Cell Adhesion in CancerView this Special Issue
Review Article | Open Access
Makoto Nagano, Daisuke Hoshino, Naohiko Koshikawa, Toshifumi Akizawa, Motoharu Seiki, "Turnover of Focal Adhesions and Cancer Cell Migration", International Journal of Cell Biology, vol. 2012, Article ID 310616, 10 pages, 2012. https://doi.org/10.1155/2012/310616
Turnover of Focal Adhesions and Cancer Cell Migration
Cells are usually surrounded by the extracellular matrix (ECM), and adhesion of the cells to the ECM is a key step in their migration through tissues. Integrins are important receptors for the ECM and form structures called focal adhesions (FAs). Formation and disassembly of FAs are regulated dynamically during cell migration. Adhesion to the ECM has been studied mainly using cells cultured on an ECM-coated substratum, where the rate of cell migration is determined by the turnover of FAs. However, the molecular events underlying the disassembly of FAs are less well understood. We have recently identified both a new regulator of this disassembly process and its interaction partners. Here, we summarize our understanding of FA disassembly by focusing on the proteins implicated in this process.
Adhesion of cells to the ECM is key to the regulation of cellular morphology, migration, proliferation, survival, and differentiation [1, 2]. These functions are indispensable during development and for maintenance of tissue architecture and the induction of tissue repair. Integrins are the predominant receptors that mediate cell adhesion to components of the ECM [3–8]. Integrins are expressed on the cell surface as heterodimers comprised of noncovalently associated α- and β-subunits. Both subunits are type I transmembrane proteins containing both a large extracellular domain responsible for binding to ECM ligands and a cytoplasmic portion (CP) that recruits multiple intracellular proteins. Eighteen different α- and 8 β-subunits have been characterized in mammals, and 24 distinct integrin heterodimers have so far been identified [5, 9, 10]. Each integrin recognizes a distinct ECM ligand. As such, the repertoire of integrins expressed on the surface of a particular cell acts as a sensor of the ECM environment .
Attachment of cells to ECM components induces clustering of integrins on the cell surface. The cytoplasmic portions of the clustered integrins then act as a platform for the recruitment of cellular proteins such as adaptor/scaffold and signaling proteins to the inner surface of the plasma membrane, where they form structures called focal adhesions (FAs) (Figure 1) [11–13]. The adaptor/scaffold proteins in FAs, such as talin, paxillin, tensin, p130Cas, and α-actinin, provide strong linkages to the actin cytoskeleton and, thereby, connect cells firmly to the ECM [14–18]. This linkage enables the generation of the tension necessary to alter cell morphology and the traction force necessary to move the cell body during migration. In addition, multiple signaling proteins, including kinases or phosphatases, are also recruited to FAs where they transmit ECM-derived signals to cellular pathways controlling proliferation, survival and migration [19–23]. In particular, two well-characterized tyrosine kinases, focal adhesion kinase (FAK) and Src, play central roles in integrin-mediated signaling cascades [20, 24, 25]. Since integrins have no intrinsic enzymatic activity, these tyrosine kinases transmit signals from FAs to the cellular machinery by phosphorylating multiple integrin-associated proteins [25–30]. Thus, both FAK and Src act as molecular switches that trigger a variety of cellular responses via FA complexes. There are many excellent reviews discussing how integrin-mediated signals regulate cellular behavior [20, 25, 31, 32].
The process of cell adhesion to the ECM has been studied by seeding cells onto an ECM-coated substratum in culture [33, 34]. These analyses contributed to the elucidation of the process of the initial attachment of cells to the ECM and the formation of integrin-mediated cell adhesion structures. However, cells must also detach from the ECM during migration, and the mechanism and regulation of the disassembly of cell adhesion structures is less well studied. In contrast to most review articles discussing cell adhesion, we focus here on our understanding of the turnover of FA complexes during cell migration.
2. Adhesion of Cells through the Formation of Focal Adhesion Structures
Cells adhere to the ECM via integrins and form FA complexes as discussed elsewhere . Numerous proteins are involved in integrin-mediated cell adhesion, and these proteins are collectively referred to as the adhesome [35–38]. Among the latter, talin is a key regulator of the initial step of FA assembly [39–41]. Talin contains two unique domain structures, the head and rod domains [42–45]. The head domain mediates binding to the CP of the β-subunit of integrin, whereas the rod domain contains multiple binding sites for adhesome proteins, including one for the CP of β-integrin, two sites for actin, and multiple sites for vinculin. In addition, talin forms a dimer through its carboxy-terminal helix and thus serves as a core platform to expand intracellular structural frameworks mediated by protein-protein interactions. The binding of talin to integrin stabilizes the ligand-induced clustering of the latter at an initial step of FA formation by mediating crosslinking of integrins with filamentous actin (F-actin) and F-actin-binding proteins such as vinculin and α-actinin (Figure 3(a)) [14, 46–48]. This initial structure, called the nascent FA, is immature and often short lived . However, some of the nascent FAs grow and form mature FAs that require actin-based tension regulated by the Rho small GTPase and its effector ROCK .
