Advances in Materials Science and Engineering

Advances in Materials Science and Engineering / 2019 / Article
Special Issue

Synthesis and Application of Novel Hybrid Nanomaterials in Catalysis, Adsorption, and Electrochemistry

View this Special Issue

Research Article | Open Access

Volume 2019 |Article ID 8781013 | https://doi.org/10.1155/2019/8781013

Dang Xuan Du, Bui Xuan Vuong, "Study on Preparation of Water-Soluble Chitosan with Varying Molecular Weights and Its Antioxidant Activity", Advances in Materials Science and Engineering, vol. 2019, Article ID 8781013, 8 pages, 2019. https://doi.org/10.1155/2019/8781013

Study on Preparation of Water-Soluble Chitosan with Varying Molecular Weights and Its Antioxidant Activity

Academic Editor: Hien Duy Mai
Received02 Jan 2019
Accepted13 Feb 2019
Published20 Mar 2019

Abstract

The preparation of water-soluble chitosan (WSC) with various molecular weights by gamma Co-60 irradiation of chitosan solution (5%) in the presence of hydrogen peroxide (1%) combined with acetylated reaction was carried out. The average molecular weight (Mw) of chitosan was measured by gel permeation chromatography (GPC). The chemical structure and the crystallinity of chitosan and WSC were characterized by Fourier-transform infrared (FT-IR) spectroscopy and X-ray diffraction (XRD), respectively. The antioxidant activity of WSC and chitosan was investigated using the free radical 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS•+). Obtained results showed that chitosan with Mw of 18–90 kDa could be efficiently prepared by this technique in the dose range from 10 to 24.5 kGy. After the acetylated process, the resulting WSC possesses good solubility in a wide pH level of 2–12, and WSC with low molecular weight exhibited significantly higher antioxidant activity than the one with high molecular weight. In detail, the antioxidant activity was 14.7%, 70.5%, 84.2%, 89.4%, and 97.5% for WSC samples prepared from chitosan with Mw of 140.2 kDa, 91.4 kDa, 51.2 kDa, 35.3 kDa, and 18.1 kDa, respectively, at the same reaction time of 90 min. Moreover, the antioxidant activity of WSC was higher than that of chitosan. Thus, WSC prepared by gamma Co-60 irradiation and acetylated process can be potentially applied as a natural antioxidant agent.

1. Introduction

Antioxidant is construed as any substance that inhibits oxidation or reactions promoted by oxygen, peroxides, or free radicals. Nowadays, synthetic antioxidants such as t-butylhydroquinone, propyl gallate, butylated hydroxytoluene and butylated hydroxyanisole have been diversely utilized to counteract the deterioration of stored food and preserve cosmetics [1]. However, the application of synthetic antioxidants requites a strict adherence in utilized dose due to its possibly harmful side effects to the human health. Hence, study on extraction and application of antioxidants from natural origin to alternative to synthetic antioxidants was very necessary [24].

Chitosan is a natural polymer which has structure of polysaccharide consisting of N-glucosamine and N-acetylglucosamine units linked by β-1,4 glycoside. This polymer has been widely applied in many fields owing to its unique properties such as biocompatibility, biodegradability, nontoxicity, antibacterial, antifungal, and antitumor. Moreover, chitosan also has the ability to stimulate disease resistance and heal the wound quickly [5]. However, a significant drawback of chitosan is it is soluble only in diluted-acid media. This cause restricted applications of chitosan, and therefore, modification of chitosan with the aim of enhancing its solubility has attracted a great deal of research attention [3, 6]. Recently, studies on chitosan modified with resultant products as natural antioxidants were carried out [2, 79]. However, these studies have only focused on preparation of low molecular weight chitosan or oligomers due to their easy solubility in water and high antioxidant activity [10]. In addition, the antioxidant activity of high molecular weight chitosan [8] and chitosan derivatives [11] has also been investigated. Generally, the lower the molecular weight of the chitosan, the higher the solubility obtained [9]. This means that, chitosan with low molecular weight had a merit in applied ability due to soluble potency. However, if molecular weight was too low, like chitosan oligomer, it was also able to be a demerit in case of utilizing as a stabilizer to prepare metal nanoparticles; then, a high molecular weight of polymers had a better stability. Consequently, the preparation of water-soluble chitosan with molecular weight that was higher than chitosan oligomers for adding information and extending application was a necessary study.

