Research Article

Benzophenone Suppression of Quercetin Antioxidant Activity towards Lipids under UV-B Irradiation Regime: Detection by HPLC Chromatography

Table 1

(a) Rate constants for degradation of quercetin (QC) and benzophenone (BZP) (min−1) obtained from the slopes of the 1st order linear plots, ln = ( ), representing proportional decrease of QC and BZP concentrations as a result of the increasing UV-B irradiation periods. presents integrated areas of QC and BZP peaks, from the HPLC chromatograms (shown in Figures 2(a) and 3(a)) recorded at 371 and 250 nm, respectively. (b) Rate constants for the two peroxides formation, obtained from the slopes of the 1st order linear plot, ln = ( ), representing proportional increase of the two peroxides concentrations as a result of the increasing UV-B irradiation periods. presents integrated areas of the peroxides peaks (Per1 = 5.5 min, Per2 = 6.5 min), from the HPLC chromatograms (shown in Figure 4(a)) recorded at 234 nm.
(a) degrad., min−1

QC + PL90 + BZPQC + PL90BZP + PL90

QC
= 2.6 min
−0.03432−0.00332/
BZP
= 3.3 min
−0.00281/−0.01288

(b) Peroxides production, prod. Per, min−1

QC + PL90 + BZPQC + PL90BZP + PL90

Per1
= 5.5 min
0.01414−0.000090.01734
Per2
= 6.5 min
0.01756/0.01473