Review Article | Open Access
Beina Teng, Alexander Lukasz, Mario Schiffer, "The ADF/Cofilin-Pathway and Actin Dynamics in Podocyte Injury", International Journal of Cell Biology, vol. 2012, Article ID 320531, 8 pages, 2012. https://doi.org/10.1155/2012/320531
The ADF/Cofilin-Pathway and Actin Dynamics in Podocyte Injury
ADF/cofilins are the major regulators of actin dynamics in mammalian cells. The activation of ADF/cofilins is controlled by a variety of regulatory mechanisms. Dysregulation of ADF/cofilin may result in loss of a precisely organized actin cytoskeletal architecture and can reduce podocyte migration and motility. Recent studies suggest that cofilin-1 can be regulated through several extracellular signals and slit diaphragm proteins. Cofilin knockdown and knockout animal models show dysfunction of glomerular barrier and filtration with foot process effacement and loss of secondary foot processes. This indicates that cofilin-1 is necessary for modulating actin dynamics in podocytes. Podocyte alterations in actin architecture may initiate or aid the progression of a large variety of glomerular diseases, and cofilin activity is required for reorganization of an intact filtration barrier. Since almost all proteinuric diseases result from a similar phenotype with effacement of the foot processes, we propose that cofilin-1 is at the centre stage of the development of proteinuria and thus may be an attractive drug target for antiproteinuric treatment strategies.
Glomerular visceral epithelial cells (podocytes) play a central role in maintenance of the Glomerular Filtration barrier by preventing the loss of high-molecular-weight molecules. The podocyte is a highly specialized and polarized cell type that consists of three parts: the cell body, the primary foot processes, and the secondary foot processes. The interdigitating foot processes completely cover the outer surface of the glomerular capillary and form a filtration slit that is spanned by a membranelike structure; this is called the slit diaphragm . Actin filaments are the structural backbone component of podocyte foot processes. Protein complexes of slit diaphragm that regulate or stabilize the actin cytoskeleton are therefore essential for the maintenance of an intact glomerular filtration barrier . When podocytes are injured, they undergo dramatic actin cytoskeletal changes. These cytoskeletal changes lead to retraction of secondary processes and loss of functional filtration slits; this is termed foot process effacement. Foot process effacement is a dynamic and reversible process that contributes to the development of massive proteinuria in human glomerular diseases .
Actin is one of the most abundant and highly conserved proteins in many eukaryotic cells. It is involved in many different cellular processes that are essential for cell growth, differentiation, division, membrane organization, and motility . The dynamics of actin filaments (F-actin) assembly/disassembly and organization in cells are regulated by several actin-binding proteins, including the Arp2/3 complex, profilin, capping protein, and ADF/cofilins.
One of the dynamic processes in the cell that is controlled by F-actin assembly and disassembly is the lamellipodium. The lamellipodium of motile cell is predominantly composed of actin filaments, meaning that regulation of actin filament arrangement at the leading edge is necessary for the cellular directional motility .
ADF/cofilins are ubiquitous among eukaryotes and are essential proteins responsible for the turnover and reorganization of actin filaments in vivo [6, 7]. Mammals express three members of the ADF/cofilin (AC) family: actin depolymerising factor (ADF), nonmuscle cofilin (cofilin-1, Cfl1), and muscle cofilin (cofilin-2, Cfl2). Cofilin-1 is expressed in most cell types during development . Cofilin-2 has two splice variants (Cfl2a and Cfl2b); Cfl2b is expressed; predominantly in muscle cells, while Cfl2a in several tissues . Expression of ADF is restricted to endothelia and epithelia . These three isoforms share similar but not identical biochemical activities. Only cofilin-1 and ADF are expressed in cultured human and mouse podocytes, with cofilin-1 being the predominant isoform [10, 11].
In this paper, we focus on the regulatory mechanisms of ADF/cofilin proteins in the modulation of actin dynamics in podocytes. We discuss how alterations in these processes can lead to a common phenotype in large variety of human glomerular diseases due to rearrangement of actin filaments in podocyte foot processes.
2. Modulation of Actin Dynamics by ADF/Cofilin
Actin filaments are a highly dynamic part of the cell which undergoes constant assembly and disassembly. Actin filaments are polymers that are composed of globular actin subunits. Each subunit is structurally polar and arranged head to tail to give the filament an overall structural polarity . ADF/cofilin proteins modulate the actin dynamics during the mechanism of treadmilling.
Treadmilling is the dynamic process in which the overall length of the filaments remains approximately constant, but moves through growing at one end (plus, fast growing, or barbed end) by association of ATP-actin subunits (ATP-G-actin) and shrinking at the other end (minus, slow growing, or pointed end) through disassociation of monomers by ATP hydrolysis. When incorporated ATP-actin monomers undergo hydrolysis of ATP to form ADP-actin; cofilin, which displays greater affinity for ADP-actin, binds to ADP-actin which dissociates from the actin filament and recycles back to the monomer pool. ADF/cofilin proteins have the ability to enhance the rate of ADP-actin disassembly from the pointed end, which can be observed in the leading edge of motile cells . Moreover, binding of ADF/cofilins to ADP actin filament destabilizes a twisted form of the actin filament [14, 15] and promotes severing of the filaments into short segments which increases the number of depolymerizing ends . ADF/cofilin molecules binding to ADP-actin monomer can also inhibit nucleotide exchange to prevent its entrance to a new polymerization cycle .
