Evaluation of In-House ELISA for Antirabies Antibodies Detection in Domestic Canine

Table 9

Assay performance of in-house ELISA BukTi-Vet under different skill operators.

	Original (%)^§		Operator 1 (%)		Operator 2 (%)		Operator 3 (%)
	Mean	95% CI	Mean	95% CI	Mean	95% CI	Mean	95% CI
Sensitivity	98.19	93.64–99.78	98.25	90.61–99.96	96.49	87.89–99.57	98.25	90.61–99.96
Specificity	97.87	88.71–99.95	91.30	71.96–98.93	91.30	71.96–98.93	78.26	56.29–92.54
Accuracy	98.10	94.55–99.61	96.25	89.43–99.22	95.00	87.69–98.62	92.50	84.39–97.19
PPV	99.09	94.00–99.87	96.55	88.16–99.06	96.49	87.96–99.04	91.80	83.75–96.05
NPV	95.83	85.34–98.91	95.46	74.98–99.33	91.30	72.79–97.63	94.74	71.82–99.22
LR+	46.15	6.64–320.91	11.29	3.00–42.49	11.09	2.95–41.76	4.52	2.08–9.82
LR−	0.02	0.01–0.07	0.02	0.003–0.14	0.04	0.01–0.15	0.02	0.003–0.16
AUC	0.98^a	0.95–0.99	0.95^a,b	0.87–0.99	0.94^a,b	0.86–0.98	0.88^b	0.79–0.94
CUI +ve	0.97	0.95–0.99	0.95	0.89–0.99	0.93	0.87–0.99	0.90	0.83–0.97
CUI −ve	0.94	0.89–0.98	0.87	0.78–0.96	0.83	0.73–0.93	0.74	0.61–0.87

Note. ^§Original diagnostic performance as described in Table 1. All data in the table are expressed in percent except those marked with an asterisk (). PPV, positive predictive value; NPV, negative predictive value; LR, likelihood ratio; AUC, area under curve; CUI, clinical utility index. AUC with the same notations showed a not significant difference (). Benchmark of CUI according to Mitchell [19]: excellent utility (≥0.81), good utility (≥0.64), fair utility (≥0.49), poor utility (<0.49), and very poor utility (<0.36).