In Vitro Antioxidant and Cancer Inhibitory Activity of a Colored Avocado Seed Extract
Figure 3
Induction of cell cycle arrest and apoptosis in LNCaP cells by CASE treatment. Cells were treated with CASE for 12 h. (a) Cells were fixed in methanol and stained with propidium iodide, and cell cycle analysis was performed using flow cytometry. (b) After 12 h treatment, the effect of CASE on the expression of cyclins was determined by western blot. Cyclin expression was normalized to expression of β-actin. Following treatment with CASE for 12 h, (c) induction of apoptosis was determined by quantifying externalization of phosphatidylserine using flow cytometry and (d) determining the extent of caspase 3 and PARP cleavage using western blot. Cleaved caspase-3 and PARP levels were normalized to β-actin. The blots shown are representative of three independent experiments. The bar graphs represent the mean of three independent experiments ±SEM.