Project-Based Learning to Promote Effective Learning in Biotechnology Courses

Table 1

Scheduled labs in Bio-251.

Week	Scheduled labs
1	Basic laboratory safety techniques; Basic equipment and bacteriological techniques
2	Basic calculations for preparing solutions of different concentrations
3	Bacterial media prep, inoculation, and growth
4	Isolation of chromosomal DNA and preparation of agarose gel
5	PCR and agarose gel analysis
6	Restriction enzyme digestion, purification, and preparation for cloning
7	Ligation
8	Transformation; Making competent cells of E. coli and transforming the recombinant plasmid.
9	Isolation of Plasmid, gel electrophoresis, and screening for clone
10	Preparation for gene expression and transformation to a suitable strain for expression
11	Protein induction and expression
12	Polyacrylamide gel electrophoresis
13	Protein purification
14	Protein purification troubleshooting
15	Bioinformatics (genome analysis, Blast, Primer3 for designing PCR, Web-cutter)
16	Computer analysis of the gene product