, no. 2, pp. 363–369, 2009. View at Publisher · View at Google Scholar · View at Scopus
  15. D. Carr, The Handbook of Analysis and Purification of Peptides and Proteins by Reverse-Phase HPLC, Grace Vydac, 3rd edition, 2002.
  16. B. Szewczyk and D. F. Summers, “Efficient elution of purified proteins from polyvinylidene difluoride membranes (Immobilon) after transfer from SDS-PAGE and their use as immunogens,” in Methods in Molecular Biology, M. Manson, Ed., pp. 7–12, The Humana Press, Totowa, NJ, USA, 1992. View at Google Scholar
  17. P. Chomczynski and N. Sacchi, “Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction,” Analytical Biochemistry, vol. 162, no. 1, pp. 156–159, 1987. View at Google Scholar · View at Scopus
  18. J. Sambrook, E. F. Fritsch, and T. Maniatis, Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, New York, NY, USA, 2nd edition, 1989.
  19. MAFF (Ministry of Agriculture, Fisheries and Food, “Manual of Veterinary Parasitological Laboratory Techniques,” HMSO, London, 1971.
  20. J. M. Alunda, F. Angulo-Cubillán, and M. Cuquerella, “Immunization against ovine haemonchosis with three low molecular weight somatic antigens of adult Haemonchus contortus,” Journal of Veterinary Medicine B, vol. 50, no. 2, pp. 70–74, 2003. View at Publisher · View at Google Scholar · View at Scopus
  21. W. J. Coadwell and P. F. Ward, “Observations on the development of Haemonchus contortus in young sheep given a single infection,” Parasitology, vol. 71, no. 3, pp. 505–515, 1975. View at Google Scholar · View at Scopus
  22. H. D. F. H. Schallig, M. A. W. Van Leeuwen, B. E. Verstrepen, and A. W. C. A. Cornelissen, “Molecular characterization and expression of two putative protective excretory secretory proteins of Haemonchus contortus,” Molecular and Biochemical Parasitology, vol. 88, no. 1-2, pp. 203–213, 1997. View at Publisher · View at Google Scholar · View at Scopus
  23. S. E. Newton and E. N. T. Meeusen, “Progress and new technologies for developing vaccines against gastrointestinal nematode parasites of sheep,” Parasite Immunology, vol. 25, no. 5, pp. 283–296, 2003. View at Publisher · View at Google Scholar · View at Scopus
  24. D. P. Knox, D. L. Redmond, P. J. Skuce, and G. F. J. Newlands, “The contribution of molecular biology to the development of vaccines against nematode and trematode parasites of domestic ruminants,” Veterinary Parasitology, vol. 101, no. 3-4, pp. 311–335, 2001. View at Publisher · View at Google Scholar · View at Scopus
  25. E. Claerebout, D. P. Knox, and J. Vercruysse, “Current research and future prospects in the development of vaccines against gastrointestinal nematodes in cattle,” Expert Review of Vaccines, vol. 2, no. 1, pp. 147–157, 2003. View at Publisher · View at Google Scholar · View at Scopus
  26. J. P. Dalton, P. J. Brindley, D. P. Knox et al., “Helminth vaccines: from mining genomic information for vaccine targets to systems used for protein expression,” International Journal for Parasitology, vol. 33, no. 5-6, pp. 621–640, 2003. View at Publisher · View at Google Scholar · View at Scopus
  27. M. T. Gomez-Muñoz, M. Cuquerella, and J. M. Alunda, “Identification and partial purification of a 26 kilodalton antigen of adult Haemonchus contortus,” International Journal for Parasitology, vol. 26, no. 3, pp. 311–318, 1996. View at Publisher · View at Google Scholar · View at Scopus
  28. S. Yanming, Y. Ruofeng, C. I. Muleke, Z. Guangwei, X. Lixin, and L. Xiangrui, “Vaccination of goats with recombinant galectin antigen induces partial protection against Haemonchus contortus infection,” Parasite Immunology, vol. 29, no. 6, pp. 319–326, 2007. View at Publisher · View at Google Scholar · View at Scopus
  29. P. Geldhof, V. De Maere, J. Vercruysse, and E. Claerebout, “Recombinant expression systems: the obstacle to helminth vaccines?” Trends in Parasitology, vol. 23, no. 11, pp. 527–532, 2007. View at Publisher · View at Google Scholar · View at Scopus