Substantial Depletion of Vicine, Levodopa, and Tyramine in a Fava Bean Protein-Based Nutritional Product

Table 1

HPLC instrumentation and parameters.

Analyte(s)	Vicine, convicine, divicine, and isouramil in FBPI	Vicine in NP	Levodopa	Tyramine
LC system	Agilent Model 1260 (Agilent Technologies, Wilmington, DE, USA)
Detector	Agilent G4212B diode array		Agilent G1321A fluorescence
Detection wavelengths	276 nm, 214 nm, 273 nm, 278 nm, 283 nm	276 nm, 214 nm
LC column	YMC-Pack ODS-AQ^a	Zorbax Eclipse Plus C18^b	Zorbax Eclipse Plus C18^b	YMC-Pack ODS-AQ^a
Temperature	25°C	15°C	20°C	15°C
Mobile phase A	0.05 M KH2PO4, pH 2.9		1000/25 () 0.05 M KH2PO4, pH 2.9/ACN	0.02 M KH2PO4, pH 2.9
Mobile phase B	200/800 () H2O/ACN
Flow rate	0.5 mL/min	0.4 mL/min	0.4 mL/min	0.5 mL/min
Injection	10 μL	10 μL	5 μL	10 μL
Elution program	0% B 0-20 min, 100% B 20-25 min, 0% B 25-45 min (end)	0% B 0-25 min, 100% B 25-30 min, 0% B 30-45 min (end)	0% B 0-25 min, 100% B 25-30 min, 0% B 30-50 min (end)	0% B 0-5 min, 0-10% B 5-25 min (linear), 100% B 25-30 min, 0% B 30-45 min (end)

^a, 5 μm, 120 Å; Waters Corporation, Milford, MA, USA. ^b, 5 μm; Agilent Technologies, Wilmington, DE, USA.