Research Article | Open Access
Determinants of Tuberculosis Infection among Adult HIV Positives Attending Clinical Care in Western Ethiopia: A Case-Control Study
There has been a drastic rise of tuberculosis (TB) infection across the world associated with the pandemic occurrence of HIV/AIDS. There are various determinants factors that increase the chance of TB infection among HIV positives (TB/HIV confection) that varies contextually. This study aimed to assess the determinants of TB/HIV coinfection among adult HIV positives attending clinical care at two public health facilities in Nekemte, western Ethiopia. Unmatched case-control study was conducted from December 26, 2011, to February 29, 2012. Cases were 123 TB infected HIV positives, and controls were 246 non-TB infected HIV positives. Being divorced/widowed , 95% CI (1.70, 7.88), not attending formal education , 95% CI (2.20, 14.15), being underweight ( kg/m2) , 95% CI (2.18, 6.87), having history of diabetic mellitus , 95% CI (1.33, 9.94), and being in advanced WHO HIV/AIDS clinical staging , 95% CI (1.32, 3.98), were determinant factors associated with TB/HIV co-infection. Having a separate kitchen , 95% CI (0.28, 0.81) showed protective role. For most of these determinants interventions can be made at individual and institutional levels, whereas, factors like education and nutrition need societal level integrations.
Tuberculosis (TB) is one of the world’s most common cause of death in the era of human immunodeficiency virus (HIV). It is among the leading causes of death for people living with HIV (PLWHIV) which shares about twenty-five percent of all causes of the deaths . TB and HIV are called a “deadly duo” as HIV weakens the immune system and makes them more susceptible for TB infection. On the other hand, TB increases the progression of HIV to AIDS stage .
Globally, more than 13 million people are TB/HIV coinfected. Of these, about seventy percent are living in sub-Saharan Africa . Ethiopia ranked seventh among the twenty-two high TB burden countries in the world . Hospital data indicated that TB is the leading cause of morbidity and the third cause of hospital admissions in the country .
Studies indicated that certain HIV-infected people develop TB, while others do not. This phenomenon iterates that being HIV positive is not a mere factor for being infected with TB, and there are various determinants factors that contribute to the TB/HIV co-infection [6–8]. These factors vary contextually which necessitate conducting the present study. Therefore, the aim of this study was to identify the determinants of TB/HIV co-infection among HIV-positive adults attending clinical care at two public health facilities in Nekemte, western Ethiopia.
2.1. Study Setting
This study was conducted in one public hospital and a health center found in Nekemte which is the capital town of east Wollega Zone of Oromia Regional State. The town is located at about 331 km from Addis Ababa (the capital city) to the west of country. The two public health facilities are providing free ART services in the town since 2005 and 2006, respectively. In 2010/11, a number of clients attending HIV clinical care were 8,760 PLHIV in the hospital and 1,660 in the health center. Overall, 184 TB/HIV co-infected patients were in clinical care during the commencement of this study (146 in the hospital and 38 in the health center) . This study was conducted between December 26, 2011, and February 29, 2012.
The design for this study was unmatched case control. Cases were TB/HIV co-infected patients whereas controls were non TB infected HIV-positive patients. The inclusion criteria for the study were being 15 years and above, TB/HIV coinfected (PTB, EPTB, mixed or disseminated) for cases and PLHIV with non-TB infected were selected for controls. Those patients who were seriously ill, had a mental problem and unable to give consent, transferred to other facilities outside of the study area, and not confirmed as TB were excluded from the study.
To select the study subjects, lists of cases and controls were prepared using unique identification numbers from records found in ART clinics by computer-generated random numbers. Cases and controls were selected proportional to a number of patients on clinical care in the health facilities during the study period. With the procedure, 123 TB co-infected and 246 non-TB infected HIV positives were selected for cases and controls, respectively.
Being tuberculosis co-infected or not co-infected is the dependent variable for the study. The independent variables include sociodemographic and economic characteristics (age, sex, income, educational status, and marital status), host and clinical related characteristics (WHO clinical stage, CD4 count, past history of TB, body mass index (BMI), smoking, history of asthma, and diabetic mellitus), and environmental related characteristics (overcrowding, wall and floor type of residential house).
2.4. Data Collection
Five trained nurse counsellors (3 for the hospital and 2 for health centres) who were fluent speakers of the local languages (Afan Oromo and Amharic) collected the data. Pretested structured questionnaire was used to collect the data. The data collection was through face-to-face interview approach. The interview was made in a separate room to ensure privacy and to facilitate discussions between the interviewer and the respondent. Other clinical characteristics data were retrieved from each patient record. The overall data collection process was supervised by two supervisors (public health professionals) and the investigators.