The chitosan with varying molecular weight could be prepared from initial chitosan by a variety of techniques. Among them, gamma irradiation and hydrogen peroxide have some merits such as environment-friendly and being suitable for production of high-purity products and industrial manufacturing [12]. Furthermore, the simultaneous combination of gamma irradiation and hydrogen peroxide could efficiently degrade chitosan and reduce required dose owing to synergistic effect [13].

In this study, chitosan with varying molecular weight was prepared by hydrogen peroxide and gamma irradiation of chitosan solution containing hydrogen peroxide. Then, degraded chitosan has been acetylated by acetic anhydride to prepare water-soluble chitosan. The structure and solubility of resultant water-soluble chitosan have been investigated. Moreover, the antioxidant activity of water-soluble chitosan with varying molecular weight has also been evaluated.

2. Materials and Methods

2.1. Materials

Chitosan derived from shrimp shell with a weight average molecular weight (Mw) of 140.1 kDa and degree of deacetylation (DD%) of 91.3% was supplied by a company in Vung Tau province, Vietnam. Hydrogen peroxide (30%) was purchased from Merck, Germany. 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) was supplied by Biobasic, Canada. The other chemicals such as NaOH, NH4OH (25%), lactic acid, and ethyl alcohol were of reagent grade.

2.2. Preparation of Chitosan with Varying Molecular Weights

In this study, the degradation of chitosan was carried out in two steps. In the first step, initial chitosan (CTS0) was degraded in the swollen state by using 1% hydrogen peroxide with the ratio of chitosan/H2O2 of 1/10 (w/v) for 24 h. The obtained product (CTS1) was washed by distilled water and then dried naturally. In the second step, CTS1 was dissolved in 3% lactic acid and stirred for 2 h. An amount of hydrogen peroxide was added in the mixture to create solution with concentration of chitosan, lactic acid, and H2O2 to be 5%, 2.5%, and 1% (w/v), respectively. Finally, this resulting mixture was irradiated by gamma SVST Co-60/B source with a dose rate of 1.33 kGy/h in the range of dose up to 24.5 kGy. The obtained chitosan at the different doses of this irradiated process, CTS2 (3.5 kGy), CTS3 (10.5 kGy), and CTS4 (24.5 kGy), has been filtered by a stainless steel mesh and neutralized by 5% NH4OH (v/v). Ethyl alcohol was added in solution during stirring to precipitate chitosan. This precipitant was filtered and washed several times with alcohol and then dried in a force air oven at 60°C. The samples of dried chitosan were ground into powder for GPC measurement and preparation of water-soluble chitosan.

2.3. Preparation of Water-Soluble Chitosan

The chitosan samples from CTS0 to CTS4 were dissolved in lactic acid to create 150 mL solution with chitosan concentration of 5%. 40.5 mL ethyl alcohol and 4.5 mL anhydride acetic were added in solution. Then, the solution was stirred for 2 h and kept at ambient temperature overnight for acetylated process. The solutions after reaction were filtered, precipitated, washed, and dried. The dried chitosan samples were also ground into powder for FT-IR, XRD measurements, and antioxidant activity investigations.

2.4. Characterization

The Mw of chitosan samples was determined by using a GPC (LC-20AB Shimadzu, Japan) using an ultra-hydrogel column model 250 of Waters (USA) and detector RI-10A. The calibration of column was carried out by using pullulan with Mw of 780–3,80,000 Da. The mobile phase was buffer solution 0.25 M CH3COOH/0.25 M CH3COONa with a flow rate of 1 mL/min [14] and temperature at 30°C. The concentration of chitosan sample was ca. 0.1% (w/v).

IR spectra were taken on an FT-IR 8400S spectrometer (Shimadzu, Japan) using KBr pellets. The DD% was calculated based on FT-IR spectra according to the following equation [15]:where and are the absorbances of chitosan at 1320 and 1420 cm−1, respectively.

X-ray diffraction (XRD) measurements of WSC were carried out on an X’Pert Pro X-ray diffractometer (PANalytical, Netherlands) and used a CuKα target at 45 kV-40 mA with a scattering range (2θ) of 5°–40°.

The water solubility of WSC was estimated based on the solution transmittance at 600 nm that was recorded on a UV-vis spectrophotometer (V630, Jassco, Japan) against 0.5% WSC solution (w/v) using a quartz cell with an optimal path length of 1 cm [16].