On the other hand, ADF/cofilin proteins can accelerate spontaneous polymerization of monomers (nucleation) to initiate a new filament . Nucleation of new filaments is dependent on ADF/cofilins concentration. At a low concentration, ADF/cofilin proteins have the highest-F-actin-severing activity but F-actin is stabilized and aged by ADF/cofilin decoration at higher concentration, and at a very high concentration, cofilin is able to nucleate new filaments . This makes ADF/cofilin an important regulator not only of actin depolymerisation but also of actin stability and nucleation.
All three mammalian ADF/cofilin isoforms have a nuclear translocation sequence, perhaps enabling a ADF/cofilin-actin complex to pass into the nucleus . The actin sequence lacks nuclear translocation signal but does have an export sequence . With a molecular weight of 42 kDa, it is unlikely for actin to enter the nucleus by diffusion; therefore it relies on ADF/cofilin as transporter proteins to mediate its entry into the nucleus .
3. Regulation of ADF/Cofilins
In mammals phosphorylation of ADF/cofilin on Ser3 leads to inactivation [22, 23] but does not alter the protein conformation, while phosphorylation prevents G- and F-actin binding and tends to stabilize F-actin by inhibiting the ability of these protein to sever and depolymerize F-actin .
Phosphorylation of ADF/cofilins is mainly regulated by two kinase families, the LIM kinases (LIMK1, 2) [25, 26] and testicular protein kinases (TESK1, 2) [27, 28]. TESK expression is restricted to several tissues such as testis, brain, kidney, heart, and lung . The most well-known pathways involved in the TESK activation are very different and mainly mediated by integrins and adhesion dependent [27, 28, 30]. LIM kinases are ubiquitously expressed and are downstream targets of small Rho-GTPases. Both LIMK1 and LIMK2, are targets of Rho-GTPases (Rho and Cdc42) via Rho kinases (ROCK1, ROCK2) and myotonic dystrophy kinase-related Cdc42-binding protein kinase (MRCKα), respectively [31, 32]. LIMK1, but not LIMK2 can be activated by p21-activated kinases (PAK1, PAK2, and PAK4), downstream of Rac and Cdc42 activation [33, 34]. In addition, LIMK1 can be activated in a Rho GTPase-independent manner [35, 36]. These evidences suggest that small Rho GTPases might regulate various actin-dependent cell functions through ADF/cofilin activity to maintain the structure and physiological function of adult kidneys.
ADF/cofilin can be dephosphorylated, and therefore activated, by two phosphatases, the slingshot family (SSH1L, SSH2L, and SSH3L) and chronophin (CIN) [37, 38]. CIN is highly specific for cofilin but the upstream signalling pathways remain a mystery . SSH is the only known phosphatase to dephosphorylate and inactivate both LIMK1 and LIMK2 which leads to activation of ADF/cofilin by negative regulation of a negative regulator. Only a few regulatory pathways resulting in SSH activation have been ever identified. The phosphatase activity of SSH1L is negatively regulated via phosphorylation by PAK4 in different cell types . It suggests a negative regulation of Rac1 activation on SSH and ADF/cofilin activity. In other cell types, SSH is activated through integrin pathway via Rac1 activation . A colocalization of SSH and actin filaments together with the locatized activation of SSH1L was observed in vitro, indicating that assembly of F-actin can trigger the local activation of SSH1L and therefore promotes cofilin-mediated actin turnover in protrusive lamellipodia . Some scaffolding proteins such as 14-3-3 can also participate in the modulation of ADF/cofilin-activity through interaction with SSH isoforms. Phosphorylation of SSH1L on serines 937 and 978 by protein kinase D (PKD) promotes the interaction of 14-3-3 with SSH1L and restricts its subcellular localization, which may inhibit SSH activity in breast carcinoma cells . Furthermore, the activity of nonphosphorylated cofilin can be inhibited by binding to phosphatidylinositol 4,5-bisphosphate (PIP2), which prevents cofilin interaction with actin, but phospholipase-C- (PLC-) mediated PIP2 reduction causes cofilin to be released to cell membrane and to be activated . As mentioned above, extracellular signals can regulate actin dynamics through ADF/cofilin and its upstream regulators (Figure 1).
ADF/cofilin can also be mechanically controlled by intracellular pH both in vivo and in vitro [44, 45]. Changes in pH over the physiological range alter the severing capacity of active ADF/cofilin in vitro, but interestingly ADF is more sensitive to pH variation than cofilin . Overall, the regulation of ADF/cofilin can be influenced by subcellular localization of ADF/cofilin kinases and phosphatases and synergistic-or competitive interactions of ADF/cofilins with other actin-binding proteins (ABPs) .
