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Journal of Biomedicine and Biotechnology
Volume 2009, Article ID 104853, 6 pages
http://dx.doi.org/10.1155/2009/104853
Research Article

Combined Use of MS2 and PP7 Coat Fusions Shows that TIA-1 Dominates hnRNP A1 for K-SAM Exon Splicing Control

1INSERM, U892, 8 quai Moncousu, BP 70721, 44007 Nantes Cedex 1, France
2Faculté des Sciences, Université de Nantes, 44300 Nantes, France

Received 17 July 2009; Accepted 30 October 2009

Academic Editor: Patrick Matthias

Copyright © 2009 Marie-Claude Gesnel et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Splicing of the FGFR2 K-SAM exon is repressed by hnRNP A1 bound to the exon and activated by TIA-1 bound to the downstream intron. Both proteins are expressed similarly by cells whether they splice the exon or not, so it is important to know which one is dominant. To answer this question, we used bacteriophage PP7 and bacteriophage MS2 coat fusions to tether hnRNP A1 and TIA-1 to distinct sites on the same pre-mRNA molecule. hnRNP A1 fused to one coat protein was tethered to a K-SAM exon containing the corresponding coat protein's binding site. TIA-1 fused to the other coat protein was tethered to the downstream intron containing that coat protein's binding site. This led to efficient K-SAM exon splicing. Our results show that TIA-1 is dominant for K-SAM exon splicing control and validate the combined use of PP7 and MS2 coat proteins for studying posttranscriptional events.