3. Regulation of Focal Adhesion Complexes during Cell Migration
Stimulation of the formation of FA complexes enhances the adhesion of cells to the ECM, giving rise to cells with a spread morphology (Figure 2(i)). In contrast, destabilization of FAs reduces adhesion to the ECM and gives rise to spherical nonadherent cells (Figure 2(ii)). During cell migration on a substratum, FAs grasp the ECM so as to generate the forces necessary to pull the cell body forward. Subsequently, cells must release from the ECM, so as to continue cell movement. As such, directional migration of the cell requires continuous, coordinated formation and turnover of FAs at the leading edge of the cell body and release of this attachment at the rear (Figure 2(iii)) [49, 50]. Clustering of integrins is the initial step of cell adhesion and is stabilized to form FAs by linking to actin stress fibers in a process regulated by Rho/ROCK [6, 51, 52]. By contrast, extension of microtubules to FAs triggers their disassembly and induces the subsequent internalization of integrins from the cell surface [53–56]. Therefore, the assembly and disassembly of FAs are regulated by different mechanisms. Although the fate of the internalized integrins has not yet been established, several studies have reported the transport of internalized integrins from the rear to the leading edge of the cell body via intracellular vesicle trafficking [57–59]. This recycling of integrins may contribute to directional cell migration.
4. Factors Involved in the Disassembly of FAs
The molecular events leading to FA disassembly are not yet well understood although some fragmentary knowledge has recently accumulated [54, 60, 61]. Most importantly, it has been established that microtubules (MTs) play a crucial role in inducing FA disassembly . MTs extend to FAs and trigger the disassembly process. During the final stage, the internalization of integrins is mediated by dynamin, a GTPase that regulates endocytosis, and FAK is involved in the recruitment of dynamin into FAs (summarized in Figure 3(b)).
In the following sections, we summarize the proteins involved in disassembly and link their involvement in this process so as to generate a more coordinated model of disassembly based on recent findings. Various disassembly factors and their domain structures are schematically illustrated in Figure 4.
The importance of MTs for FA disassembly has been demonstrated using nocodazole, which disrupts polymerized MTs in cells adherent to the ECM [54, 56]. Exposure of cells to nocodazole stabilizes FA structures by preventing their disassembly and thereby enhances adhesion of cells to the ECM. The removal of the drug from the culture media initiates disassembly of FAs in a synchronous manner and recovery of MT structures [53, 54, 62]. Thus, the use of this drug allows us to analyze the FA disassembly process independently from FA formation. Tyrosine phosphorylation of proteins within FAs increases following exposure to nocodazole and decreases rapidly after its removal. Extension of MTs to FAs has been observed by live imaging microscopy, and targeting of MTs to FAs appears to trigger FA disassembly . Since the MT motor protein, kinesin-1, has been implicated in regulating MT-induced FA disassembly , MTs may deliver disassembly factors to FAs in a kinesin-1-dependent fashion.
As extension of MTs to FAs triggers release of cell adhesion and promotes cell migration, it is of interest how targeting of MTs to FAs is regulated during the induction of cell motility. Indeed, Rho family GTPases regulates the capture and stabilization of extended MTs to the cell cortex via their downstream effectors, and MTs in turn have been shown to affect the activity of Rho GTPases . Although it is not precisely clear how MTs target FAs, actin filaments presumably play a role.
Kinesin-1 is a member of the kinesin superfamily of motor proteins and is also known as conventional kinesin [64, 65]. Kinesin-1 plays a crucial role in protein trafficking along polymerized MTs to the direction of plus end of latter. The inhibition of kinesin-1 in Xenopus fibroblasts, using either a specific antibody or forced expression of a dominant-negative mutant, leads to stabilization of FAs accompanied by an increase in their size and a reduction in their number, as was seen in cells exposed to nocodazole . These findings suggest that kinesin-1 activity is necessary for the turnover of FAs. Although nocodazole inhibits the polymerization of MTs, inhibition of kinesin-1 activity affects neither the targeting of MTs to FAs nor the polymerization dynamics of MTs . This suggests that FA disassembly factors are conveyed along MTs in a kinesin-1-dependent manner.
4.3. Focal Adhesion Kinase
FAK is involved in both maturation and turnover of FAs [20, 66]. However, FAK deficiency has a greater effect upon disassembly than upon formation of FAs, giving rise to a reduced rate of FA turnover leading to an increase in the level of steady-state FAs [60, 66]. FAK contains an N-terminal FERM (protein 4.1, ezrin, radixin, and moesin homology) domain, a central kinase domain, and a COOH-terminal focal adhesion-targeting (FAT) domain as illustrated in Figure 4. The FERM domain is found in many proteins and mediates protein-protein interactions [67, 68]. The FAK FERM domain has been shown to bind the CP of integrin β1 and growth factor receptors [69, 70]. Recent structure analysis of the FERM domain has indicated that it binds the catalytic cleft of the kinase domain . This intramolecular interaction prevents autophosphorylation of Tyr397, which is a prerequisite for the successive phosphorylation of FAK by Src. Autophosphorylation of FAK at Tyr397 is elevated in highly motile and invasive cancer cells [72, 73]. Src binds to phosphorylated Tyr397 and further phosphorylates multiple tyrosine residues within FAK, including Tyr576 and Tyr577 within the kinase domain, Tyr861 located between the kinase and FAT domain, and Tyr925 within the FAT domain [20, 25]. Phosphorylation within the kinase domain is crucial for full kinase activity. Phosphorylated Tyr861 mediates the interaction of FAK with talin and paxillin [20, 25]. Phosphorylation at Tyr925 is necessary for the interaction of FAK with Grb2 [20, 25]. Binding of Grb2 to FAK helps recruit dynamin to FAs . This ternary complex is responsible for the internalization of integrins and thereby induces turnover of FAs. However, the role of FAK during FA disassembly is not so simple. Whereas pTyr397 FAK is required for recruitment of dynamin, its dephosphorylation is induced after extension of MTs to FAs, and this is a prerequisite step for the successive disassembly of FAs [54, 62]. Thus, FAK is a central regulator of the formation and disassembly of FAs, and for the transmission of integrin-mediated signals. Nevertheless, FAK deficiency has little effect upon FA formation but has nevertheless been shown to stabilize FAs. The roles of FAK during FA formation might be performed by other redundant kinases or factors recruited to FAs.