2.5. Data Analysis
Data were cleaned and entered to Epi info version 3.5.3 software. Analysis was made using SPSS version 16.0 software package. Descriptive statistics were used to assess normality, outliers and identify missing values. Bivariate analysis was performed to see the association between the dependent and independent variables. To measure the strength of association, odds ratio with a 95% confidence level was calculated. Multivariable logistic regression was done by entering all variables with value less than 0.25 in the bivariate analysis. Finally, unconditional logistic regression with backward method was used to control possible confounders and to identify the determinant factors associated with TB/HIV co-infection.
2.6. Ethical Considerations
The study was reviewed and approved by the Institutional Research Ethics Review Committee of the Colleges of Health and Medical Sciences, Haramaya University. Moreover, written consent was obtained from all study participants before commencement of the study.
3.1. Sociodemographic Characteristics
A total of 357 (119 cases and 238 controls) were included in the study. The response rate was 96.7%. More than half 192 (53.8%) of them were females. The median (IQR) age was 35 years (IQR = 10). Three hundred twenty-seven (91.6%) were urban dwellers and 251 (70.3%) attended formal education. In bivariate analysis, those widowed/divorced were 2.7 times more likely to develop TB than those who were single or married, OR = 2.7, 95% CI (1.49, 5.34). Those who had not attended formal education were 4.5 times more likely to develop TB than those who attended tertiary level education, OR = 4.55, 95% CI (2.09, 9.90) (Table 1).
**1 USD = 18.5 ETB in 2012.
3.2. Host and Clinical Related Characteristics
Sixteen of the cases (13.4%) and 10 (4.2%) of the controls had been experienced diabetic mellitus. Those with history of diabetic mellitus were more likely infected with TB, OR = 3.53, 95% CI (1.55, 8.07) though did not reach significance after controlling for the confounders. Low CD4 cell count (<200 cell/mm3) was significantly associated with TB infection, OR = 2.35, 95% CI (1.23, 4.48). Compared to the respondent who had CD4 count greater than 500 cells/mm3, those who had CD4 count below 200 and 200–500 cell/mm3 were 2.3 and 1.4 times more likely to develop TB infection, respectively. Those who were in WHO clinical stages three and four were two times more likely to develop TB than those in stage one and two OR = 2.09, 95% CI (1.33, 3.28). Those who had hemoglobin level of less than 10 mg/dL, OR = 2.96, 95% CI (1.28, 6.80), were more likely to develop tuberculosis than those who had 12.5 mg/dL and above hemoglobin level. Having BMI less than 18.5 m2/kg was associated with TB infection, OR = 3.80, 95% CI (2.39, 6.08).
3.3. Environmental Related Characteristics
Eighty (67.2%) of the cases and 179 (75.2%) of the controls had less than five adults in the house hold. Higher proportion of the controls 213 (89.5%) had disposed waste outside of the compound when compared to the cases. Ninety-one (76.5%) of the cases and 143 (60.1%) of the controls house floor were made of soil. Having house made of soil floor was significantly associated with TB () though did not reach statistically significant after controlling for confounders. Forty-seven (39.5%) of the cases and 60 (25.2%) of the controls did not have separated kitchen. The presence of separate kitchen decreased the chance of acquiring TB by 48%, OR = 0.52, 95% CI (0.32–0.82) (Table 4).
3.4. Factors Independently Associated with TB/HIV Coinfection
Factors independently associated with TBHIV co-infection were having no formal education, divorced/widowed, underweight ( kg/m2), having history of diabetic mellitus, being in an advanced WHO clinical stage of HIV/AIDS. Having a separate kitchen was negatively associated with TB/HIV co-infection (Table 5).
In this study, being divorced or widowed, not attending formal education, being underweight, having history of diabetic mellitus, and being in advanced WHO clinical stage of HIV were factors associated with TB infection. Very importantly, having a separate kitchen from residential house had a protective effect from acquiring TB infection.
We had obtained consistent finding with previous studies from Gambia and Guinea Bissau [10, 11] where patients who were divorced/widowed were at greater risk of TB/HIV co-infection. This can be seen in the view of marriage having a positive effect on health of an individual in a sense that those who get married and stayed together have advantages of better health as a result of positive psychological and social impacts . Furthermore, evidence has shown that tuberculosis can lead to marriage disruption  so that they could be in divorced/widowed state during the study time which is difficult to ascertain by this study and left exposed for further research.