4. Regulation of ADF/Cofilin Activity in Podocytes
Experimental evidence indicates that nephrin, an Ig-G-like protein which is specifically expressed in podocytes is also engaged in regulating cofilin-1 and actin reorganization . Garg et al. demonstrated that cofilin-1 colocalized at the plasma membrane with nephrin in vitro. Nephrin-induced activation of phosphatidylinositol 3 kinase (PI3K) is necessary for SSH1L dephosphorylation via an unknown phosphatase. SSH1L activation leads to cofilin-1 activation through LIMK dephosphorylation on Thr508 . On the other hand, dephosphorylation of SSH1L decreases the affinity for 14-3-3, and the released SSH1L translocates to the protrusive leading edge of podocyte to activate cofilin-1- mediated actin remodelling. It still has to be clarified whether PKD is also involved in this regulation pathway in podocytes. Thus far no evidence was published that demonstrates that PKD can regulate SSH1L activity in podocytes.
Cofilin-1 activity can also be altered in response to several extracellular stimuli. Incubation of murine and human podocytes with TGF-β, a podocyte stressor, leads to increased cofilin-1 phosphorylation and decreased cofilin-1 activation . In contrast, when stimulated with phorbol 12-myristate 13-acetate (PMA), increased activation of cofilin-1 was observed in murine and human podocytes. PMA activates PKC, a well-known regulator of actin cytoskeleton dynamics in large variety of cells . Taken together, this suggests that PKC may be also involved in the pathway of modulating cofilin-1 activity. In human neutrophils, a PKC-dependent phosphorylation of cofilin was observed, but the involved PKC isoforms and the regulatory pathway remain to be demonstrated .
Experiments in vitro have proved that mechanical stress can change the podocyte morphology and the actin organization . Osmotic stress, a major mechanical stress, has also been addressed to the cofilin-related regulation. In kidney tubular cells, hyperosmotic stress induces cofilin phosphorylation via Rho/ROCK/LIMK pathway and slightly delays actin kinetics due to reduced cofilin activation . This same pathway was also activated by high-glucose treatment in cultured proximal tubular epithelial cells (PTECs), resulting in time-dependent increases in p-cofilin and pLIMK. Moreover, high glucose induced membrane translocation of Rho and ROCK2, without altering the PI3K-pathway, SSH1L, Rac/PAK, LIMK expression, or cofilin and SSH1L regulation at both mRNA and protein levels . These studies highlight the possibility that osmotic stress or high glucose level may play a regulatory role in podocyte actin cytoskeleton through altering cofilin phosphorylation.
The motility and migration of podocytes can therefore be dramatically altered, when the expression level or activities of kinases or phosphatases that regulate ADF/cofilin is varied.
5. Podocyte Injury Associated with ADF/Cofilin Inactivation
The podocyte foot process contains a coordinated network of actin filaments which are connected by a multiprotein complex to the slit diaphragm and the glomerular basement membrane (GBM) via adhesion proteins. Proteins regulating or stabilizing the actin cytoskeleton are therefore essential for the maintenance of glomerular filtration function [51–53]. Rearrangement of the actin cytoskeleton and dysregulation of its associated proteins is the major cause of foot process effacement and proteinuria . Foot process effacement can be observed in a variety of human and experimental glomerular diseases associated with massive proteinuria, including minimal change disease, focal segmental glomerulosclerosis (FSGS), membranous glomerulopathy, IgA-nephropathy, diabetic nephropathy, and lupus nephritis [55, 56]. Mutation of actin-binding proteins including α-actinin-4, MYH9, INF2, and CD2AP in podocytes leads to rearrangement of actin cytoskeleton, disruption of filtration barrier, and subsequent kidney failure [57–61].
There is ever-increasing evidence indicating that ADF/cofilins are the major regulators participating in actin turnover and cytoskeletal reorganization to sustain an intact podocyte foot processes. Different animal vertebrate models like knockdown or mutation of cofilin-1 in zebrafish or podocyte-specific knockout in mice have been performed to confirm the affect of cofilin-1 deficiency in vivo. Deficiency of cofilin-1 in zebrafish leads to a severe edematous phenotype, effacement of podocyte foot processes, and dysfunction of the glomerular filtration barrier indicating that cofilin-1 is an indispensable factor for the integrity of normal podocyte foot processes in zebrafish .
Mutant mice with podocyte-specific cofilin-1 deletion show disruption of renal function and alteration in podocytes foot processes at 6 months of age. The mutant mice have severe proteinuria and indiscernible foot process spreading. However podocyte foot processes remain intact in newborn mutant mice . The delayed set-on of proteinuria and podocyte foot process effacement can be explained by compensation of increased ADF isoform expression during the early development of mutant mice. Some studies revealed that the different isoforms of ADF/cofilin are not completely redundant. ADF is efficient at turning over actinafilaments, whereas cofilin-1 is a more effective nucleator of new filament assembly [8, 62, 63]. However, due to the differences in actin modulation functions, ADF can not completely compensate the lost function of cofilin-1 and it does not stay continuously upregulated in the podocytes-specific knockout mouse. Interestingly, proteinuria and phenotypic changes coincide with downregulated ADF expression .
