Bisphenol A Is More Potent than Phthalate Metabolites in Reducing Pancreatic β-Cell Function

Figure 5

No effect of environmental chemicals on cytokine-induced cell death. (a) INS-1E cells were incubated with the indicated concentrations of environmental chemicals for 72 h with exposure to the proinflammatory cytokines IL-1β (5 ng/mL), TNFα (25 ng/mL), and INFγ (25 ng/mL) the last 48 h of exposure. In the combinatory exposures the indicated doses reflect the concentrations of each chemical rather than the total (additive) chemical concentration. The cell viability was measured by the MTT assay and normalized to control, for example, divided by the mean of all the controls in that experiment. The symbols indicate significant decreases compared to controls or to the same concentrations of # MnBP, MiBP, ¤  MEHP, and ~ combinatory exposure to MnBP, MiBP, and MEHP (two-way ANOVA with Bonferroni posttest, ). (b) Comparison of the effects of environmental chemicals in absence and presence of the proinflammatory cytokines. The figure shows data from Figures 1(a) and 5(a) relative to their respective controls. There were no significant differences between the data obtained in absence or presence of cytokines (two-way ANOVA with Bonferroni posttest, ).

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