2.5. Antioxidant Assay

Antioxidant assay was carried out by dissolving 2,2-azino-bis (3-ethylbenzothiazoline-6-sunphonic acid) (ATBS) in water to create the solution with a concentration of 7.4 mM. Next, 2 mL ATBS solution was mixed with 2 mL K2S2O8 with a concentration of 2.6 mM to obtain the free radical cation ATBS•+. This ATBS•+ solution was kept in the dark for 24 h. Then, the ATBS•+ solution was diluted by water to receive an optical density (OD) of 1 ± 0.1 at a wavelength of 734 nm, after which the solution was also diluted with water for the control. For investigating antioxidant activity, the WSC samples with concentration of 0.2% were mixed with 0.1% acetic acid [9]. About 0.6 mL of solution sample was added into the cuvette that contained 1 mL of ABTS•+ solution. Added otherwise by 0.6 mL distilled water into a cuvette that contained 1 mL ABTS•+ solution, we could receive a control sample. The OD of the samples was measured over time by using a UV-vis spectrophotometer at a max absorbance wavelength of 734 nm [17]. The efficiency of free radical scavenging was calculated by the following equation:where is the OD of the control sample (ABTS•+ and water without WSC) and ODAS is the OD of the solution containing ABTS•+ and WSC [17, 18].

2.6. Determination of Reducing Power

In order to clarify the rule of antioxidant activity in another way, the reducing power of WSC was also determined based on the ferricyanide method [19]. The WSC sample was diluted to create solution with 0.3% concentration (w/v). 1 mL of this solution was added to 2.5 mL of phosphate buffer (200 mM, pH = 6.6) and then 2.5 mL of potassium ferricyanide. This mixture was incubated for 20 min at 50°C in a water bath. Next, 2.5 mL of 10% trichloroacetic acid was added and then centrifuged at 3000 rpm for 10 min. 5 mL of upper layer solution was mixed with 1.0 mL of 0.1% ferric chloride and water. The obtained solution was measured for absorbance at 700 nm. The higher the absorbance, the stronger the reducing power obtained.

3. Results and Discussion

3.1. Preparation of Chitosan of Different Molecular Weights

According to the previous studies, the degradation of chitosan in the heterogeneous state in the presence of hydrogen peroxide solution is quite efficient [9, 20]. However, if the concentration of hydrogen peroxide was at the high level, the main chain structure of chitosan may be changed [20]. The way around this situation is the choice of 1% H2O2 [9, 13]. In addition, the degradation of chitosan in the heterogeneous state was almost supposed that it occurred in the amorphous region, and as a result, the reduction of molar mass of chitosan is only reached to a certain extent. Reasoning further along this line, the degradation of chitosan at crystal regions regularly occurs in the homogeneous state, i.e., in solution. For this study, the degradation of chitosan in the heterogeneous state and homogeneous state was performed in two steps in turn.

As shown in Table 1, the degradation of chitosan by 1% hydrogen peroxide is fairly efficient. Moreover, the DD of chitosan was almost unchanged. According to Qin et al. [20], the structure of the degraded chitosan with a Mw less than 50 kDa that was obtained by the high concentration of hydrogen peroxide as well as the long degradation time had been remarkably changed. Hence, the H2O2 concentration of 1% was chosen to prevent the change of the structure of chitosan. Moreover, the obtained chitosan with a molecular weight of 91 kDa was suitable for preparation of WSC with varying molecular weight. Since Mw was lower than 10 kDa, chitosan was in the form of an oligomer that may be soluble in water [9]; then, the acetylation of chitosan for its improvement of solubility was unnecessary.


First step (1% H2O2)Second step (1% H2O2 and γ-ray)
3.5 kGy10.5 kGy24.5 kGy
CTS0CTS1CTS2CTS3CTS4

