Folin-Ciocalteu Reaction Alternatives for Higher Polyphenol Quantitation in Colombian Passion Fruits

Table 1

Standard reference compounds analyzed by UHPLC/MS and calculated polyphenol concentration in the three Passiflora acetone (70%) extracts. Compound found in extracts. LOQ, limit of quantitation 0.05 μg/mL. LOD, limit of detection, concentrations below 0.02 μg/mL.

Reference standards	Retention time (min)	[M+H]^+; (m/z)	P. edulis Flavicarpa (Maracuyá)	P. edulis Sims (Gulupa)	P. ligularis Juss (Granadilla)
Theobromine	3.63	181.07109	0.09	<LOQ	<LOQ
Theophylline	3.78	181.07291	<LOD	<LOD	<LOD
Epigallocatechin	3.80	307.07966	<LOQ	<LOQ	0.09
Cyanidin 3-rutinoside	3.90	595.16072	0.60	<LOQ	<LOQ
(+)-catechin	3.97	291.08484	0.29	0.28	<LOQ
Pelargonidin 3-glucoside	4.06	433.11133	<LOD	<LOD	<LOD
Caffeine	4.09	195.08672	<LOQ	<LOQ	<LOQ
Caffeic acid	4.10	181.04859	<LOD	<LOD	<LOD
(-)-Epicatechin	4.13	291.08776	0.18	0.22	0.05
Epigallocatechin gallate	4.15	459.08990	<LOQ	<LOQ	0.07
Vanillic acid	4.16	169.04865	<LOD	<LOD	<LOD
Quercetin 3-glucoside	4.45	465.09987	0.09	<LOQ	<LOQ
Epicatechin gallate	4.49	443.09508	<LOD	<LOD	<LOD
Cyanidin	4.55	287.05416	<LOQ	<LOQ	<LOQ
Kaempferol 3-glucoside	4.59	449.10558	<LOD	<LOD	<LOD
p-Coumaric acid	4.63	165.05377	<LOQ	<LOQ	<LOQ
Quercetin	4.63	303.04838	<LOD	<LOD	<LOD
Ferulic acid	4.71	195.06422	0.45	<LOQ	0.54
Rosmarinic acid	4.78	361.08999	<LOQ	0.13	<LOQ
Pelargonidin	4.82	271.05928	<LOQ	<LOQ	<LOQ
Luteolin	5.24	287.05356	<LOQ	<LOQ	<LOQ
Naringenin	5.49	273.07433	<LOD	<LOD	<LOD
Apigenin	5.53	271.05874	<LOQ	<LOQ	<LOQ
Kaempferol	5.57	287.05644	<LOD	<LOD	<LOD
Pinocembrin	6.16	257.07951	<LOD	<LOD	<LOD
Carnosic acid	7.94	333.20433	<LOD	<LOD	<LOD
Ursolic acid	9.46	457.36631	<LOQ	<LOQ	<LOQ
Calculated concentration			1.70 μg/mL	0.63 μg/mL	0.75 μg/mL