Reexamination In Vitro and In Situ of an Antibacterially Modified Experimental Dental Resin Composite with Molecular Methods: A Pilot Study

Table 4

Design of the specific primers and probes.

Species/acc. number	Gene	Primers/probes	Sequences (5′-3′)	Amplicon length/bp	References
Eubacteria	16S	Euba-F	TGGAGCATGTGGTTTAATTCGA	143	This study
X58308	rDNA	Euba-R	TGCGGGACTTAACCCAACA
		Euba-S	TexRed- CACGAGCTGACGACARCCATGCA- BHQ2
Actinomyces spp.	16S	Act-F	GGT CTC TGG GCC GTT ACT GA	112	Bizhang et al., 2011 [35]
AJ635359	rDNA	Act-R1	TGG CCC CCA CAC CTA GTG
		Act-R2	GRC CCC CCA CAC CTA GTG
		Act-S	CTG GTA GTC CAC GCC GTA AAC GTT GG
A. odontolyticus	16S	Act-F	GGT CTC TGG GCC GTT ACT GA	123	This study
AB818950	rDNA	Aodo-R	CGCAGAAACCACGGGTGG
		Act-S	CTG GTA GTC CAC GCC GTA AAC GTT GG
A. naeslundii	16S	Anae-F	GGGCCTGGGAAAGATTG	131	This study
M33911	rDNA	Anae-R	TGACCGTGCACCCTCTCA
	(3 pro genome)	Anae-S	FAM-TTTTTGGTGTTTTTGGTGGGGGATC-BHQ-1
A. viscosus	nanH	Avis H-F	GTCGACAACCAGACGGGC	76	This study
EU805603	(1 pro genome)	Avis H-R	CGGCCTGTGAGTGGCC
		Avis H-S	FAM-TTCCACGTCAAGTCCTTCGACCAGG-BHQ-1
S. gordonii	gtfG	Sgor-F	AGCTAGTGCGGCAACCAATC	87	This study
U12643	(1 pro genome)	Sgor-R	TGTCTGGAGTCAGTTCTTGAAGTTTC
		Sgor-S	FAM-AGCTAACGAACAGACGACGCAACAGGAT-BHQ1
S. mitis	16S	Smit-F	GAGCTTGCTTCTCCGGATGA	141	This study
AB682354	(4 pro genome)	Smt-R	AATTGCACCTTTTAAGCAAATGTCA
		Smit-S	Hex-TTGCGAACGGGTGAGTAACGCGT-BHQ1
S. oralis	gtfR	Sora-F	GGCCCTTATTGTAGCACCAAAG	101	This study
AB025228	(1 pro genome)	Sora-R	TCTGAGCTGTAGCAGGTTCTTGA
		Sora-S	FAM-TCCATGCGGATGATGTAAAGCAGGTTGTAG-BHQ1
S. sanguinis	gtfP	Ssan-F	GCAAAAAAGACTGTTACAGACAARATTG	96	This study
AB252650	(1 pro genome)	Ssan-R	AGCTATCGCTCCCTGTCTTTGA
		Ssan-S	HEX-AGGTTGCAAAGAAAGATCGCTTGCCA-BHQ1