Research Article

Alleviating Chilling Injury in Stored Pomegranate Using a Single Intermittent Warming Cycle: Fatty Acid and Polyamine Modifications

Table 2

The content of malondialdehyde and fatty acids in pomegranate fruit peel under one intermittent warming cycle during 70 days of cold storage.

MDA
(μmol g-1 fresh weight)
SFAs
(mg 100 g-1 fresh weight)
MUFAs
(mg 100 g-1 fresh weight)
PUFAs
(mg 100 g-1 fresh weight)
C10C12C14C15C16C17C18C16:1C18:1C18:2C18:3

At harvest18.60 ± 0.741.46 ± 0.115.36 ± 0.208.67 ± 0.451.49 ± 0.1316.39 ± 0.661.92 ± 0.2712.10 ± 1.5317.78 ± 1.0711.26 ± 1.6312.44 ± 0.6717.75 ± 1.47
Treatments
15NI13.44 ± 0.17g1.35 ± 0.09bc5.02 ± 0.06cd8.18 ± 0.14cd1.36 ± 0.14bc15.47 ± 0.37de1.83 ± 0.9bc11.37 ± 0.49cde18.56 ± 0.49a11.74 ± 0.60ab12.99 ± 0.77a18.56 ± 0.59a
15WI10.22 ± 0.16h1.30 ± 0.19c4.77 ± 0.21d7.81 ± 0.56d1.30 ± 0.13c14.76 ± 0.85e1.73 ± 0.16c10.85 ± 0.75e18.63 ± 0.77a11.79 ± 0.69ab13.04 ± 0.71a18.65 ± 0.63a
15NE35.93 ± 1.69a1.82 ± 0.07a6.72 ± 0.19a10.98 ± 0.41a1.80 ± 0.05a20.78 ± 0.59a2.41 ± 0.16a15.29 ± 0.39a12.97 ± 0.33e8.25 ± 0.37g9.13 ± 0.22d12.92 ± 0.46d
15WE19.22 ± 0.58e1.48 ± 0.21b5.40 ± 0.38cd8.83 ± 0.58c1.47 ± 0.13bc16.70 ± 0.52c1.96 ± 0.17b12.27 ± 0.52c18.40 ± 0.56a11.66 ± 0.46ab12.89 ± 0.82a18.41 ± 0.80a
25NI15.21 ± 0.57fg1.37 ± 0.13bc5.01 ± 0.44cd8.21 ± 0.79cd1.37 ± 0.16bc15.50 ± 1.06de1.82 ± 0.12bc11.40 ± 0.91cde18.90 ± 1.01a11.95 ± 0.69a13.21 ± 0.68a18.88 ± 0.71a
25WI16.74 ± 0.62f1.40 ± 0.13bc5.16 ± 0.41cd8.45 ± 0.44cd1.40 ± 0.17bc15.94 ± 0.69cd1.87 ± 0.11bc11.72 ± 0.88cde18.45 ± 0.28a11.67 ± 0.60ab12.91 ± 0.90a18.44 ± 0.50a
25NE35.34 ± 1.19a1.81 ± 0.08a6.69 ± 0.22a10.99 ± 0.33a1.83 ± 0.09a20.74 ± 0.66a2.42 ± 0.11a15.18 ± 0.40a12.98 ± 0.47e8.20 ± 0.33g9.03 ± 0.30d12.95 ± 0.25d
25WE19.95 ± 0.25e1.36 ± 0.04bc5.01 ± 0.29cd8.74 ± 0.08c1.49 ± 0.02b16.31 ± 0.09cd1.92 ± 0.04bc11.97 ± 0.35cd17.22 ± 0.23b11.03 ± 0.13bc12.31 ± 0.09a17.09 ± 0.58b
35NI23.84 ± 1.01d1.39 ± 0.09bc5.54 ± 0.35bc8.46 ± 0.38cd1.40 ± 0.09bc15.97 ± 0.45cd1.87 ± 0.09bc11.75 ± 0.52cde16.25 ± 0.38c10.28 ± 0.36cd11.36 ± 0.55b16.23 ± 0.51b
35WI24.11 ± 0.81d1.38 ± 0.07bc5.15 ± 0.41cd8.45 ± 0.37cd1.39 ± 0.10bc15.96 ± 0.76cd1.86 ± 0.06bc11.69 ± 0.62cde16.18 ± 0.45c10.24 ± 0.61cde11.32 ± 0.42b16.17 ± 0.42b