Individuals who attended formal education are more likely efficient producers of health as they have higher awareness and can take precautionary measures to prevent health problems. We identified that those who have no formal education were more likely acquire TB infection than those who attended formal education. Studies from India, Gambia and Jimma (Ethiopia) also reported similar findings [6, 10, 14, 15].
Previous research indicated that low body mass index which explains undernutrition is significantly associated with TB infection [16, 17]. This might be due to the fact that undernutrition weakens the immunity level that increases the reactivation of latent tuberculosis infection. Building on the existing knowledge this study also found out that low body mass index increased the likelihood tuberculosis co-infection.
The other important finding identified was patients WHO clinical staging and TB-coinfection association. Those in third and fourth WHO clinical stages were about two times more likely to develop TB compared with those in WHO clinical stages one and two. Congruent findings have been reported before from in-country and outside of the country [6, 7, 18, 19]. This could be explained as once the patients get into late stages, the immunity protective capacity will be minimal which would make them prone to tuberculosis infection. A worth to mention as well is that TB is one of the ADIS defining criteria to categorize the patients in to the late WHO clinical staging which again was not captured with this study.
Furthermore, having known history of diabetic mellitus was 3.6 times more likely associated with TB/HIV co-infection as compared to those who did not have known history of diabetic mellitus. Likewise, different studies reported similar findings [15, 20, 21]. This can be explained as diabetes mellitus-related complications increase the acquisition of TB and for people with both diseases the TB treatment is more ineffective .
Interestingly, this study also showed that having a separate kitchen decreased the risk of TB co infection by 52% compared with those who did not have. Similar findings had been reported from south India and in Jimma (Ethiopia) [6, 15]. This might be related with the indoor biomass use that could be associated with air pollution that could contribute to the respiratory infections including tuberculosis [23, 24].
Finally, this study has limitations. First, because of the nature of the study design, and data collection approaches, social desirablity bias is unavoidable. Secondly, recall bias is expected as some of the variables asked retrospectively. Lastly, reverse causal relationship might exist which could have an effect on the generalizability of the findings.
The study came up with that educational and marital status, body mass index, WHO clinical staging, and having history of diabetic mellitus are associated with TB co-infection among adult HIV positives. Most of these factors can be intervened at individual and institutional levels though some like education and nutrition need a broader societal level integration to intervene.
Conflict of Interests
The authors have no conflict of interests.
H. Melkamu, B. Seyoum and Y. Dessie participated in designing study, data collection, statistical analysis, and paper preparation. All the authors read and approved the final paper.
We are grateful to the Haramaya University School of Graduate Study. The authors would also like to acknowledge the study participants, supervisors and data collectors.
- World Health Organization, Tuberculosis Fourth Edition, The Essentials, Edited By Dr Mario C. Raviglione, World Health Organization, Geneva Switzerland, 2010, http://www.who.int/tb/features_archive/the_essentials/en/.
- World Health Organization, Promoting the Implementation of Collaborative TB/HIV Activities through Public-Private Mix and Partnerships Report of a WHO Consultation, World Health Organization, Geneva, Switzerland, 2008, http://whqlibdoc.who.int/hq/2008/WHO_HTM_TB_2008.408_eng.pdf.
- Joint United Nations Programme on HIV/AIDS/World Health Organization, “Nutrition and tuberculosis, a review of the literature and consideration for TB control programs,” 2008, http://works.bepress.com/ppapatha/2/.
- World Health Organization, WHO Country Cooperation Strategy 2008–2011, Ethiopia, WHO Regional Office for Africa, Brazzaville, Congo, 2009, http://www.afro.who.int/.
- Federal Ministry of Health Ethiopia, Tuberculosis, Leprosy and TB/HIV Prevention and Control Programme Manual, Fourth Edition, MOH, Addis Ababa, Ethiopia, 2008.
- M. Taha, A. Deribew, F. Tessema, S. Assegid, L. Duchateau, and R. Colebunders, “Risk factors of active tuberculosis in people living with HIV/AIDS in southwest Ethiopia: a case control study, in Jimma Hospital and Karl Hospital in Southwest Ethiopia,” Ethiopian Journal of Health Development, vol. 219, no. 2, pp. 131–139, 2009.