Mw (kDa)140.191.751.735.118.6
DD (%)91.191.390.391.089.5

The further degradation of chitosan was carried out in solution by combination of H2O2 and gamma irradiation. The results showed that Mw of obtained chitosan at the different doses of 3.5 kGy, 10.5 kGy, and 24.5 kGy were 51.7 kDa, 35.1 kDa, and 18.6 kDa, respectively. Meanwhile, the DD of resulting chitosan samples was hardly different. This outcome indicated that at only 1% concentration of H2O2 combined with gamma ray, the reduction of molar mass is very efficient even when the applied dose is quite low, less than 25 kGy. Nguyen et al. [9] and Nguyen et al. [13] degraded chitosan by simultaneously combining H2O2 and gamma ray; as a result, they obtained chitosan oligomers at the low dose, about 12 kGy with 1% concentration of H2O2 used. Although these reductions were further than those in our study, the degree of Mw degradation (%) is almost the same owing to the fact that initial chitosan in those studies has different molecular weights as well as origin (i.e., crab shell in their studies and shrimp shell in this study). However, the degree of Mw reduction (%) in their studies and in this study is almost the same owing to the fact that initial chitosan in those studies was different in molecular weight as well as origin (from crab shell in their studies and shrimp shell in this study). The phenomenon of the increase in degraded efficiency with the presence of H2O2 was suggested through synergistic effect [13]. Accordingly, the hydroxyl radicals formed through the radiolysis of water and H2O2 are mainly responsible for degradation of chitosan:

Additionally formation of hydroxyl radicals, and can react with H2O2 during irradiation:

Hydroxyl radicals plays a significant role as a powerful oxidative agent, reacting with chitosan chain by abstraction of hydrogen atoms linked with carbon to form carbohydrate radicals (Figure 1) that can then cause the direct breakage of the glycosidic linkage by rearrangement [21]. The obtained chitosan samples with varying molecular weight and DD of ∼90% then were utilized for preparation of WSC through N-acetylation process.

3.2. Preparation of Water-Soluble Chitosan

The chitosan samples with different molar masses CTS0, CTS1, CTS2, CTS3, and CTS4 were N-acetylated by acetic anhydride to create WSC samples denoted by WSC0, WSC1, WSC2, WSC3, and WSC4, respectively. Unlike chitosan insoluble in water before reaction, all WSC samples shown in Figure 2 had good solubility in water despite different soluble time periods (Table 2). The transmittance (T) of all WSC samples was more than 98%. This means that water solubility of CTS was remarkably improved by virtue of N-acetylated process. Furthermore, these WSC samples also were soluble in the slightly alkaline media (pH of 7–12) regardless of its different molecular weights (data not showed). According to the results previously published by Lu et al. [6], the impact of molecular weight of WSC on solubility in water and in alkali media was ineffective and effective, respectively. In terms of the solubility in water, we have the similar result. For the alkaline media, the higher molecular weight of WSC has the lower solubility obtained [6]. However, all WSC samples in our study were soluble in weakly alkaline media. It may be due to the fact that the range of molecular weight in our study is far lower compared to that of their study. The WSC samples with Mw > 180 kDa in their study were slightly soluble in pH > 9 media. In our study, all WSC samples were prepared from chitosan with Mw less than 140 kDa, and therefore, they accounted for good solubility in dilute alkaline media. Feng et al. [3] also studied solubility of N-acetylated chitosan with DD∼50%, prepared by acetylation of chitosan degraded by cellulase enzyme. They reported that the only N-acetylated chitosan with low Mw (<18 kDa) has a high solubility and retained over a wide pH range. The solubility of the others (Mw > 18 kDa) in the alkaline decreased with increasing Mw. Unlike the results of Feng et al., WSC prepared in our study has a high solubility in over a wide pH range despite the MW from 18 to 140 kDa. Apart from the molecular weight, the solubility of chitosan depends on the other factors such as intermolecular interactions (van der Walls forces), intermolecular interaction forces, the degree of acetylation, and initial soluble media [2, 3].


SamplesDD (%)Soluble time at 25°C (h)T (%)