In vitro cofilin-1 deficiency does not lead to significant changes in actin architecture in podocytes. Supression of cofilin-1 expression in cultured podocytes resulted in a limited breakdown and formation of new actin filaments beneath the plasma membrane and loss of forward pressure on the overlying membrane, which leads to a reduced cellular migration activity, suggesting that cofilin-1 activity is required for rapid actin turnover in the lamellipodial protrusion and is necessary for directional cellular migration activity in podocytes [10, 11]. However, regulation of actin dynamics is not the only role of actin to maintain the podocyte function. Obrdlik and Percipalle. showed that cofilin-1 is required for elongation of RNA polymerase-II-mediated transcription through interaction with actin . This study indirectly indicates that deletion or downregulation of cofilin-1 might disrupt the transcription of nascent genes that are essential for podocyte integrity.
Despite wide distribution of cofilin modulators genes, deletion of these genes resulted in relatively mild phenotypes in mice. Deficiency of LIMK-1 led to abnormalities in synaptic structure and spine development, due to aberrant regulation of the actin cytoskeleton in vivo . LIMK-2 knockout mice exhibited minimal abnormalities, while the double LIMK-1/LIMK-2 null mice were more severely impaired but not embryonic lethal . These morphological and functional changes were primarily observed in the neuronal system, but still suggest the possibility that LIMK deficiency might cause similar abnormalities in podocyte structure and function. SSH3L knockout mice were made to examine its potential roles in vivo. Unexpectedly SSH3L was not essential for viability or development of epithelial tissues . An SSH1L or SSH2L deficiency in animal models or human diseases was not yet reported.
Under pathological conditions in the kidney, alterations of the extracellular milieu also change cofilin-1 activity. TGF-β is described as a causative factor for initiation and progression of proteinuric diseases in mice and humans. TGF-β accumulates in injured kidneys in experimental animal models and chronic renal disease in humans [68, 69]. In different disease states TGF-β activation induces a constant cofilin-1 inactivation, which results in disruption of cofilin-1-mediated actin dynamins and subsequently effacement of podocytes and proteinuria. TGF-β is an important mediator of progressive fibrosis, cell proliferation, and cell death in glomerular diseases. TGF-β pathways also occupy a central position in signalling networks that control a diverse set of cellular processes. Interestingly, the effects of TGF-β on podocytes are concentration dependent . Our group is currently investigating whether TGF-β impairs directional migration activity and leads to alternations in cytoskeleton arrangement in a concentration-dependent manner in podocytes. As mentioned above, PMA and TGF-β have opposite effect on cofilin phosphorylation. PMA has already been shown to increase migration in several cell types [71, 72]. However, it is still unknown whether PMA can rescue the dysfunction of TGF-β induced in podocytes.
Hyperglycemia is a prerequisite for development of diabetic nephropathty. Hyperglycemia induces increased osmolarity of blood serum. In diabetic mellitus the high glucose level and hyperosmolarity could promote the Rho/ROCK activation in podocytes, because abundant evidence identified high glucose and osmotic stress as stimulators of Rho-ROCK signalling pathway [73–76]. It suggests that Rho activation can cause cofilin phosphorylation and inactivation in podocytes. The disruption of actin dynamic via cofilin inactivation dependent on the hyperglycemia and hyperosmotic stress is one of the causative stimulators for the progressive development of diabetic nephropathy. In addition, high glucose level leads to increased expression of TGF-β , which further enhances the cofilin inactivation and its nuclear localization. In response to the stimulation by TGF-β, phosphorylated cofilin-1 undergoes nuclear translocation in both murine and human podocytes (our unpublished data).
In renal diseases associated with foot process effacement, cofilin-1 was inactivated and translocated to the nucleus of podocytes. Cofilin-1 is dephosphorylated and active under normal homeostasis conditions. In contrast, when the podocytes undergo foot process effacement because of nephritic glomerular diseases, cofilin-1 was found phosphorylated (inactivated) and translocated to the nucleus of podocytes  (Figure 2). This suggested that cofilin-1 can be a potential diagnostic marker to detect the injury of podocytes in the glomerulus. The role of nuclear uptake of phosphorylated cofilin is currently unknown. But some evidence indicates that cofilin forms a complex with actin and DNaseI and perhaps plays a role in DNA degradation and initiation of apoptosis . One of the new most interesting findings is that even though cofilin-1 is constantly expressed throughout podocytes, the phosphorylated form is not detectable in the normal glomerulus in mice and humans indicating that all of the cofilins are active. Only if proteinuria is present, there is a dramatic increase in phosphorylated cofilin-1 and nuclear translocation of phosphocofilin is detectable . The expression of phosphorylated cofilin-1 in glomerular diseases suggests a reduced capacity of podocytes to adapt to glomerular pressure differences. A higher filtration pressure and distension of the capillary wall can not be compensated and leads to proteinuria.
Because the glomerular capillary pressure constantly changes with blood pressure, it is likely that the foot processes experience distension of the capillary wall. Therefore, podocytes must be able to adapt to these changes to assure a network of functional filtration slits. Cofilin-1 is therefore necessary for foot process spreading by accelerating actin turnover and gives the pushing force for the protrusive leading edge. When podocytes are injured, cofilin-1 is required to restore the normal actin architecture of podocytes for recovery. Otherwise, this injury is not reversible and results in renal diseases associated with podocytes effacement and massive proteinuria. Thus, cofilin dephosphorylation might be an attractive pharmacological target to ensure proper actin turnover in proteinuric diseases which might help in the recovery process of effacement.
