Ultrasound-Assisted Extraction of Bioactive Compounds from Annatto Seeds, Evaluation of Their Antimicrobial and Antioxidant Activity, and Identification of Main Compounds by LC/ESI-MS Analysis

Table 3

Predicted local maximum in the optimization of the UAE applying a Box-Behnken experimental design.

Parameter	pH	Solvent concentration of the (%)	Seed-to-solvent ratio (1:X4)	Treatment time (min)	Polyphenol content; (mg AG/g seed)	Bixin; (%)
Predicted	7.5	95.98	7.82	30.00	2.71	0.43
Experimental	7.0	96.00	7.00	20.00	3.81	0.62
	Relative error				-1.10	-0.19
	Absolute error (%)				40.74	44.42

Values are expressed as mean ± standard deviation (n = 3). AG: gallic acid.