Chelating Effect of Cellulose Acetate Hydrogel Crosslinked with EDTA Dianhydride Used as a Platform for Cell Growth

Figure 8

Confocal microscopy analysis of ASC cells adhered to HAC-EDTA at culture times of 1, 7, and 14 days. (a) Cells with low concentration of Ca²⁺ and Mg²⁺ on the first day of culture, which represents difficulty in adhesion of cells to the surface of the material. (b) Adhesion was higher when Ca²⁺ and Mg²⁺ were added to the material, presenting a larger number of cells on 7th day of culture. (c) After 14 days, the cells increased in quantity and continued to have morphology of the cell type.

(a)

(b)

(c)