The authors thank Nathan Susnik for critically reading and improving this paper.
- H. Pavenstädt, W. Kriz, and M. Kretzler, “Cell biology of the glomerular podocyte,” Physiological Reviews, vol. 83, no. 1, pp. 253–307, 2003.
- K. Tryggvason, J. Patrakka, and J. Wartiovaara, “Hereditary proteinuria syndromes and mechanisms of proteinuria,” The New England Journal of Medicine, vol. 354, no. 13, pp. 1387–1401, 2006.
- W. Kriz and K. V. Lemley, “The role of the podocyte in glomerulosclerosis,” Current Opinion in Nephrology and Hypertension, vol. 8, no. 4, pp. 489–497, 1999.
- J. R. Bamburg and O. P. Wiggan, “ADF/cofilin and actin dynamics in disease,” Trends in Cell Biology, vol. 12, no. 12, pp. 598–605, 2002.
- T. D. Pollard and G. G. Borisy, “Cellular motility driven by assembly and disassembly of actin filaments,” Cell, vol. 112, no. 4, pp. 453–465, 2003.
- J. R. Bamburg, “Proteins of the ADF/cofilin family: essential regulators of actin dynamics,” Annual Review of Cell and Developmental Biology, vol. 15, pp. 185–230, 1999.
- T. M. Svitkina and G. G. Borisy, “Arp2/3 complex and actin depolymerizing factor/cofilin in dendritic organization and treadmilling of actin filament array in lamellipodia,” Journal of Cell Biology, vol. 145, no. 5, pp. 1009–1026, 1999.
- M. K. Vartiainen, T. Mustonen, P. K. Mattila et al., “The three mouse actin-depolymerizing factor/cofilins evolved to fulfill cell-type-specific requirements for actin dynamics,” Molecular Biology of the Cell, vol. 13, no. 1, pp. 183–194, 2002.
- C. Thirion, R. Stucka, B. Mendel et al., “Characterization of human muscle type cofilin (CFL2) in normal and regenerating muscle,” European Journal of Biochemistry, vol. 268, no. 12, pp. 3473–3482, 2001.
- S. Ashworth, B. Teng, J. Kaufeld et al., “Cofilin-1 inactivation leads to proteinuria—studies in zebrafish, mice and humans,” PLoS ONE, vol. 5, no. 9, Article ID e12626, pp. 1–10, 2010.
- P. Garg, R. Verma, L. Cook et al., “Actin-depolymerizing factor cofilin-1 is necessary in maintaining mature podocyte architecture,” Journal of Biological Chemistry, vol. 285, no. 29, pp. 22676–22688, 2010.
- J. V. Small, G. Isenberg, and J. E. Celis, “Polarity of actin at the leading edge of cultured cells,” Nature, vol. 272, no. 5654, pp. 638–639, 1978.
- M. F. Carlier, V. Laurent, J. Santolini et al., “Actin depolymerizing factor (ADF/cofilin) enhances the rate of filament turnover: implication in actin-based motility,” Journal of Cell Biology, vol. 136, no. 6, pp. 1307–1322, 1997.
- A. McGough, B. Pope, W. Chiu, and A. Weeds, “Cofilin changes the twist of F-actin: implications for actin filament dynamics and cellular function,” Journal of Cell Biology, vol. 138, no. 4, pp. 771–781, 1997.
- V. E. Galkin, A. Orlova, N. Lukoyanova, W. Wriggersd, and E. H. Egelman, “Actin depolymerizing factor stabilizes an existing state of F-actin and can change the tilt of F-actin subunits,” Journal of Cell Biology, vol. 153, no. 1, pp. 75–86, 2001.
- I. Ichetovkin, J. Han, K. M. Pang, D. A. Knecht, and J. S. Condeelis, “Actin filaments are severed by both native and recombinant Dictyostelium cofilin but to different extents,” Cell Motility and the Cytoskeleton, vol. 45, no. 4, pp. 293–306, 2000.
- E. Nishida, “Opposite effects of cofilin and profilin from porcine brain on rate of exchange of actin-bound adenosine 5′-triphosphate,” Biochemistry, vol. 24, no. 5, pp. 1160–1164, 1985.
- D. S. Kudryashov, V. E. Galkin, A. Orlova, M. Phan, E. H. Egelman, and E. Reisler, “Cofilin cross-bridges adjacent actin protomers and replaces part of the longitudinal F-actin interface,” Journal of Molecular Biology, vol. 358, no. 3, pp. 785–797, 2006.
- E. Andrianantoandro and T. D. Pollard, “Mechanism of actin filament turnover by severing and nucleation at different concentrations of ADF/cofilin,” Molecular Cell, vol. 24, no. 1, pp. 13–23, 2006.
- E. Nishida, K. Iida, N. Yonezawa, S. Koyasu, I. Yahara, and H. Sakai, “Cofilin is a component of intranuclear and cytoplasmic actin rods induced in cultured cells,” Proceedings of the National Academy of Sciences of the United States of America, vol. 84, no. 15, pp. 5262–5266, 1987.