35NE35.72 ± 1.22a1.81 ± 0.07a6.71 ± 0.34a10.97 ± 0.40a1.84 ± 0.10a20.70 ± 0.48a2.43 ± 0.10a15.27 ± 0.49a12.96 ± 0.53e8.26 ± 0.31g9.06 ± 0.24d13.07 ± 0.31d
35WE29.36 ± 0.86c1.37 ± 0.09bc4.93 ± 0.48cd8.09 ± 0.46cd1.37 ± 0.09bc15.26 ± 0.53de1.78 ± 0.08bc11.22 ± 0.42de14.95 ± 0.40d9.48 ± 0.36ef10.47 ± 0.31bc14.97 ± 0.65c
45NI33.21 ± 1.99b1.71 ± 0.07a6.31 ± 0.51a10.33 ± 0.41ab1.72 ± 0.07a19.52 ± 0.56b2.30 ± 0.21a14.30 ± 0.45ab13.74 ± 0.72e8.72 ± 0.61fg9.64 ± 0.62cd13.77 ± 0.73d
45WI27.55 ± 1.31c1.35 ± 0.05bc4.98 ± 0.39cd8.17 ± 0.49cd1.36 ± 0.05bc15.43 ± 0.52de1.81 ± 0.07bc11.34 ± 0.55cde15.14 ± 0.54d9.59 ± 0.41de10.60 ± 0.64b15.15 ± 0.55c
45NE35.87 ± 2.46a1.82 ± 0.08a6.70 ± 0.60a10.97 ± 0.69a1.82 ± 0.10a20.73 ± 1.32a2.43 ± 0.26a15.24 ± 0.73a12.97 ± .67e8.22 ± 0.64g9.08 ± 0.32d12.98 ± 0.50d
45WE32.85 ± 2.07b1.66 ± 0.07a6.18 ± 0.42ab10.11 ± 0.50b1.68 ± 0.08a19.10 ± 0.59b2.24 ± 0.09a14.05 ± 0.40b13.82 ± 0.81e8.76 ± 0.38fg9.68 ± 0.62cd13.83 ± 0.65d
LSD2.1090.1610.6490.7830.1781.1120.2000.9940.8580.7930.9160.928

Fruit were stored at 2 ± 0.5°C and 90 ± 5% relative humidity. Intermittent warming cycle was 1 day at 20°C with 70% relative humidity before returning the treated fruit to storage. Temporal points of interruption were 15th, 25th, 35th, or 45th days of storage. WI stands for warmed fruit which were immediately sampled after warming on the date of interruption, NI stands for not warmed fruit which were sampled on the date of interruption, WE stands for warmed fruit, which were returned to storage and sampled at the end of the storage period, and NE stands for not warmed fruit sampled at the end of the storage period. MDA: malondialdehyde; SFAs: saturated fatty acids; MUFAs: monounsaturated fatty acids; PUFAs: polyunsaturated fatty acids; C10: Capric acid; C12: Lauric acid; C14: Myristic acid; C15: Pentadecylic acid; C16: Palmitic acid; C17: Margaric acid; C18: Stearic acid; C16:1: Palmitoleic acid; C18:1: Oleic acid; C18:2: Linoleic acid and C18:3: Linolenic acid. Data are means of 3 replicates ± standard deviation. Means within a column followed by the same letter are not significantly different by least significant difference (LSD) test, P <0.05.