- B. J. Ngowi, S. G. Mfinanga, J. N. Bruun, and O. Morkve, “Pulmonary tuberculosis among people living with HIV/AIDS attending care and treatment in rural northern Tanzania,” BMC Public Health, vol. 8, no. 341, 2008.
- W. Were, D. Moore, P. Ekwaru et al., “A simple screening tool for active tuberculosis in HIV-infected adults receiving antiretroviral treatment in Uganda,” International Journal of Tuberculosis and Lung Disease, vol. 13, no. 1, pp. 47–53, 2009.
- Nekemte Town Health Office, Annual Health Report Nekemte, Western Ethiopia, Nekemte Town Health Office, Nekemte, Ethiopia, 2011.
- P. C. Hill, D. Jackson-Sillah, S. A. Donkor, J. Otu, R. A. Adegbola, and C. Lienhardt, “Risk factors for pulmonary tuberculosis: a clinic-based case control study in The Gambia,” BMC Public Health, vol. 6, no. 156, 2006.
- C. Lienhardt, K. Fielding, J. S. Sillah et al., “Investigation of the risk factors for tuberculosis: a case-control study in three countries in West Africa,” International Journal of Epidemiology, vol. 34, no. 4, pp. 914–923, 2005.
- M. Gallagher, “Marriage and Public Health: Institute for American Values The Case for Marriage,” 2001, USA, http://www.americanvalues.org/.
- A. Courtwright and A. N. Turner, “Tuberculosis and stigmatization: pathways and interventions,” Public Health Reports, vol. 125, no. 4, pp. 34–42, 2010.
- A. Kaulagekar and A. Radkar, “Social status makes a difference: tuberculosis scenario during National Family Health Survey-2,” The Indian Journal of Tuberculosis, vol. 54, no. 1, pp. 17–23, 2007.
- N. Shetty, M. Shemko, M. Vaz, and G. D'Souza, “An epidemiological evaluation of risk factors for tuberculosis in South India: a matched case control study,” International Journal of Tuberculosis and Lung Disease, vol. 10, no. 1, pp. 80–86, 2006.
- E. Mupere, S. Zalwango, A. Chiunda, A. Okwera, R. Mugerwa, and C. Whalen, “Body composition among HIV-seropositive and HIV-seronegative adult patients with pulmonary tuberculosis in Uganda,” Annals of Epidemiology, vol. 20, no. 3, pp. 210–216, 2010.
- C. F. Hanrahan, J. E. Golub, L. Mohapi et al., “Body mass index and risk of tuberculosis and death,” AIDS, vol. 24, no. 10, pp. 1501–1508, 2010.
- R. Teck, O. Ascurra, P. Gomani et al., “WHO clinical staging of HIV infection and disease, tuberculosis and eligibility for antiretroviral treatment relationship to CD4 lymphocyte counts,” International Journal of Tuberculosis and Lung Disease, vol. 9, no. 3, pp. 258–262, 2005.
- S. D. Lawn, M. Badri, and R. Wood, “Tuberculosis among HIV-infected patients receiving HAART: long term incidence and risk factors in a South African cohort,” AIDS, vol. 19, no. 18, pp. 2109–2116, 2005.
- R. Coker, M. McKee, R. Atun et al., “Risk factors for pulmonary tuberculosis in Russia: case-control study,” British Medical Journal, vol. 332, no. 7533, pp. 85–87, 2006.
- Ch. Dye, B. B. Trunz, K. Lönnroth, G. Roglic, and B. G. Williams, “Nutrition, diabetes and tuberculosis in the epidemiological transition,” PLoS ONE, vol. 6, no. 6, Article ID e21161, 2011.
- World Diabetes Foundation, International Diabetes Federation, Novo Nordisk, and Diabetes South Africa, “Diabetes:the hidden pandemic and its impact on sub-Saharan Africa,” Diabetic Leadership Forum Africa, 2010.
- P. V. M. Lakshmi, N. K. Virdi, J. S. Thakur, K. R. Smith, M. N. Bates, and R. Kumar, “Biomass fuel and risk of tuberculosis: a caseecontrol study from Northern India,” Journal of Epidemiology & Community Health, vol. 66, no. 5, pp. 457–461, 2010.
- X. Kan, C. Chiang, D. A. Enarson, W. Chen, J. Yang, and G. Chen, “Indoor solid fuel use and tuberculosis in China: a matched case-control study,” BMC Public Health, vol. 11, no. 498, 2011.
Copyright © 2013 Hatoluf Melkamu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.