Dynamin is a GTPase that was identified as an MT-binding protein . Three independent dynamin genes have been identified. Dynamin I is expressed specifically in neurons, and Dynamin III is expressed exclusively in testis, lung, and brain, whereas Dynamin II is expressed ubiquitously . The domain structure common to the dynamins is shown in Figure 4. Dynamin is required for the internalization of integrins during MT-dependent FA turnover . The carboxyl terminus of dynamin contains a proline-rich (PR) motif, which is indispensable for assembly of a ternary complex with FAK and Grb2 . The PR motif of dynamin also interacts with MTs. Dynamins recruited to the inner surface of the cells membrane assemble in a ring around FAs  and initiates the internalization of integrins when the FAs are sufficiently disassembled. FAK deficiency markedly reduces the accumulation of dynamin around FAs . Interaction of the tubulin polymer with dynamin markedly increases the GTPase activity of the latter, although the physiological significance of this is unclear .
A specific set of protein tyrosine phosphatases mediates dephosphorylation of FAK at Tyr397 after the extension of MTs to FAs . These include PTP-PEST, SHP-2, and PTP-1B. However, it is not clear whether FA disassembly requires concerted action of all three phosphatases or whether the action of a single phosphatase is sufficient, depending on the cellular context.
PTP-PEST is known to regulate cell adhesion and migration (Figure 4) . As Zheng et al. have reported, PTP-PEST dephosphorylates FAK at Tyr397 upon activation by an oncogenic Ras-induced signal [77, 78]. Ras induces the activation of ERK via the Fgd1-Cdc42-PAK1-MEK1 cascade ultimately resulting in interaction between FAK and PTP-PEST. Activated ERK phosphorylates FAK at Ser910, and the phosphorylated Ser910 and the adjacent Pro911 residue serves as a binding site for peptidyl-prolyl cis/trans isomerase (PIN1). PIN1 stimulates the binding of FAK to PTP-PEST, in a fashion dependent upon the isomerase activity of PIN1, although the exact role of the isomerase activity is not clear. PTP-PEST then dephosphorylates pTyr397 . Intriguingly, substitution of FAK Tyr397 by Phe promotes metastasis of v-H-Ras-transformed rat fibroblasts.
SHP-2 can also dephosphorylate FAK at Tyr397 . SHP-2 contains two SH2 domains at its N-terminus (Figure 4), and the N-terminal most of the two acts as an intramolecular inhibitor of the phosphatase activity . This inhibition can be released by Gab2, a pleckstrin homology (PH) domain-containing docking protein. Gab2 binds the N-terminal SH2 domain and exposes the phosphatase domain of SHP-2 by releasing the intramolecular inhibition . Deficiency of SHP-2 in cultured cells increases the number of FAs and impairs cell migration . These findings are reminiscent of the phenotype of FAK-deficient cells. However, there is no clear evidence that SHP-2 localizes to FAs during their turnover. SHP-2 might be recruited to FAs by interacting with the phosphorylated tyrosines of Gab2 via its two SH2 domains.
There are several substrates for PTP-1B in FAs, including FAK, Src, and α-actinin [83, 84]. PTP-1B is a complicated regulator of FAK. It directly mediates dephosphorylation of pTyr397  but can also promote phosphorylation of the same tyrosine residue by Src . As Zhang reported, α-actinin plays a key role in the dual functions of PTP-1B . α-Actinin phosphorylated at Tyr12 promotes dissociation of Src bound to FAK at pTyr397. This allows PTP-1B to dephosphorylate the exposed pTyr397. On the other hand, PTP-1B can dephosphorylate α-actinin pTyr12 so as to increase the α-actinin-free Src pool that is then available to phosphorylate FAK. At the same time, PTP-1B can activate Src by dephosphorylating Src pTyr527, which mediates intramolecular inhibition of Src activity. Overall, dephosphorylation of FAK by PTP-1B enhances subsequent phosphorylation of FAK by Src. These functions of PTP-1B may play roles in the dynamic turnover of FAs during dynamic cell attachment rather than simply by promoting detachment.
m-Calpain, also known as Calpain-2, is a member of the calpain family of intracellular calcium-dependent proteases . It comprises five functionally and structurally distinct domains. Domain I is a possible autoinhibitory region, and it is cleaved off by autolysis. Domain II is a catalytic domain composed of two split subdomains (IIa and IIb) linked by a loop called the catalytic cleft. Domain III is a putative regulatory region of the protease activity, and it contains phospholipid-binding sites. Domain IV contains four EF-hand motifs that are necessary for binding calcium.
m-Calpain has been shown to regulate the turnover of FAs by cleaving multiple FA-related proteins such as talin, FAK, and paxillin [61, 86–88]. Talin is a well-established substrate of m-Calpain during the turnover of FAs [61, 89]. m-Calpain cleaves a site between the head and the rod domains and thereby triggers structural breakdown of the FA framework . FAK is also cleaved by m-Calpain between the two C-terminal PR domains . Breakdown of FAs by m-Calpain also requires MTs . Even though the precise role of MTs in the breakdown of FAs by m-Calpain is unclear, ZF21 presumably plays a role as explained in next section [62, 91]. FAK can bind both ERK/MAPK and m-Calpain, and it might be a platform where m-Calpain can be activated by the ERK/MAPK . Cleavage of components of FAs by m-Calpain presumably facilitates internalization of integrins by disrupting interconnected large structure of FAs.
