In Vitro Characterization of Multilamellar Fibers with Uniaxially Oriented Electrospun Type I Collagen Scaffolds

Figure 3

Scanning electron micrographs of electrospun collagen fibers before and after cross-linking. (a) The orientation of the aligned fibers in each layer compared to the fibers in adjacent lamella. (b) The morphology of the parallel arrangement of collagen fibers in non-cross-linked scaffold. (c) Fiber orientation histogram. (d and e) Cross-linked multilayered scaffolds with aligned uniaxially oriented electrospun fibers in each layer. Black arrow shows the transparency of the scaffold. (f) Freeze fracture of cross-linked multilayered collagen fibers (white arrow).

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