Initial Cytotoxicity of Mineral Trioxide Aggregate (MTA) during Setting on Human Mesenchymal Stem Cells

Figure 4

Live and dead cells of hMSCs exposed to 12.5% and 25% concentrations of extracts from each MTA before and after setting. Live (green) and dead (red) cells were observed by the fluorescent microscope. Washing steps were performed before live and dead staining to make sure that only intact live cells remained on the plate. Because the dead cells (red colored) were easily detached during the washing step, they are hardly seen in the current images.