Validation of a Simple and Robust Liebermann–Burchard Colorimetric Method for the Assay of Cholesterol in Selected Milk Products in Ghana

<table class="table-group" id="tab4"><tr><td><table class="table"><tr><td class="thead-hr" colspan="4"><hr/></td></tr><tr class="thead"><td class="align_left">Test concentration (mg/ml)</td><td class="align_center">Mean% recovery ± SD</td><td class="align_center">Test concentration (mg/ml)</td><td class="align_center">Purity estimate from analysis</td></tr><tr><td class="thead-hr" colspan="4"><hr/></td></tr><tr><td class="align_left">0.032</td><td class="align_center">102.0 ± 0.75889</td><td class="align_center">0.032</td><td class="align_center">96.88 ± 0.7221</td></tr><tr><td class="align_left">0.040</td><td class="align_center">100.9 ± 1.017</td><td class="align_center">0.040</td><td class="align_center">95.97 ± 1.016</td></tr><tr><td class="align_left">0.048</td><td class="align_center">102.1 ± 0.6654</td><td class="align_center">0.048</td><td class="align_center">96.65 ± 0.7527</td></tr><tr><td class="align_left" colspan="4"><hr/></td></tr><tr><td class="align_left"><i>Acceptance Criteria</i></td><td class="align_center">[98%–102%]</td><td class="align_center"><i>True assay value (by manufacturer)</i></td><td class="align_center">95%</td></tr><tr class="table-tr"><td colspan="4"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

<div>Results showing accuracy of results from the developed method.</div>

International Journal of Food Science

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Table 4

Table 4: Validation of a Simple and Robust Liebermann–Burchard Colorimetric Method for the Assay of Cholesterol in Selected Milk Products in Ghana 