Ultrasound-Assisted Extraction of Bioactive Compounds from Annatto Seeds, Evaluation of Their Antimicrobial and Antioxidant Activity, and Identification of Main Compounds by LC/ESI-MS Analysis

Table 2

ANOVA table for the response variables for the UAE of bioactive compounds from annatto seeds.

Variable	UAE
	Polyphenol; (mg AG/g seed)	Bixin (%)
	p-value	p-value
Model	< 0.0001	< 0.0001
X1-Treatment time (min)	0.050	0.006
X2-pH	0.050	0.065
X3- Solvent concentration (%)	0.0001	< 0.0001
X4- Seed-to-solvent ratio (1:X4)	< 0.0001	0.003
X3X4	0.050	< 0.0001
X4X1	0.036	0.023
Lack of Fit	0.058	0.057
r^2	0,768	0,897
r^2-adj	0,731	0,872

AG: gallic acid.