Synergistic Effect of Hyperglycemia and
Suppression on Adult Mouse Islet Beta Cell Replication
Figure 3
Long-term ex vivo effect of p27Kip1 silencing on the blood glucose level (a) and body weight (b) in a syngeneic mouse transplantation model. Each mouse with streptozotocin-induced diabetes was transplanted with 200 mouse islets transduced with either shRNAs to p27kip1or the non-target shRNA control under the left renal capsule at day 0. After transplantation, blood glucose level and body weight were measured. At 84 days following transplantation, the graft-bearing kidneys of all mice were removed by nephrectomy (solid arrow), and all mice were kept alive for 2 weeks before being sacrificed. In Figures 3(a) and 2(b), *, † indicates the marked mean compared to that on day 84 in the same group using paired Student’s t-test.