ZF21 contains a FYVE domain, which binds to phosphatidylinositol-3-phosphate that is enriched in the lipid layers of plasma membranes. Although there are 38 FYVE domain-containing proteins in mammals, they do not necessarily have common domain structures or functions . ZF21 initially attracted our attention as a possible interaction partner of the cytoplasmic tail of the membrane type metalloproteinase, MT1-MMP, but it was later determined to be a regulator of FA turnover . ZF21 is expressed almost ubiquitously in various types of adhesive cells. The FYVE domain of ZF21 is located in the middle in the protein, and the C-terminal region of the protein contains a novel protein fold that is similar to the PH domain but is lacking the positively charged amino acids necessary to bind phospholipid . Interestingly, ZF21 binds multiple FA disassembly proteins, including FAK, β-tubulin, m-Calpain, and SHP-2 [62, 91, 94]. The FYVE domain of ZF21 binds FAK, and the PH-like domain binds β-tubulin. Almost the entire ZF21 polypeptide chain is required for binding m-Calpain and SHP-2. Substitution of the FYVE domain of ZF21 with a corresponding domain derived from EEA1, another member of the FYVE domain-containing proteins, abolishes its ability to bind FAK and abrogates its ability to mediate MT-induced FA disassembly .
Knockdown of ZF21 expression in cells prevents MT-induced FA disassembly, as well as disassembly-related events, such as dephosphorylation of FAK at pTyr397 and internalization of integrins . Binding of ZF21 to FAK is important for the regulation of FA disassembly because substitution of the FYVE domain with that of EEA1 abolishes both FAK binding and FA disassembly . The PH-like domain is also indispensable for the activity of ZF21 . Taken together, these findings suggest that ZF21 associates with endosomal vesicles moving on MTs via an interaction between the FYVE domain and phosphatidylinositol-3-phosphate within the vesicle membrane. The PH-like domain, which mediates an interaction with β-tubulin, may help stabilize the interaction of ZF21 with MTs and then ride on vesicles. The ability of ZF21 to bind SHP-2 and m-Calpain may facilitate the transport of the latter to FAs via vesicles loaded onto MTs (Figure 5). Upon targeting of MTs to FAs, ZF21 may be transferred to FAs since it can bind FAK, and the ZF21 transferred to the FAs may subsequently anchor the MTs to the FAs. Gab2 in FAs may facilitate the dephosphorylation of FAK by SHP-2 carried in on the MTs. These events are presumably followed by breakdown of FA components by the proteolytic activity of m-Calpain.
Importance of ZF21 for cell migration gave us a clue to understand its role in FA turnover . Knockdown of ZF21 expression by shRNA in cancer cells induced cell spreading on the ECM and suppressed cell migration. Integrin-mediated cell adhesion and migration are important during cancer cell invasion and metastasis although FA-like structures are not obviously recognizable in most cells surrounded by ECM. Indeed, knockdown of ZF21 expression in human mammary carcinoma MDA-MB231 cells suppresses metastatic colony formation in the lung following injection of the cells into the tail vein of mice . However, it is possible that ZF21 regulates metastasis of cancer cells by mechanisms distinct from the regulation of the turnover of FAs.
Our understanding of the mechanism of FA turnover remains fragmentary. However, the mechanisms governing the migration of cells due to regulated adhesion are crucial to the understanding of cancer cell invasion and metastasis. Turnover of FAs is initiated by the extension of MTs to FAs and is completed by the internalization of integrins from the cell surface. Several factors have been implicated in the process of FA disassembly. In particular, the recently identified ZF21 has shed light on this process, owing to its ability to bind multiple proteins involved in FA disassembly. It is of note that FAs are not observed in cells cultured in a collagen lattice, indicating that the presence of integrin-based cell adhesion structures is dependent upon whether the cells are adhering to a rigid surface (2D) or are embedded within a 3-dimensional ECM (3D) [95, 96]. Advanced imaging technologies are powerful tools to elucidate the dynamic roles of FA disassembly factors during cell migration and invasion.
- B. M. Gumbiner, “Cell adhesion: the molecular basis of tissue architecture and morphogenesis,” Cell, vol. 84, no. 3, pp. 345–357, 1996.
- R. O. Hynes and A. D. Lander, “Contact and adhesive specificities in the associations, migrations, and targeting of cells and axons,” Cell, vol. 68, no. 2, pp. 303–322, 1992.
- C. M. Meighan and J. E. Schwarzbauer, “Temporal and spatial regulation of integrins during development,” Current Opinion in Cell Biology, vol. 20, no. 5, pp. 520–524, 2008.
- H. Truong and E. H. J. Danen, “Integrin switching modulates adhesion dynamics and cell migration,” Cell Adhesion and Migration, vol. 3, no. 2, pp. 179–181, 2009.
- R. O. Hynes, “Integrins: bidirectional, allosteric signaling machines,” Cell, vol. 110, no. 6, pp. 673–687, 2002.
- J. T. Parsons, A. R. Horwitz, and M. A. Schwartz, “Cell adhesion: integrating cytoskeletal dynamics and cellular tension,” Nature Reviews Molecular Cell Biology, vol. 11, no. 9, pp. 633–643, 2010.
- A. L. Berrier and K. M. Yamada, “Cell-matrix adhesion,” Journal of Cellular Physiology, vol. 213, no. 3, pp. 565–573, 2007.
