Use of α-Lactalbumin and Caseinoglycomacropeptide as Biopeptide Precursors and as Functional Additives in Milk Beverages Fermented by L. helveticus

Table 1

Sequences of biopeptides detected in the hydrolysates.

Source of peptides (type of analyzed hydrolysate)	L. helveticus strain	Identified peptide sequence	Mass (Da)	ID of the bioactive peptide in the database
CGMP	T105	LPYPYY	814.30	biopep00859/BioPepDB^b
CGMP	T80	LRF^a	434.26	biopep00880/BioPepDB
CGMP	T105	DKIHPF	755.39	biopep00136/BioPepDB; 7493/BIOPEP-UWM^c
CGMP	T105	AVPYPQR	829.44	biopep00091/BioPepDB; biopep04785/BioPepDB; biopep04775//BioPepDB; 3480/BIOPEP-UWM; 7875/BIOPEP-UWM
CGMP	DSMZ, T80, T105	DKIHP	608.33	biopep00135/BioPepDB
CGMP	T80	EMPFPKYPVEP	1332.64	biopep00178/BioPepDB
CGMP	B734	RPKHPI	746.45	biopep01209/BioPepDB
CGMP	B734	PYVRYL	809.44	biopep01134/BioPepDB
CGMP	B734	LTLTDVE	789.41	biopep00907/BioPepDB; 8155/BIOPEP-UWM
CGMP	T80	KYPVEPFTESQSLTL	1737.88	biopep00721/BioPepDB
α-la	DSMZ
CGMP	B734	GPFPIIV	741.44	8159/BIOPEP-UWM; biopep00385/BioPepDB
CGMP	T105	FVAPFPEVFGK	1236.65	biopep00304/BioPepDB
CGMP	B734, T80	IPNPIGSE	825.42	biopep00561/BioPepDB
CGMP	B734, DSMZ, T105	ARHPHPHLSF	1197.61	biopep00073/BioPepDB
CGMP	B734, DSMZ, T80, T105	SPPEIN	655.30	biopep01254/BioPepDB
α-la	T105	YQKFPQY	972.47	biopep01624/BioPepDB; 9254/BIOPEP-UWM
CGMP; α-la	B734, DSMZ, T80, T105	ERF^a	450.22	biopep00189/BioPepDB
CGMP	B734, DSMZ, T80, T105	VRSP	457.26	biopep01460/BioPepDB; 8309/BIOPEP-UWM
α-la	T80
CGMP	T80, T105, B734,	SRY^a	424.21	biopep01260/BioPepDB
α-la	T80, T105, B734, DSMZ
CGMP; α-la	B734, DSMZ, T80, T105	RPKHPIKHQGLPQEVLNEN	2233.22	biopep01215/BioPepDB
α-la	B734	RPKHPIKH	1011.60	biopep01211/BioPepDB
CGMP	T105	RPKHPIKHQGLPQEV	1762.99	biopep01214/BioPepDB
CGMP	B734, DSMZ, T105	RPKHPIKHQGLPQ	1534.88	biopep01213/BioPepDB
CGMP	B734, DSMZ, T105	RPKHPIKHQ	1139.6	7483/BIOPEP-UWM; biopep01212/BioPepDB
α-la	B734, T80, T105
CGMP	B734, T80, DSMZ, T105	HKEMPFPKYPVEPF	1744.86	biopep00457/BioPepDB
CGMP	B734, T105	MAIPPK	655.37	biopep00945/BioPepDB; 3293/BIOPEP-UWM
CGMP	B734, T80, DSMZ, T105	MAIPPKK	783.46	biopep00946/BioPepDB; 3294/BIOPEP-UWM^c
CGMP	B734, T80	IPNPIGSE	825.42	biopep00561/BioPepDB
CGMP	B734, T105	RELEE	674.32	biopep01181/BioPepDB
CGMP	T80, T105	QKAVPYPQRDMPI	1541.80	biopep01145/BioPepDB
CGMP; α-la	B734, T80, DSMZ, T105	AYPS	436.20	8472/BIOPEP-UWM; 8380/BIOPEP-UWM
CGMP	B734, DSMZ	LLR^a	400.28	8484/BIOPEP-UWM; biopep00827/BioPepDB
CGMP	T80, DSMZ	RELEELNVPGEIVESLSSSEESITR	2801.39	biopep04772/BioPepDB; 3055/BIOPEP-UWM
CGMP; α-la	B734, T80, DSMZ, T105	YQEPVLGPVRGPFPIIV	1880.06	biopep04801/BioPepDB; biopep04091/BioPepDB; biopep01621/BioPepDB
CGMP	B734, DSMZ T105	NLHLPLP	802.47	2669/BIOPEP-UWM; biopep01010/BioPepDB
CGMP; α-la	B734, T80, DSMZ, T105	VTSTAV	576.30	7481/BIOPEP-UWM; biopep01475/BioPepDB
CGMP; α-la	B734, T80, DSMZ, T105	LLYQEPVLGPVRGPFPIIV	2106.22	8174/BIOPEP-UWM
α-la	T105, DSMZ,	LLYQEP	761.40	biopep00829/BioPepDB
CGMP	DSMZ	YPFPGPI	789.41	biopep04798/BioPepDB; 7665/BIOPEP-UWM; 3262/BIOPEP-UWM
CGMP; α-la	B734, T80, DSMZ, T105	MAIPPKKNQDKTEIPTINTIASGEPTSTPTTEAVESTVATLEDSPEVIESPPEINTVQVTSTAV	6703.37	biopep03480/BioPepDB
CGMP; α-la	DSMZ	YYQQKP	825.40	8383/BIOPEP-UWM
CGMP	DSMZ	YIPIQY	795.42	biopep01570/BioPepDB; 7630/BIOPEP-UWM
CGMP	B734, T80, DSMZ, T105	MAIPPKKNQDK	1268.69	biopep04784/BioPepDB; 2796/BIOPEP-UWM; 3292/BIOPEP-UWM
CGMP	T105	VLPVPQK	779.49	biopep01419/BioPepDB; 7877/BIOPEP-UWM
CGMP	B734, T80, DSMZ, T105	VPSERYL	862.45	9250/BIOPEP-UWM; biopep01442/BioPepDB
CGMP	T80	VVPP	410.25	biopep01483/BioPepDB; 8308/BIOPEP-UWM
α-la	T105	TKKTKLTEEEKNRL	1716.97	biopep01292/BioPepDB
CGMP	B734, T80,
CGMP	B734, T80, DSMZ, T105	VQVTSTAV	803.40	biopep01445/BioPepDB; 8264/BIOPEP-UWM
CGMP	B734, T80	RPKHPIKHQGLPQEVLNENLLRF	2762.54	biopep03712/BioPepDB
α-la	B734

^aBased on accurate mass matching, <5 ppm; ^bdatabase: http://bis.zju.edu.cn/biopepdbr/ [33]; ^cdatabase: http://www.uwm.edu.pl/biochemia/index.php/pl/biopep [29].