- A. Wada, M. Fukuda, M. Mishima, and E. Nishida, “Nuclear export of actin: a novel mechanism regulating the subcellular localization of a major cytoskeletal protein,” EMBO Journal, vol. 17, no. 6, pp. 1635–1641, 1998.
- B. J. Agnew, L. S. Minamide, and J. R. Bamburg, “Reactivation of phosphorylated actin depolymerizing factor and identification of the regulatory site,” Journal of Biological Chemistry, vol. 270, no. 29, pp. 17582–17587, 1995.
- K. Moriyama, K. Iida, and I. Yahara, “Phosphorylation of Ser-3 of cofilin regulates its essential function on actin,” Genes to Cells, vol. 1, no. 1, pp. 73–86, 1996.
- L. Blanchoin, R. C. Robinson, S. Choe, and T. D. Pollard, “Phosphorylation of Acanthamoeba actophorin (ADF/cofilin) blocks interaction with actin without a change in atomic structure,” Journal of Molecular Biology, vol. 295, no. 2, pp. 203–211, 2000.
- S. Arber, F. A. Barbayannis, H. Hanser et al., “Regulation of actin dynamics through phosphorylation of cofilin by LIM- kinase,” Nature, vol. 393, no. 6687, pp. 805–809, 1998.
- N. Yang, O. Higuchi, K. Ohashi et al., “Cofflin phosphorylation by LIM-kinase 1 and its role in Rac-mediated actin reorganization,” Nature, vol. 393, no. 6687, pp. 809–812, 1998.
- J. Toshima, J. Y. Toshima, T. Amano, N. Yang, S. Narumiya, and K. Mizuno, “Cofilin phosphorylation by protein kinase testicular protein kinase 1 and its role in integrin-mediated actin reorganization and focal adhesion formation,” Molecular Biology of the Cell, vol. 12, no. 4, pp. 1131–1145, 2001.
- J. Toshima, J. Y. Toshima, K. Takeuchi, R. Mori, and K. Mizuno, “Cofilin phosphorylation and actin reorganization activities of testicular protein kinase 2 and its predominant expression in testicular Sertoli cells,” Journal of Biological Chemistry, vol. 276, no. 33, pp. 31449–31458, 2001.
- J. Toshima, J. Y. Toshima, M. Suzuki, T. Noda, and K. Mizuno, “Cell-type-specific expression of a TESK1 promoter-linked lacZ gene in transgenic mice,” Biochemical and Biophysical Research Communications, vol. 286, no. 3, pp. 566–573, 2001.
- D. P. LaLonde, M. C. Brown, B. P. Bouverat, and C. E. Turner, “Actopaxin interacts with TESK1 to regulate cell spreading on fibronectin,” Journal of Biological Chemistry, vol. 280, no. 22, pp. 21680–21688, 2005.
- M. Maekawa, T. Ishizaki, S. Boku et al., “Signaling from Rho to the actin cytoskeleton through protein kinases ROCK and LIM-kinase,” Science, vol. 285, no. 5429, pp. 895–898, 1999.
- T. Sumi, K. Matsumoto, Y. Takai, and T. Nakamura, “Cofilin phosphorylation and actin cytoskeletal dynamics regulated by Rho- and Cdc42-activated LIM-kinase 2,” Journal of Cell Biology, vol. 147, no. 7, pp. 1519–1532, 1999.
- C. Dan, A. Kelly, O. Bernard, and A. Minden, “Cytoskeletal changes regulated by the PAK4 serine/threonine kinase are mediated by LIM kinase 1 and cofilin,” Journal of Biological Chemistry, vol. 276, no. 34, pp. 32115–32121, 2001.
- U. K. Misra, R. Deedwania, and S. V. Pizzo, “Binding of activated α2-macroglobulin to its cell surface receptor GRP78 in 1-LN prostate cancer cells regulates PAK-2-dependent activation of LIMK,” Journal of Biological Chemistry, vol. 280, no. 28, pp. 26278–26286, 2005.
- T. M. Leisner, M. Liu, Z. M. Jaffer, J. Chernoff, and L. V. Parise, “Essential role of CIB1 in regulating PAK1 activation and cell migration,” Journal of Cell Biology, vol. 170, no. 3, pp. 465–476, 2005.
- M. Kobayashi, M. Nishita, T. Mishima, K. Ohashi, and K. Mizuno, “MAPKAPK-2-mediated LIM-kinase activation is critical for VEGF-induced actin remodeling and cell migration,” EMBO Journal, vol. 25, no. 4, pp. 713–726, 2006.
- R. Niwa, K. Nagata-Ohashi, M. Takeichi, K. Mizuno, and T. Uemura, “Control of actin reorganization by slingshot, a family of phosphatases that dephosphorylate ADF/cofilin,” Cell, vol. 108, no. 2, pp. 233–246, 2002.
- A. Gohla, J. Birkenfeld, and G. M. Bokoch, “Chronophin, a novel HAD-type serine protein phosphatase, regulates cofilin-dependent actin dynamics,” Nature Cell Biology, vol. 7, no. 1, pp. 21–29, 2005.
- T. Y. Huang, C. Dermardirossian, and G. M. Bokoch, “Cofilin phosphatases and regulation of actin dynamics,” Current Opinion in Cell Biology, vol. 18, no. 1, pp. 26–31, 2006.