- K. H. Martin, J. K. Slack, S. A. Boerner, C. C. Martin, and J. T. Parsons, “Integrin connections map: to infinity and beyond,” Science, vol. 296, no. 5573, pp. 1652–1653, 2002.
- A. Van der Flier and A. Sonnenberg, “Function and interactions of integrins,” Cell and Tissue Research, vol. 305, no. 3, pp. 285–298, 2001.
- B. H. Luo, C. V. Carman, and T. A. Springer, “Structural basis of integrin regulation and signaling,” Annual Review of Immunology, vol. 25, pp. 619–647, 2007.
- C. G. Galbraith and M. P. Sheetz, “Forces on adhesive contacts affect cell function,” Current Opinion in Cell Biology, vol. 10, no. 5, pp. 566–571, 1998.
- S. Liu, D. A. Calderwood, and M. H. Ginsberg, “Integrin cytoplasmic domain-binding proteins,” Journal of Cell Science, vol. 113, part 20, pp. 3563–3571, 2000.
- V. Petit and J. P. Thiery, “Focal adhesions: structure and dynamics,” Biology of the Cell, vol. 92, no. 7, pp. 477–494, 2000.
- J. D. Humphries, P. Wang, C. Streuli, B. Geiger, M. J. Humphries, and C. Ballestrem, “Vinculin controls focal adhesion formation by direct interactions with talin and actin,” Journal of Cell Biology, vol. 179, no. 5, pp. 1043–1057, 2007.
- A. Horwitz, K. Duggan, and C. Buck, “Interaction of plasma membrane fibronectin receptor with talin—a transmembrane linkage,” Nature, vol. 320, no. 6062, pp. 531–533, 1986.
- U. Gluck and A. Ben-Ze'ev, “Modulation of α-actinin levels affects cell motility and confers tumorigenicity on 3T3 cells,” Journal of Cell Science, vol. 107, part 7, pp. 1773–1782, 1994.
- B. Geiger, A. Bershadsky, R. Pankov, and K. M. Yamada, “Transmembrane extracellular matrix-cytoskeleton crosstalk,” Nature Reviews Molecular Cell Biology, vol. 2, no. 11, pp. 793–805, 2001.
- K. Burridge, C. E. Turner, and L. H. Romer, “Tyrosine phosphorylation of paxillin and pp125(FAK) accompanies cell adhesion to extracellular matrix: a role in cytoskeletal assembly,” Journal of Cell Biology, vol. 119, no. 4, pp. 893–903, 1992.
- J. T. Parsons and S. J. Parsons, “Src family protein tyrosine kinases: cooperating with growth factor and adhesion signaling pathways,” Current Opinion in Cell Biology, vol. 9, no. 2, pp. 187–192, 1997.
- S. K. Mitra, D. A. Hanson, and D. D. Schlaepfer, “Focal adhesion kinase: in command and control of cell motility,” Nature Reviews Molecular Cell Biology, vol. 6, no. 1, pp. 56–68, 2005.
- S. Ishibe, D. Joly, X. Zhu, and L. G. Cantley, “Phosphorylation-dependent paxillin-ERK association mediates hepatocyte growth factor-stimulated epithelial morphogenesis,” Molecular Cell, vol. 12, no. 5, pp. 1275–1285, 2003.
- P. C. McDonald, A. B. Fielding, and S. Dedhar, “Integrin-linked kinase—essential roles in physiology and cancer biology,” Journal of Cell Science, vol. 121, no. 19, pp. 3121–3132, 2008.
- A. W. Stoker, “Protein tyrosine phosphatases and signalling,” Journal of Endocrinology, vol. 185, no. 1, pp. 19–33, 2005.
- M. C. Frame, V. J. Fincham, N. O. Carragher, and J. A. Wyke, “v-Src's hold over actin and cell adhesions,” Nature Reviews Molecular Cell Biology, vol. 3, no. 4, pp. 233–245, 2002.
- S. K. Mitra and D. D. Schlaepfer, “Integrin-regulated FAK-Src signaling in normal and cancer cells,” Current Opinion in Cell Biology, vol. 18, no. 5, pp. 516–523, 2006.
- J. Zhai, H. Lin, Z. Nie et al., “Direct interaction of focal adhesion kinase with p190RhoGEF,” Journal of Biological Chemistry, vol. 278, no. 27, pp. 24865–24873, 2003.
- M. B. Calalb, T. R. Polte, and S. K. Hanks, “Tyrosine phosphorylation of focal adhesion kinase at sites in the catalytic domain regulates kinase activity: a role for Src family kinases,” Molecular and Cellular Biology, vol. 15, no. 2, pp. 954–963, 1995.
- S. L. Bellis, J. T. Miller, and C. E. Turner, “Characterization of tyrosine phosphorylation of paxillin in vitro by focal adhesion kinase,” Journal of Biological Chemistry, vol. 270, no. 29, pp. 17437–17441, 1995.
- L. A. Cary, D. C. Han, T. R. Polte, S. K. Hanks, and J. L. Guan, “Identification of p130(Cas) as a mediator of focal adhesion kinase- promoted cell migration,” Journal of Cell Biology, vol. 140, no. 1, pp. 211–221, 1998.
- S. H. Lo, “Focal adhesions: what's new inside,” Developmental Biology, vol. 294, no. 2, pp. 280–291, 2006.
- A. Tomar and D. D. Schlaepfer, “Focal adhesion kinase: switching between GAPs and GEFs in the regulation of cell motility,” Current Opinion in Cell Biology, vol. 21, no. 5, pp. 676–683, 2009.