WSC056.32.599.93
WSC157.72.099.95
WSC252.32.099.97
WSC349.21.599.98
WSC448.71.599.98

The FT-IR spectra of the WSC samples as well as CTS0 (initial chitosan) and CTS1 (degraded by only H2O2) are shown in Figure 3. The broad band around 3427 cm−1 is assigned to O–H and N–H bond stretching. The band at 1159–895 cm−1 is assigned to the special absorption peaks of β (1–4) glycoside bond, which is characteristic of polysaccharide structure of chitosan [22]. The peaks at 1072 and 1028 cm−1 are assigned to stretching vibration of C–O and stretching of the C–O–C in the glucopyranose ring, respectively. Peaks at 1320 and 1420 cm−1 are assigned to the absorbance of C–N of CH3CONH (amid III) and symmetrical deformation of CH2 as well as CH3. The peaks at 1650 cm−1 and 1597 cm−1 are, respectively, concerned to stretching vibration of amide I and amide II groups [3]. The C–H stretching of methyl and methane were assigned at 2922 and 2876, respectively. In terms of mainly chemical structure, CTS1 is hardly different from CTS0. This accounts for unchanged DD (Table 1). Compared to initial chitosan (CTS0) and CTS1, the principle functional groups of WSC were still present after N-acetylation. This implied that the main polysaccharide chain structure remained after degradation and N-acetylation. However, there is change of absorbance at some bands. Especially, the peaks at 1650 and 1597 cm−1 owing to –CONH2 stretching vibration were significantly enhanced. This revealed that the acetylated reaction mainly occurred at the amino group of chitosan [15]. On the contrary, the absorbance at 2922 and 2876 cm−1 due to C–H stretching of methyl and methane were quite weakened. It indicated that N-acetylation reaction impacts on inter-macromolecular hydrogen bonds and interchain hydrogen bonds. This is due to of water solubility by virtue of reduced crystallinity of chitosan [6].

The X-ray diffraction (XRD) patterns of WSC and initial chitosan (CTS0) are shown in Figure 4. In the XRD pattern of CTS0, a broad peak of 2θ around 20° and another peak around 9° were assigned to the diffraction of the plane of the crystal region in the chitosan structure. This pattern refers to as the L-2 polymorph of chitosan [9, 23]. As can be seen in Figure 4, the diffraction intensity of peaks in WSC patterns is weaker than that of in CTS0—even disappearance at 2θ around 9°. These results showed that N-acetylation and degradation caused the destruction of the crystallinity of chitosan. Consequently, the lower degree of crystallinity of WSC obtained from these processes induced the higher solubility attained in aqueous media. This is similar to the previous reports [6, 13]. For the WSC1, the higher intensity can be observed at 2θ around 20° compared to WSC0 and CTS0. This result may be due to the fact that the first step of degradation by only H2O2 mainly occurs at the amorphous region. Accordingly, the obtained chitosan has relatively higher crystallinity similar to the outcome previously reported by Qin et al. [20].

3.3. Antioxidative Activity Assay

Free radicals containing reactive oxygen species are unstable agents. They react readily with other groups or substances in body, causing cell damage. Thus, one of the most effective defense mechanisms of the body against diseases is removal of free radicals by using antioxidants. In this study, the antioxidant activity of WSC was investigated by free-radical scavenging of ABTS•+. As can be seen in Figure 5, initial ABTS solution was transparent, whereas the color of ABTS•+ solution was dark green (Figure 5(a)). The color of ABTS•+ in aqueous solution was green. When ABTS•+ solution was added into WSC solution, a variation in color occurred. The color of ABTS•+ solution in the presence of WSC was decreased. Moreover, the ABTS•+ samples in WSC3 and WSC4 were almost colorless (Figure 5(b)). It proved that WSC scavenged ABTS•+. As a result, the green color of ABTS•+ solution in the presence of WSC was weaker than that of ABTS•+ without WSC. The higher the molecular weight of WSC was, the lower the free-radical scavenging activity attained. The efficiency of antioxidant activity is one of the parameters using to evaluate free-radical scavenging activity of WSC. In this study, the efficiency of antioxidant activity was calculated by equation (2) [9]. It was obvious that the efficiency of antioxidant activity of WSC samples increased in a time-dependent manner, and this parameter also increased versus the decrease of molecular weight (Figure 6). In particular, the WSC4 sample with the lowest molecular weight exhibited the highest efficiency of antioxidant activity, attained 92% after only 10 min and 98% after 90 min (Figure 6). Besides, the efficiency of antioxidant activity of the WSC0 sample was entirely lower than that of CTS4 sample and the others. The reason may be due to the significant difference of Mw. Feng et al. [3] also reported that the antioxidant activity of WSC with Mw of 281.0 and 1.8 kDa was significantly different. The WSC with Mw of 281 kDa has a relatively low antioxidant activity. Whereas, the other with Mw of 1.8 kD had a quite high antioxidant activity approximated with α-tocopherol [3]. The antioxidant activity of a compound was also suggested in various mechanisms such as prevention of chain initiation, decomposition of peroxides, biding of transition metal ion catalysts, radical scavenging, and reductive capacity [3].

