Increased Temperature Facilitates Adeno-Associated Virus Vector Transduction of Colorectal Cancer Cell Lines in a Manner Dependent on Heat Shock Protein Signature

Table 1

Summary of hyperthermia influence on rAAV transduction efficiency of CRC and colon fibroblast cells.

CRC lines			RKO	HT-29	LS411N	CCD-18Co
Hyperthermia conditions (43°C, 1 h)	rAAV transduction efficiency		40%	30%	10%	34%
	Increase of rAAV receptors expression	AAVR	↑↑↑	↑↑	↑↑	↑↑
		HSPG1	-	-	-	↑↑
		HSPG2	↑↑	↑↑	↑	↑↑
	Increase of HSP expression	DNAJC4	↑↑↑↑	↑↑
		HSP90AB1		↑↑
		HSPA2		↑↑	↑↑↑↑	↑↑
		HSPA5	↑↑	↑↑↑		↑↑
		HSPA6			↑↑↑↑
		HSPA8	↑↑			↑↑
		HSPA9		↑↑↑
		HSPB1	↑↑			↑↑↑↑
		HSF4		↑↑↑↑

The table collects the key results from Figures 7 and 9. The levels of upregulated genes after exposed to hyperthermia were marked as arrows. Expression of rAAV receptors: ↑ 0.5–1.0; ↑↑ 1.01–1.5; ↑↑↑ 1.51–2.0; and expression of HSP: ↑ 0.95–1.05; ↑↑ 1.051–1.15; ↑↑↑ 1.151–1.20; ↑↑↑↑ 1.25–3.25. The analyzed results were expressed in the form of 2^−ΔΔCt (expression fold change).