Proteomic Identification of Dengue Virus Binding Proteins in Aedes aegypti Mosquitoes and Aedes albopictus Cells
Identification of enolase, beta-adrenergic receptor kinase, and translation elongation factor EF-1 alpha/Tu as DENV-binding proteins by LC MS/MS analysis of the excised protein bands corresponding to the apparent molecular weights of 57 and 67 kDa. The colored sequences represent the amino acid peptides identified as enolase (a), beta-ARK (b), and translation elongation factor EF-1 alpha/Tu (c) using MS/MS spectrometry after in-gel digestion of the protein-staining band (Tables 1 and 2). The protein sequence refers to gi|157121051|ref|XP_001653750|, gi|157114479|ref|XP_001652291|, and gi|94468780|gb|ABF18239.1|, respectively.
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