Nguyen et al. [9] reported the preparation of water-soluble oligochitosan and its antioxidant activity. Results showed that water-soluble oligochitosan mainly prepared by degradation of chitosan using hydrogen peroxide and gamma irradiation at low dose <25 kGy. The obtained oligochitosan with Mw of 35.29 kDa, 7.03 kDa, and 4.07 kDa had the scavenge percentage against ATBS•+ radical in 90 min of 69.6%, 84.5%, and 99.2%, respectively. In terms of the rule of efficiency of antioxidant activity versus molecular weight of chitosan, their study was consistent with our study. However, the difference is that the Mw of oligochitosan in their study was lower compared to that in our study despite the same efficiency of antioxidant activity (more than 90%) and good solubility. Chitosan with low molecular weight such as oligochitosan (Mw less than 10 kDa) in studies reported by Je et al. [2], Feng et al. [3], and Nguyen et al. [9] generally had a good antioxidant activity. However, its antimicrobial property was less than that of chitosan with medium molecular weight (Mw∼10–100 kDa). In addition, in the case of chitosan using as a stabilizer for synthesis of metal nanoparticles, a high Mw has a better stability. In our study, WSC with a molecular weight of 18–90 kDa has good solubility in water so that it has suitably potential to be used as a stabilizer or an antioxidant substance. The other point is the difference of the method for preparation of water-soluble chitosan. In our method, apart from degradation by hydrogen peroxide and gamma Co-60 irradiation like the method in Nguyen et al. [9], chitosan also was acetylated to increase solubility and Mw was kept constant at medium molecular weight. Therefore, in terms of this approach, the obtained chitosan has good solubility and high antioxidant activity at the relatively higher molecular weight.

In terms of reason for antioxidant activity, many authors mentioned in their studies [3, 9, 24] that the hydroxyl and amino groups in chitosan molecules play important roles in free-radical scavenging activity. According to these authors, the more activated the hydroxyl and amino group are, the more the antioxidant activity. The hydroxyl and amino group could be activated in case intramolecular hydrogen bond and van der Waals forces reduced sharply. In our study, acetylated process destroyed crystallinity of chitosan as mentioned above. This may significantly decline intramolecular hydrogen bond. As a result, hydroxyl and amino groups could be more mobile. They facilitate effective reactions with ABTS•+ radical [11]. In addition, the residual hydroxyl groups in polysaccharides can react with radicals via a H abstraction. In a similar way, the residual free amino groups can absorb a hydrogen ion to form ammonium groups, which in turn react with radicals through an addition reaction [25].

The reducing capacity of a substance may be construed as a significant indicator of its potential antioxidant activity [26]. The substances with good reducing capacity are electron donors. They can easily react with free radicals to convert them to more stable states and terminate radical chain reactions [2]. As shown in Table 3, the reducing power of WSC samples increased with the decrease of Mw. It is obvious that reducing power of CST4 and WSC samples was in good agreement with the efficiency of antioxidant activity. The stronger the reducing power of WSC, the higher the efficiency of antioxidant activity attained. Besides, reducing power of CTS4 was also more than WSC0 but less than the others. It implicated that WSC has generally given a higher reducing power than chitosan. However, in this case, CTS4 having a higher reducing power than WSC0 may be due to the limit of Mw [8, 10, 27]. In WSC0 with the higher Mw, the structure chain was twisted making a mobile degree of amino and hydroxyl groups with decreasing vigour. It could be accounted for a negligible reducing power of WSC0. Rao et al. [26] studied irradiation of chitosan-glucose mixture and antioxidant activity of the resultant Maillard products. They reported that the mixture irradiated at high dose had a high reducing power. They also suggested that the intermediate reduction compounds of Maillard reaction products broke the radical chain by donation of a hydrogen atom [26].


SamplesWSC0WSC1WSC2WSC3WSC4CTS4

OD0.030.200.360.440.470.12

4. Conclusions

Water-soluble chitosan (WSC) with Mw in the range of 18–140 kDa was successfully prepared by combination of degradation and acetylation in which the gamma Co-60 irradiation and hydrogen peroxide were used to adjust the molecular weight. The solubility and antioxidant activity of the obtained WSC were significantly enhanced. All WSC samples showed good solubility in water with the transmittance more than 98%. The WSC4 sample exhibited the highest efficiency of antioxidant activity, attained 92% after only 10 min and 98% after 90 min. Owing to the water solubility and high efficiency of antioxidant activity, WSC can be potentially used for various purposes of applications, especially as an effective antioxidant or a stabilizer in synthesizing metal nanoparticles.

