Edible Oil Parameters during Deterioration Processes

Table 6

Advantages and disadvantages of the methods to measure antioxidant capacity.

Name	DPPH method	ABTS method	ORAC method	FRAP method
Radical or active species	Free radical 2,2-diphenyl-1-picrylhydrazyl.	Azinobis 3-ethylbenzothiazoline-6-sulfonic acid)	Peroxyl radicals	Fe^+3 2,4,6-tripyridyl-s-triazine (TPTZ)
Equipment	Spectrophotometer	Spectrophotometer	Fluorometer	Spectrophotometer
Advantage	Quick preparation	Detects hydrophilic and lipophilic antioxidants	It can detect antioxidant activity widely	Higher absorbance spectrum than the other methods
Disadvantages	The results vary according to the concentration of the free radical.	It is not a physiological radical, so it requires more preparation time	It must be used in in vitro samples	It is less accurate than the other methods, since the reducing power is not always antioxidant capacity.
Applications	Antioxidant activity in unrefined oils and native species. [87, 95].	Food industry	Food industry, biological fluids [97]	Food industry, botany, medicinal [104]