- M. D. Schaller, “Cellular functions of FAK kinases: insight into molecular mechanisms and novel functions,” Journal of Cell Science, vol. 123, no. 7, pp. 1007–1013, 2010.
- I. Vlodavsky, G. M. Lui, and D. Gospodarowicz, “Morphological appearance, growth behavior and migratory activity of human tumor cells maintained on extracellular matrix versus plastic,” Cell, vol. 19, no. 3, pp. 607–616, 1980.
- I. Vlodavsky and D. Gospodarowicz, “Respective roles of laminin and fibronectin in adhesion of human carcinoma and sarcoma cells,” Nature, vol. 289, no. 5795, pp. 304–306, 1981.
- C. A. Whittaker, K. F. Bergeron, J. Whittle, B. P. Brandhorst, R. D. Burke, and R. O. Hynes, “The echinoderm adhesome,” Developmental Biology, vol. 300, no. 1, pp. 252–266, 2006.
- R. Zaidel-Bar, S. Itzkovitz, A. Ma'ayan, R. Iyengar, and B. Geiger, “Functional atlas of the integrin adhesome,” Nature Cell Biology, vol. 9, no. 8, pp. 858–867, 2007.
- R. Zaidel-Bar, “Evolution of complexity in the integrin adhesome,” Journal of Cell Biology, vol. 186, no. 3, pp. 317–321, 2009.
- R. Zaidel-Bar and B. Geiger, “The switchable integrin adhesome,” Journal of Cell Science, vol. 123, no. 9, pp. 1385–1388, 2010.
- E. Goksoy, Y. Q. Ma, X. Wang et al., “Structural basis for the autoinhibition of talin in regulating integrin activation,” Molecular Cell, vol. 31, no. 1, pp. 124–133, 2008.
- A. Nayal, D. J. Webb, and A. F. Horwitz, “Talin: an emerging focal point of adhesion dynamics,” Current Opinion in Cell Biology, vol. 16, no. 1, pp. 94–98, 2004.
- K. Burridge and L. Connell, “Talin: a cytoskeletal component concentrated in adhesion plaques and other sites of actin-membrane interaction,” Cell Motility, vol. 3, no. 5-6, pp. 405–417, 1983.
- D. R. Critchley, “Smurf1 zaps the talin head,” Nature Cell Biology, vol. 11, no. 5, pp. 538–540, 2009.
- I. Fillingham, A. R. Gingras, E. Papagrigoriou et al., “A vinculin binding domain from the talin rod unfolds to form a complex with the vinculin head,” Structure, vol. 13, no. 1, pp. 65–74, 2005.
- P. R. Elliott, B. T. Goult, P. M. Kopp et al., “The structure of the talin head reveals a novel extended conformation of the FERM domain,” Structure, vol. 18, no. 10, pp. 1289–1299, 2010.
- B. T. Goult, M. Bouaouina, P. R. Elliott et al., “Structure of a double ubiquitin-like domain in the talin head: a role in integrin activation,” EMBO Journal, vol. 29, no. 6, pp. 1069–1080, 2010.
- W. H. Ziegler, A. R. Gingras, D. R. Critchley, and J. Emsley, “Integrin connections to the cytoskeleton through talin and vinculin,” Biochemical Society Transactions, vol. 36, no. 2, pp. 235–239, 2008.
- P. R. J. Bois, B. P. O'Hara, D. Nietlispach, J. Kirkpatrick, and T. Izard, “The vinculin binding sites of talin and α-actinin are sufficient to activate vinculin,” Journal of Biological Chemistry, vol. 281, no. 11, pp. 7228–7236, 2006.
- D. M. Cohen, B. Kutscher, H. Chen, D. B. Murphy, and S. W. Craig, “A conformational switch in vinculin drives formation and dynamics of a talin-vinculin complex at focal adhesions,” Journal of Biological Chemistry, vol. 281, no. 23, pp. 16006–16015, 2006.
- D. J. Webb, J. T. Parsons, and A. F. Horwitz, “Adhesion assembly, disassembly and turnover in migrating cells—over and over and over again,” Nature Cell Biology, vol. 4, no. 4, pp. E97–E100, 2002.
- J. A. Broussard, D. J. Webb, and I. Kaverina, “Asymmetric focal adhesion disassembly in motile cells,” Current Opinion in Cell Biology, vol. 20, no. 1, pp. 85–90, 2008.
- M. Vicente-Manzanares, C. K. Choi, and A. R. Horwitz, “Integrins in cell migration—the actin connection,” Journal of Cell Science, vol. 122, no. 2, pp. 199–206, 2009.
- J. V. Small, T. Stradal, E. Vignal, and K. Rottner, “The iamellipodium: where motility begins,” Trends in Cell Biology, vol. 12, no. 3, pp. 112–120, 2002.
- E. J. Ezratty, C. Bertaux, E. E. Marcantonio, and G. G. Gundersen, “Clathrin mediates integrin endocytosis for focal adhesion disassembly in migrating cells,” Journal of Cell Biology, vol. 187, no. 5, pp. 733–747, 2009.
- E. J. Ezratty, M. A. Partridge, and G. G. Gundersen, “Microtubule-induced focal adhesion disassembly is mediated by dynamin and focal adhesion kinase,” Nature Cell Biology, vol. 7, no. 6, pp. 581–590, 2005.
