Characterizing Wool Keratin

Table 1

Keratin-associated proteins identified by database search in NCBInr (http://www.ncbi.nlm.nih.gov/) after trypsin digestion.

Protein	Peptide sequence
Type II keratin intermediate filament protein	HGETLRR; LEEEEIR;
	FAAFIDKVR; RLYEEEIR;
	AKQDM(Ox)ACLLK;
	DVDC*AYLRK; RYEEEVALR;
	TKEEINELNR; SRAEAESWYR;
	LGLDIEIATYRR; VIQRLTAEVENAK;
	FLEQQNKLLETK;
	SDLEANVEALIQETDFLR;
	KSDLEANVEALTQEIDFLR;
	LYEEEIRVLQAHISDTSVIVK
Type II keratin micro- fibrillar, component 7c protein	HGETLRR;
	LLEGEEQR;
	FAAFIDKVR;
	KDVDC*AYVR;
	DVDC*AYVRK;
	AKQDM(Ox)ACLLK;
	TKEEINELNR;
	LGLDIEIATYRR;
	VLQANISDTSVIVKM(Ox)DNSR
	KSDLEANVEALTQEIDFLR;
	SDLEANSEALIQEIDFLRR;
	LEAAVTQAEQQGEVALNDARC*K
Type II keratin micro- fibrillar, low-sulfur protein	LVVQIDNAK;
	DVEEWYIR;
	QLVEADLNGLRR;
	AQYEALVETNRR;
	QNQEYQVLLDVR;
	TVNALEVELQAQHNLR;
	DLERQNQEYQVLLDVR;
	LPCNPC^∗ATTNTC^∗GKPIGPC^∗ ISNPC^∗VSRTR

M(Ox) corresponds to oxidized methionine and C* corresponds to cysteine carbamidomethyl derivative.