Data Availability

The data used to support the findings of this study are included within the article.

Conflicts of Interest

The authors declare that they have no conflicts of interest.

Acknowledgments

The authors would like to thank Professor Q. H. Nguyen (VINAGAMMA Center, Vietnam) for reading this manuscript.

References

  1. A. Wanita and K. Lorenz, “Antioxidant potential of 5-n-pentadecylresorsinol,” Journal of Food Processing and Preservation, vol. 20, no. 5, pp. 417–429, 1996. View at: Publisher Site | Google Scholar
  2. J.-Y. Je, P.-J. Park, and S.-K. Kim, “Radical scavenging activity of hetero-chitooligosaccharides,” European Food Research and Technology, vol. 219, no. 1, pp. 60–65, 2004. View at: Publisher Site | Google Scholar
  3. T. Feng, Y. Du, J. Li, Y. Wei, and P. Yao, “Antioxidant activity of half N-acetylated water-soluble chitosan in vitro,” European Food Research and Technology, vol. 225, no. 1, pp. 133–138, 2007. View at: Publisher Site | Google Scholar
  4. C. Schyvens, “Food additives: antioxidant,” Encyclopedia of Food Safety, vol. 2, pp. 455–458, 2014. View at: Google Scholar
  5. T. Dai, M. Tanaka, Y.-Y. Huang, and M. R. Hamblin, “Chitosan preparations for wounds and burns: antimicrobial and wound-healing effects,” Expert Review of Anti-infective Therapy, vol. 9, no. 7, pp. 857–879, 2011. View at: Publisher Site | Google Scholar
  6. S. Lu, X. Song, D. Cao, Y. Chen, and K. Yao, “Preparation of water-soluble chitosan,” Journal of Applied Polymer Science, vol. 91, no. 6, pp. 3497–3503, 2004. View at: Publisher Site | Google Scholar
  7. P.-J. Chien, F. Sheu, W.-T. Huang, and M.-S. Su, “Effect of molecular weight of chitosans on their antioxidative activities in apple juice,” Food Chemistry, vol. 102, no. 4, pp. 1192–1198, 2007. View at: Publisher Site | Google Scholar
  8. A. Wan, Q. Xu, Y. Sun, and H. Li, “Antioxidant activity of high molecular weight chitosan and N,O-quaternized chitosans,” Journal of Agricultural and Food Chemistry, vol. 61, no. 28, pp. 6921–6928, 2013. View at: Publisher Site | Google Scholar
  9. N. T. Nguyen, D. Q. Hoang, N. D. Nguyen, Q. H. Nguyen, and D. H. Nguyen, “Preparation, characterization, and antioxidant activity of water-soluble oligochitosan,” Green Processing and Synthesis, vol. 6, no. 5, pp. 461–468, 2017. View at: Publisher Site | Google Scholar
  10. T. Laokuldilok, T. Potivas, N. Kanha et al., “Physicochemical, antioxidant, and antimicrobial properties of chitooligosaccharides produced using three different enzyme treatments,” Food Bioscience, vol. 18, pp. 28–33, 2017. View at: Publisher Site | Google Scholar
  11. H.-Y. Lin and C.-C. Chou, “Antioxidative activities of water-soluble disaccharide chitosan derivatives,” Food Research International, vol. 37, no. 9, pp. 883–889, 2004. View at: Publisher Site | Google Scholar
  12. N. D. Nguyen, V. P. Dang, T. A. Nguyen, and Q. H. Nguyen, “Synergistic degradation to prepare oligochitosan by γ-irradiation of chitosan solution in the presence of hydrogen peroxide,” Radiation Physics and Chemistry, vol. 80, no. 7, pp. 848–853, 2011. View at: Publisher Site | Google Scholar
  13. Q. H. Nguyen, V. P. Dang, N. D. Nguyen, and T. K. L. Nguyen, “Degradation of chitosan in solution by gamma irradiation in the presence of hydrogen peroxide,” Carbohydrate Polymers, vol. 87, no. 1, pp. 935–938, 2012. View at: Publisher Site | Google Scholar
  14. J. Z. Knaul, M. R. Kasaai, V. T. Bui, and K. A. Creber, “Characterization of deacetylated chitosan and chitosan molecular weight review,” Canadian Journal of Chemistry, vol. 76, no. 11, pp. 1699–1706, 1998. View at: Publisher Site | Google Scholar