- O. Krylyshkina, I. Kaverina, W. Kranewitter et al., “Modulation of substrate adhesion dynamics via microtubule targeting requires kinesin-1,” Journal of Cell Biology, vol. 156, no. 2, pp. 349–359, 2002.
- I. Kaverina, O. Krylyshkina, and J. V. Small, “Microtubule targeting of substrate contacts promotes their relaxation and dissociation,” Journal of Cell Biology, vol. 146, no. 5, pp. 1033–1043, 1999.
- A. Mai, S. Veltel, T. Pellinen et al., “Competitive binding of Rab21 and p120RasGAP to integrins regulates receptor traffic and migration,” Journal of Cell Biology, vol. 194, no. 2, pp. 291–306, 2011.
- J. C. Simpson, G. Griffiths, M. Wessling-Resnick, J. A. M. Fransen, H. Bennett, and A. T. Jones, “A role for the small GTPase Rab21 in the early endocytic pathway,” Journal of Cell Science, vol. 117, no. 26, pp. 6297–6311, 2004.
- C. Margadant, H. N. Monsuur, J. C. Norman, and A. Sonnenberg, “Mechanisms of integrin activation and trafficking,” Current Opinion in Cell Biology, vol. 23, no. 5, pp. 607–614, 2011.
- D. J. Webb, K. Donais, L. A. Whitmore et al., “FAK-Src signalling through paxillin, ERK and MLCK regulates adhesion disassembly,” Nature Cell Biology, vol. 6, no. 2, pp. 154–161, 2004.
- S. J. Franco, M. A. Rodgers, B. J. Perrin et al., “Calpain-mediated proteolysis of talin regulates adhesion dynamics,” Nature Cell Biology, vol. 6, no. 10, pp. 977–983, 2004.
- M. Nagano, D. Hoshino, T. Sakamoto, N. Kawasaki, N. Koshikawa, and M. Seiki, “ZF21 protein regulates cell adhesion and motility,” Journal of Biological Chemistry, vol. 285, no. 27, pp. 21013–21022, 2010.
- T. Watanabe, J. Noritake, and K. Kaibuchi, “Regulation of microtubules in cell migration,” Trends in Cell Biology, vol. 15, no. 2, pp. 76–83, 2005.
- N. Hirokawa, Y. Noda, Y. Tanaka, and S. Niwa, “Kinesin superfamily motor proteins and intracellular transport,” Nature Reviews Molecular Cell Biology, vol. 10, no. 10, pp. 682–696, 2009.
- K. Kawaguchi, “Energetics of kinesin-1 stepping mechanism,” FEBS Letters, vol. 582, no. 27, pp. 3719–3722, 2008.
- D. Ilic, Y. Furuta, S. Kanazawa et al., “Reduced cell motility and enhanced focal adhesion contact formation in cells from FAK-deficient mice,” Nature, vol. 377, no. 6549, pp. 539–544, 1995.
- A. H. Chishti, A. C. Kim, S. M. Marfatia et al., “The FERM domain: a unique module involved in the linkage of cytoplasmic proteins to the membrane,” Trends in Biochemical Sciences, vol. 23, no. 8, pp. 281–282, 1998.
- M. C. Frame, H. Patel, B. Serrels, D. Lietha, and M. J. Eck, “The FERM domain: organizing the structure and function of FAK,” Nature Reviews Molecular Cell Biology, vol. 11, no. 11, pp. 802–814, 2010.
- D. J. Sieg, C. R. Hauck, D. Ilic et al., “FAK integrates growth-factor and integrin signals to promote cell migration,” Nature Cell Biology, vol. 2, no. 5, pp. 249–256, 2000.
- M. D. Schaller, C. A. Otey, J. D. Hildebrand, and J. T. Parsons, “Focal adhesion kinase and paxillin bind to peptides mimicking β integrin cytoplasmic domains,” Journal of Cell Biology, vol. 130, no. 5, pp. 1181–1187, 1995.
- D. Lietha, X. Cai, D. F. J. Ceccarelli, Y. Li, M. D. Schaller, and M. J. Eck, “Structural basis for the autoinhibition of focal adhesion kinase,” Cell, vol. 129, no. 6, pp. 1177–1187, 2007.
- C. Recher, L. Ysebaert, O. Beyne-Rauzy et al., “Expression of focal adhesion kinase in acute myeloid leukemia is associated with enhanced blast migration, increased cellularity, and poor prognosis,” Cancer Research, vol. 64, no. 9, pp. 3191–3197, 2004.
- G. W. McLean, N. O. Carragher, E. Avizienyte, J. Evans, V. G. Brunton, and M. C. Frame, “The role of focal-adhesion kinase in cancer—a new therapeutic opportunity,” Nature Reviews Cancer, vol. 5, no. 7, pp. 505–515, 2005.
- S. G. Clark, D. L. Shurland, E. M. Meyerowitz, C. I. Bargmann, and A. M. Van Der Bliek, “A dynamin GTPase mutation causes a rapid and reversible temperature-inducible locomotion defect in C. elegans,” Proceedings of the National Academy of Sciences of the United States of America, vol. 94, no. 19, pp. 10438–10443, 1997.
- H. S. Shpetner and R. B. Vallee, “Dynamin is a GTPase stimulated to high levels of activity by microtubules,” Nature, vol. 355, no. 6362, pp. 733–735, 1992.
- R. Espejo, W. Rengifo-Cam, M. D. Schaller, B. M. Evers, and S. K. Sastry, “PTP-PEST controls motility, adherens junction assembly, and Rho GTPase activity in colon cancer cells,” American Journal of Physiology—Cell Physiology, vol. 299, no. 2, pp. C454–C463, 2010.
