Methanol Extract of Coleus amboinicus (Lour) Exhibited Antiproliferative Activity and Induced Programmed Cell Death in Colon Cancer Cell WiDr

Table 2

The expression of apoptosis-related genes after cells treated with 5-FU and methanol extract of CA at 10, 15, 25, and 50 µg/ml concentrations.

Treatment	Fold change
	Bax	Bcl-2	p53	Caspase 8	Caspase 9	Caspase 1	Caspase 7
Control	1.000 ± 0.000^a	1.000 ± 0.000^a	1.000 ± 0.000^a	1.000 ± 0.000^a	1.000 ± 0.000^a	1.000 ± 0.000^a	1.000 ± 0.000^a
5-FU	1.800 ± 0.170^b	0.236 ± 0.025^b	3.285 ± 1.135^a	0.325 ± 0.005^b	4.785 ± 0.545^b	0.445 ± 0.015^a	0.235 ± 0.005^a
10 µg/ml	1.476 ± 0.138^b	0.054 ± 0.010^b	1.388 ± 0.402^a	0.788 ± 0.095^b	1.023 ± 0.412^a	0.931 ± 0.084^a	0.693 ± 0.082^a
15 µg/ml	1.665 ± 0.035^b	0.807 ± 0.062^b	1.887 ± 0.355^a	0.710 ± 0.000^b	1.263 ± 0.157^a	1.130 ± 0.024^a	1.020 ± 0.109^a
25 µg/ml	0.406 ± 0.023^b	0.083 ± 0.013^b	0.167 ± 0.001^a	0.080 ± 0.029^b	0.824 ± 0.119^a	42.421 ± 2.057^b	40.855 ± 7.147^b
50 µg/ml	0.773 ± 0.078^a	0.043 ± 0.008^b	0.050 ± 0.008^a	0.046 ± 0.001^b	0.825 ± 0.111^a	34.253 ± 9.917^b	37.888 ± 11.806^b

^a versus control.^b versus control.