- J. Soosairajah, S. Maiti, O. Wiggan et al., “Interplay between components of a novel LIM kinase-slingshot phosphatase complex regulates cofilin,” EMBO Journal, vol. 24, no. 3, pp. 473–486, 2005.
- K. Kligys, J. N. Claiborne, P. J. DeBiase et al., “The slingshot family of phosphatases mediates Rac1 regulation of cofilin phosphorylation, laminin-332 organization, and motility behavior of keratinocytes,” Journal of Biological Chemistry, vol. 282, no. 44, pp. 32520–32528, 2007.
- K. Nagata-Ohashi, Y. Ohta, K. Goto et al., “A pathway of neuregulin-induced activation of cofilin-phosphatase Slingshot and cofilin in lamellipodia,” Journal of Cell Biology, vol. 165, no. 4, pp. 465–471, 2004.
- J. Van Rheenen, X. Song, W. Van Roosmalen et al., “EGF-induced PIP2 hydrolysis releases and activates cofilin locally in carcinoma cells,” Journal of Cell Biology, vol. 179, no. 6, pp. 1247–1259, 2007.
- B. W. Bernstein, W. B. Painter, H. Chen, L. S. Minamide, H. Abe, and J. R. Bamburg, “Intracellular pH modulation of ADF/cofilin proteins,” Cell Motility and the Cytoskeleton, vol. 47, no. 4, pp. 319–336, 2000.
- M. Hawkins, B. Pope, S. K. Maciver, and A. G. Weeds, “Human actin depolymerizing factor mediates a pH-sensitive destruction of actin filaments,” Biochemistry, vol. 32, no. 38, pp. 9985–9993, 1993.
- M. Van Troys, L. Huyck, S. Leyman, S. Dhaese, J. Vandekerkhove, and C. Ampe, “Ins and outs of ADF/cofilin activity and regulation,” European Journal of Cell Biology, vol. 87, no. 8-9, pp. 649–667, 2008.
- S. Djafarzadeh and V. Niggli, “Signaling pathways involved in dephosphorylation and localization of the actin-binding protein cofilin in stimulated human neutrophils,” Experimental Cell Research, vol. 236, no. 2, pp. 427–435, 1997.
- N. Endlich, K. R. Kress, J. Reiser et al., “Podocytes respond to mechanical stress in vitro,” Journal of the American Society of Nephrology, vol. 12, no. 3, pp. 413–422, 2001.
- A. C. P. Thirone, P. Speight, M. Zulys et al., “Hyperosmotic stress induces Rho/Rho kinase/LIM kinase-mediated cofilin phosphorylation in tubular cells: key role in the osmotically triggered F-actin response,” American Journal of Physiology, vol. 296, no. 3, pp. C463–C475, 2009.
- F. Ishibashi, “High glucose increases phosphocofilin via phosphorylation of LIM kinase due to Rho/Rho kinase activation in cultured pig proximal tubular epithelial cells,” Diabetes Research and Clinical Practice, vol. 80, no. 1, pp. 24–33, 2008.
- I. Shirato, T. Sakai, K. Kimura, Y. Tomino, and W. Kriz, “Cytoskeletal changes in podocytes associated with foot process effacement in Masugi nephritis,” American Journal of Pathology, vol. 148, no. 4, pp. 1283–1296, 1996.
- T. Takeda, T. McQuistan, R. A. Orlando, and M. G. Farquhar, “Loss of glomerular foot processes is associated with uncoupling of podocalyxin from the actin cytoskeleton,” Journal of Clinical Investigation, vol. 108, no. 2, pp. 289–301, 2001.
- W. E. Smoyer and P. Mundel, “Regulation of podocyte structure during the development of nephrotic syndrome,” Journal of Molecular Medicine, vol. 76, no. 3-4, pp. 172–183, 1998.
- J. Oh, J. Reiser, and P. Mundel, “Dynamic (re)organization of the podocyte actin cytoskeleton in the nephrotic syndrome,” Pediatric Nephrology, vol. 19, no. 2, pp. 130–137, 2004.
- D. Kerjaschki, “Caught flat-footed: podocyte damage and the molecular bases of focal glomerulosclerosis,” Journal of Clinical Investigation, vol. 108, no. 11, pp. 1583–1587, 2001.
- S. Somlo and P. Mundel, “Getting a foothold in nephrotic syndrome,” Nature Genetics, vol. 24, no. 4, pp. 333–335, 2000.
- J. M. Kaplan, S. H. Kim, K. N. North et al., “Mutations in ACTN4, encoding α-actinin-4, cause familial focal segmental glomerulosclerosis,” Nature Genetics, vol. 24, no. 3, pp. 251–256, 2000.
- C. H. Kos, T. C. Le, S. Sinha et al., “Mice deficient in α-actinin-4 have severe glomerular disease,” Journal of Clinical Investigation, vol. 111, no. 11, pp. 1683–1690, 2003.
- C. Arrondel, N. Vodovar, B. Knebelmann et al., “Expression of the nonmuscle myosin heavy chain IIA in the human kidney and screening for MYH9 mutations in Epstein and Fechtner syndromes,” Journal of the American Society of Nephrology, vol. 13, no. 1, pp. 65–74, 2002.