- Y. Zheng, Y. Xia, D. Hawke et al., “FAK phosphorylation by ERK primes ras-induced tyrosine dephosphorylation of FAK mediated by PIN1 and PTP-PEST,” Molecular Cell, vol. 35, no. 1, pp. 11–25, 2009.
- Y. Zheng, W. Yang, Y. Xia, D. Hawke, D. X. Liu, and Z. Lu, “Ras-induced and ERK1/2 phosphorylation-dependent isomerization of PTP-PEST by PIN1 promotes FAK dephosphorylation by PTP-PEST,” Molecular and Cellular Biology, vol. 31, no. 21, pp. 4258–4269, 2011.
- R. Tsutsumi, A. Takahashi, T. Azuma, H. Higashi, and M. Hatakeyama, “Focal adhesion kinase is a substrate and downstream effector of SHP-2 complexed with Helicobacter pylori CagA,” Molecular and Cellular Biology, vol. 26, no. 1, pp. 261–276, 2006.
- M. V. de Oliveira, T. M. Marin, C. F. Clemente, A. P. D. Costa, C. C. Judice, and K. G. Franchini, “SHP-2 regulates myogenesis by coupling to FAK signaling pathway,” FEBS Letters, vol. 583, no. 18, pp. 2975–2981, 2009.
- T. Matozaki, Y. Murata, Y. Saito, H. Okazawa, and H. Ohnishi, “Protein tyrosine phosphatase SHP-2: a proto-oncogene product that promotes Ras activation,” Cancer Science, vol. 100, no. 10, pp. 1786–1793, 2009.
- D. H. Yu, C. K. Qu, O. Henegariu, X. Lu, and G. S. Feng, “Protein-tyrosine phosphatase Shp-2 regulates cell spreading, migration, and focal adhesion,” Journal of Biological Chemistry, vol. 273, no. 33, pp. 21125–21131, 1998.
- F. Liang, S. Y. Lee, J. Liang, D. S. Lawrence, and Z. Y. Zhang, “The role of protein-tyrosine phosphatase 1B in integrin signaling,” Journal of Biological Chemistry, vol. 280, no. 26, pp. 24857–24863, 2005.
- Z. Zhang, S. Y. Lin, B. G. Neel, and B. Haimovich, “Phosphorylated α-actinin and protein-tyrosine phosphatase 1B coregulate the disassembly of the focal adhesion kinase·Src complex and promote cell migration,” Journal of Biological Chemistry, vol. 281, no. 3, pp. 1746–1754, 2006.
- A. Glading, D. A. Lauffenburger, and A. Wells, “Cutting to the chase: calpain proteases in cell motility,” Trends in Cell Biology, vol. 12, no. 1, pp. 46–54, 2002.
- S. J. Franco and A. Huttenlocher, “Regulating cell migration: calpains make the cut,” Journal of Cell Science, vol. 118, no. 17, pp. 3829–3838, 2005.
- K. T. Chan, D. A. Bennin, and A. Huttenlocher, “Regulation of adhesion dynamics by calpain-mediated proteolysis of focal adhesion kinase (FAK),” Journal of Biological Chemistry, vol. 285, no. 15, pp. 11418–11426, 2010.
- C. L. Cortesio, L. R. Boateng, T. M. Piazza, D. A. Bennin, and A. Huttenlocher, “Calpain-mediated proteolysis of paxillin negatively regulates focal adhesion dynamics and cell migration,” Journal of Biological Chemistry, vol. 286, no. 12, pp. 9998–10006, 2011.
- M. Frame and J. Norman, “A tal(in) of cell spreading,” Nature Cell Biology, vol. 10, no. 9, pp. 1017–1019, 2008.
- A. Bhatt, I. Kaverina, C. Otey, and A. Huttenlocher, “Regulation of focal complex composition and disassembly by the calcium-dependent protease calpain,” Journal of Cell Science, vol. 115, no. 17, pp. 3415–3425, 2002.
- M. Nagano, D. Hoshino, T. Sakamoto, T. Akizawa, N. Koshikawa, and M. Seiki, “ZF21 is a new regulator of focal adhesion disassembly and a potential member of the spreading initiation center,” Cell Adhesion and Migration, vol. 5, no. 1, pp. 23–28, 2011.
- N. O. Carragher, M. A. Westhoff, V. J. Fincham, M. D. Schaller, and M. C. Frame, “A novel role for FAK as a protease-targeting adaptor protein: regulation by p42 ERK and Src,” Current Biology, vol. 13, no. 16, pp. 1442–1450, 2003.
- H. Stenmark and R. Aasland, “FYVE-finger proteins—effectors of an inositol lipid,” Journal of Cell Science, vol. 112, part 23, pp. 4175–4183, 1999.
- M. Nagano, D. Hoshino, S. Koshiba et al., “ZF21 protein, a regulator of the disassembly of focal adhesions and cancer metastasis, contains a novel noncanonical pleckstrin homology domain,” Journal of Biological Chemistry, vol. 286, no. 36, pp. 31598–31609, 2011.
- E. Cukierman, R. Pankov, D. R. Stevens, and K. M. Yamada, “Taking cell-matrix adhesions to the third dimension,” Science, vol. 294, no. 5547, pp. 1708–1712, 2001.
- S. I. Fraley, Y. Feng, R. Krishnamurthy et al., “A distinctive role for focal adhesion proteins in three-dimensional cell motility,” Nature Cell Biology, vol. 12, no. 6, pp. 598–604, 2010.
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