  15. J. Brugnerotto, J. Lizardi, F. M. Goycoolea, W. Argüelles-Monal, J. Desbrières, and M. Rinaudo, “An infrared investigation in relation with chitin and chitosan characterization,” Polymer, vol. 42, no. 8, pp. 3569–3580, 2001. View at: Publisher Site | Google Scholar
  16. M. Fan, Q. Hu, and K. Shen, “Preparation and structure of chitosan soluble in wide pH range,” Carbohydrate Polymers, vol. 78, no. 1, pp. 66–71, 2009. View at: Publisher Site | Google Scholar
  17. R. Re, N. Pellegrini, A. Proteggente, A. Pannala, M. Yang, and C. Rice-Evans, “Antioxidant activity applying an improved ABTS radical cation decolorization assay,” Free Radical Biology and Medicine, vol. 26, no. 9-10, pp. 1231–1237, 1999. View at: Publisher Site | Google Scholar
  18. K. Thaipong, U. Boonprakob, K. Crosby, L. Cisneros-Zevallos, and D. Hawkins Byrne, “Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts,” Journal of Food Composition and Analysis, vol. 19, no. 6-7, pp. 669–675, 2006. View at: Publisher Site | Google Scholar
  19. G.-C. Yen and P.-D. Duh, “Antioxidative properties of methanolic extracts from peanut hulls,” Journal of the American Oil Chemists’ Society, vol. 70, no. 4, pp. 383–386, 1993. View at: Publisher Site | Google Scholar
  20. C. Q. Qin, Y. M. Du, and L. Xiao, “Effect of hydrogen peroxide treatment on the molecular weight and structure of chitosan,” Polymer Degradation and Stability, vol. 76, no. 2, pp. 211–218, 2002. View at: Publisher Site | Google Scholar
  21. P. Ulanski and C. von Sonntag, “OH-Radical-induced chain scission of chitosan in the absence and presence of dioxygen,” Journal of the Chemical Society, Perkin Transactions, vol. 2, no. 10, pp. 2022–2028, 2000. View at: Publisher Site | Google Scholar
  22. L. Wei, Q. Li, W. Tan, F. Dong, F. Luan, and Z. Guo, “Synthesis, characterization, and the antioxidant activity of double quaternized chitosan derivatives,” Molecules, vol. 22, no. 3, p. 501, 2017. View at: Publisher Site | Google Scholar
  23. C. H. Hsu, S. K. Chen, W. Y. Chen, M. L. Tsai, and R. H. Chen, “Effect of the characters of chitosans used regeneration conditions on the yield and physicochemical characteristics of regenerated products,” International Journal of Molecular Sciences, vol. 16, no. 12, pp. 8621–8634, 2015. View at: Publisher Site | Google Scholar
  24. Y. Yang, R. Shu, J. Shao, G. Xu, and X. Gu, “Radical scavenging activity of chitooligosaccharide with different molecular weights,” European Food Research and Technology, vol. 222, no. 1-2, pp. 36–40, 2006. View at: Publisher Site | Google Scholar
  25. W. Xie, P. Xu, and Q. Liu, “Antioxidant activity of water-soluble chitosan derivatives,” Bioorganic & Medicinal Chemistry Letters, vol. 11, no. 13, pp. 1699–1701, 2001. View at: Publisher Site | Google Scholar
  26. M. S. Rao, S. P. Chawla, R. Chander, and A. Sharma, “Antioxidant potential of Maillard reaction products formed by irradiation of chitosan-glucose solution,” Carbohydrate Polymers, vol. 83, no. 2, pp. 714–719, 2011. View at: Publisher Site | Google Scholar
  27. K. W. Kim and R. L. Thomas, “Antioxidative activity of chitosans with varying molecular weights,” Food Chemistry, vol. 101, no. 1, pp. 308–313, 2007. View at: Publisher Site | Google Scholar

Copyright © 2019 Dang Xuan Du and Bui Xuan Vuong. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


More related articles

 PDF Download Citation Citation
 Download other formatsMore
 Order printed copiesOrder
Views2740
Downloads761
Citations

Related articles

We are committed to sharing findings related to COVID-19 as quickly as possible. We will be providing unlimited waivers of publication charges for accepted research articles as well as case reports and case series related to COVID-19. Review articles are excluded from this waiver policy. Sign up here as a reviewer to help fast-track new submissions.