- E. J. Brown, J. S. Schlöndorff, D. J. Becker et al., “Mutations in the formin gene INF2 cause focal segmental glomerulosclerosis,” Nature Genetics, vol. 42, no. 1, pp. 72–76, 2010.
- J. H. Kim, H. Wu, G. Green et al., “CD2-associated protein haploinsufficiency is linked to glomerular disease susceptibility,” Science, vol. 300, no. 5623, pp. 1298–1300, 2003.
- K. Nakashima, N. Sato, T. Nakagaki, H. Abe, S. Ono, and T. Obinata, “Two mouse cofilin isoforms, muscle-type (MCF) and non-muscle type (NMCF), interact with F-actin with different efficiencies,” Journal of Biochemistry, vol. 138, no. 4, pp. 519–526, 2005.
- S. Yeoh, B. Pope, H. G. Mannherz, and A. Weeds, “Determining the differences in actin binding by human ADF and cofilin,” Journal of Molecular Biology, vol. 315, no. 4, pp. 911–925, 2002.
- A. Obrdlik and P. Percipalle, “The F-actin severing protein cofilin-1 is required for RNA polymerase II transcription elongation,” Nucleus, vol. 2, no. 1, pp. 72–79, 2011.
- Y. Meng, Y. Zhang, V. Tregoubov et al., “Abnormal spine morphology and enhanced LTP in LIMK-1 knockout mice,” Neuron, vol. 35, no. 1, pp. 121–133, 2002.
- Y. Meng, H. Takahashi, J. Meng et al., “Regulation of ADF/cofilin phosphorylation and synaptic function by LIM-kinase,” Neuropharmacology, vol. 47, no. 5, pp. 746–754, 2004.
- K. Kousaka, H. Kiyonari, N. Oshima et al., “Slingshot-3 dephosphorylates ADF/cofilin but is dispensable for mouse development,” Genesis, vol. 46, no. 5, pp. 246–255, 2008.
- M. Schiffer, M. Bitzer, I. S. D. Roberts et al., “Apoptosis in podocytes induced by TGF-β and Smad7,” Journal of Clinical Investigation, vol. 108, no. 6, pp. 807–816, 2001.
- M. Schiffer, L. E. Schiffer, A. Gupta et al., “Inhibitory Smads and TGF-β signaling in glomerular cells,” Journal of the American Society of Nephrology, vol. 13, no. 11, pp. 2657–2666, 2002.
- D. T. Wu, M. Bitzer, W. Ju, P. Mundel, and E. P. Böttinger, “TGF-β concentration specifies differential signaling profiles of growth arrest/differentiation and apoptosis in podocytes,” Journal of the American Society of Nephrology, vol. 16, no. 11, pp. 3211–3221, 2005.
- N. Nomura, M. Nomura, K. Sugiyama, and J. I. Hamada, “Phorbol 12-myristate 13-acetate (PMA)-induced migration of glioblastoma cells is mediated via p38MAPK/Hsp27 pathway,” Biochemical Pharmacology, vol. 74, no. 5, pp. 690–701, 2007.
- S. Mowla, R. Pinnock, V. D. Leaner, C. R. Goding, and S. Prince, “PMA-induced up-regulation of TBX3 is mediated by AP-1 and contributes to breast cancer cell migration,” Biochemical Journal, vol. 433, no. 1, pp. 145–153, 2011.
- H. Kawamura, K. Yokote, S. Asaumi et al., “High glucose-induced upregulation of osteopontin is mediated via Rho/Rho kinase pathway in cultured rat aortic smooth muscle cells,” Arteriosclerosis, Thrombosis, and Vascular Biology, vol. 24, no. 2, pp. 276–281, 2004.
- F. R. Danesh, M. M. Sadeghit, N. Amro et al., “3-Hydroxy-3-methylglutaryl CoA reductase inhibitors prevent high glucose-induced proliferation of mesangial cells via modulation of Rho GTPase/p21 signaling pathway: implications for diabetic nephropathy,” Proceedings of the National Academy of Sciences of the United States of America, vol. 99, no. 12, pp. 8301–8305, 2002.
- V. Kolavennu, L. Zeng, H. Peng, Y. Wang, and F. R. Danesh, “Targeting of RhoA/ROCK signaling ameliorates progression of diabetic nephropathy independent of glucose control,” Diabetes, vol. 57, no. 3, pp. 714–723, 2008.
- F. Peng, D. Wu, B. Gao et al., “RhoA/rho-kinase contribute to the pathogenesis of diabetic renal Disease,” Diabetes, vol. 57, no. 6, pp. 1683–1692, 2008.
- R. Komers, T. T. Oyama, D. R. Beard, and S. Anderson, “Effects of systemic inhibition of Rho kinase on blood pressure and renal haemodynamics in diabetic rats,” British Journal of Pharmacology, vol. 162, no. 1, pp. 163–174, 2011.
- D. Chhabra and C. G. Dos Remedios, “Cofilin, actin and their complex observed in vivo using fluorescence resonance energy transfer,” Biophysical Journal, vol. 89, no. 3, pp. 1902–1908, 2005.
Copyright © 2012 Beina Teng et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.