Research Article
Multienzyme Modification of Hemp Protein for Functional Peptides Synthesis
Table 2
Specificity of the enzyme system (papain, Protease N Amano G, and Protease A Amano 2G) used for hydrolysis of hemp protein.
| | Enzyme | Source | Nature | Optimum temperature/pH | Activity |
| Papain
(E.C. 3.4.22.2) | Papaya | Cysteine protease | 55–60°C/pH 5–7 | 10,000 U/g |
| Protease N Amano G
(E.C. 3.4.24.28) | Bacillus subtilis | Metalloendopeptidase | 55°C/pH 7.5 | >150,000 U/g |
| Protease A Amano 2G
(E.C. 3.4.24.39) | Aspergillus oryzae | Metalloendopeptidase | 50°C/pH 7.0 | 20,000 U/g |
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