Methanol Extract of Coleus amboinicus (Lour) Exhibited Antiproliferative Activity and Induced Programmed Cell Death in Colon Cancer Cell WiDr

Table 1

Primer sequences apoptosis related genes.

Gene	Forward and reverse primer
BCL2 F ^∗	5′- GCT CTA AAA TCC ATC CAG -3′
BCL2 R ^∗	5′- CCT CTC CAT CAT CAA CTT -3′
BAX F ^∗∗	5′- CCC GAG AGG TCT TTT TCC GAG -3′
BAX R ^∗∗	5′- CCA GCC CAT GAT GGT TCT GAT -3′
Caspase 8 F ^∗	5′- AGA GTC TGT GCC CAA ATC AAC -3′
Caspase 8 R ^∗	5′- GCT GCT TCT CTC TTT GCT GAA -3′
Caspase 1 F ^∗∗	5′- CCT TCA AGG ACC TTG TCT GTT TAG -3′
Caspase 1 R ^∗∗	5′- GAG GGA TTA CCC AAC TGT GAG -3′
Caspase 9 F ^∗∗	5′- GAA TGA CGT GAA ACA CGA CAG -3′
Caspase 9 R ^∗∗	5′- TTA ACG GCA TCC CCC ACT TAG -3′
Caspase 7 F ^∗∗	5′- GCAGCGCCGAGACTTTTAG-3′
Caspase 7 R ^∗∗	5′- GCTGCAGTTACCGTTCCCAC-3′
TP53 F ^∗∗	5′ - CCG CAG TCA GAT CCT AGC -3′
TP53 R ^∗∗	5′- AAT CAT CCA TTG CTT GGG ACG -3′
ACTB F ^∗∗	5′- AGAGCTACGAGCTGCCTGAC -3′
ACTB R ^∗∗	5′- AGCACTGTGTTGGCGTACAG -3′

References: ^∗ [54]. ^∗∗ [10].