| Image processing software | Detail | References |
| ImageJ | version 1.29, Natl. Inst. of Health, Bethseda, Md., U.S.A | Lassoued et al., [3] | Bajd and Serša, [21] | Tlapale-Valdivia et al., [38] | http://rsb.info.nih.gov/ij or https://imagej.net/ | Pérez-Nieto et al., [17] | Curic et al., [48] | Scheuer et al., [67] |
| SigmaScan®Pro | Version:5.50.4522.1800IC by Drug discovery Online | Romano et al., [12] Tlapale-Valdiviaet al., [38] | http://www.sigmaplot.co.uk/products/sigmascan/sigmascan.php | Angioloni and Collar [16] |
| MATLAB | The MathWorks Inc., | Gonzales-Barron and Butler [13] | Bajd and Serša, [21] | Natick, MA, USA | Shehzad et al.,[22] | Rouillé et al.,[30] | https://in.mathworks.com/ | Verdú et al., [18] | Eduardo, Svanberg and Ahrné [68] |
| Gebäckanalyse | Ver 1.3c 1997/98 program (Hochschule Ostwestfalen Lippe, Germany | Onyango, Unbehend and Lindhauer [69] |
| Labview | Vision Assistant 2009, National Instruments, USA | Che Pa et al., [5] |
| UTHSCSA | Version 2.0, University of Texas Health Science Centre, San Antonio, Texas | Skendi et al., [70] | ImageTool programme |
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