Journal of Diabetes Research

Journal of Diabetes Research / 2015 / Article

Research Article | Open Access

Volume 2015 |Article ID 587673 |

Mohsin Azam, Lindsey Marwood, Khalida Ismail, Tyrrell Evans, Sobha Sivaprasad, Kirsty Winkley, Stephanie Anne Amiel, "Diabetes Complications at Presentation and One Year by Glycated Haemoglobin at Diagnosis in a Multiethnic and Diverse Socioeconomic Population: Results from the South London Diabetes Study", Journal of Diabetes Research, vol. 2015, Article ID 587673, 8 pages, 2015.

Diabetes Complications at Presentation and One Year by Glycated Haemoglobin at Diagnosis in a Multiethnic and Diverse Socioeconomic Population: Results from the South London Diabetes Study

Academic Editor: Mitsuhiko Noda
Received08 Feb 2015
Revised12 Apr 2015
Accepted28 Apr 2015
Published18 May 2015


Background. WHO’s recommendation of  mmol/mol (6.5%) as diagnostic for type 2 diabetes mellitus (T2DM) was adopted by three UK London boroughs in May 2012. The South London Diabetes (SOUL-D) study has recruited people with newly diagnosed T2DM since 2008. We compared participants diagnosed before May 2012 with  mmol/mol to those with diagnostic  mmol/mol. Methods. A prospective cohort study of newly diagnosed T2DM participants from 96 primary care practices, comparing demographic and biomedical variables between those with diagnostic  mmol/mol or  mmol/mol at recruitment and after one year. Results. Of 1488 participants, 22.8% had diagnostic  mmol/mol. They were older and more likely to be white (). At recruitment and one year, there were no between-group differences in the prevalence of diabetic complications, except that those diagnosed with  mmol/mol had more sensory neuropathy at recruitment () and, at one year, had new myocardial infarction () but less microalbuminuria (). Conclusions. Use of  mmol/mol as the sole T2DM diagnostic criterion may miss almost a quarter of those previously diagnosed in South London yet  mmol/mol may not exclude clinically important diabetes.

1. Introduction

The use of plasma glucose, measured after fasting and a standardised oral glucose load, for the diagnosis of type 2 diabetes (T2DM) has long been considered inconvenient [1]. Glycated haemoglobin (), formed in a nonenzymatic reaction between glucose and haemoglobin and reflecting a 2-3-month average of plasma glucose concentrations in a single random sample [2], was considered as an alternative diagnostic tool for T2DM by an International Expert Committee in 2009 [1]. It has both advantages and disadvantages, [2] but in 2010 and 2011, respectively, both the American Diabetes Association (ADA) [3] and World Health Organisation (WHO) [4] proposed that  mmol/mol (6.5%) is diagnostic of T2DM, based on successive reproducible data from a number of publications linking concentrations and diabetes specific complications, notably retinopathy [58].

Prior to this, people in South London, in the United Kingdom [9], were diagnosed with diabetes by plasma glucose measurements, with or without formal glucose tolerance tests, although was measured at diagnosis for assessment and monitoring purposes. The authorities providing healthcare to a large area of South London did not adopt WHO’s recommendations of  mmol/mol (6.5%) as the diagnostic tool for T2DM until May 2012 [10]. Four years earlier, in May 2008, the South London Diabetes Study (SOUL-D) had begun recruiting adults with new-onset diabetes from 96 primary care practices in the London boroughs of Lambeth, Southwark, and Lewisham into an observational cohort study [11]. The majority were diagnosed prior to May 2012 using plasma glucose values. SOUL-D created an opportunity to examine how well would perform compared to previously used methods for diagnosing diabetes in this multiethnic, high-risk population.

The aims of this study were to examine the proportion of participants in the SOUL-D cohort that were diagnosed with  mmol/mol (6.5%) before the new guidelines were introduced; determine whether these participants were significantly different from participants with higher at diagnosis in their demography and diabetes complications; and investigate the criteria used to diagnose diabetes in patients with  mmol/mol (6.5%).

2. Patients and Methods

2.1. Subjects

One thousand seven hundred and fifteen people with newly diagnosed T2DM diagnosed before May 2012 were recruited from 96 (70% eligible) consenting primary care practices in the London boroughs of Lambeth, Southwark, and Lewisham, which serve a diverse population of almost one million residents. The ethnic origin of the boroughs’ population is 66.6% white, 20% black, and 13.4% South Asian [9]. Eligible participants were aged 18–75 years and had been diagnosed with T2DM within the last 6 months. Exclusion criteria included being a temporary resident, people diagnosed with diabetes other than T2DM, known severe mental illness (dementia, bipolar disorder, substance dependence, and personality disorder), severe advanced diabetes complications (e.g., being registered blind, requiring dialysis, or above knee amputation), and those not fluent in English (estimated at 7% [9]).

2.2. Methods

This was a prospective cohort study comparing 2 participant groups: those diagnosed with T2DM with  mmol/mol (6.5%) and those diagnosed with  mmol/mol (6.5%). The protocol for recruitment of participants in SOUL-D has been published previously [11], but, in brief, potential participants were identified by 6 monthly practice database reviews and invited to participate if they had a new diagnosis of T2DM.

Consenting patients attended their primary care practice to meet a SOUL-D researcher with whom they completed a standardised interview including medical history, employment status, education history (years of education attended), physical examination, and blood test. This was completed within 6 months of diagnosis (recruitment data) and 12 months after recruitment (one-year data). Additional clinical data from the time of diagnosis (diagnostic data), including , body mass index (BMI), blood pressure, urinary albumin : creatinine ratio (ACR), and lipid profile (low-density lipoprotein (LDL), total cholesterol, high-density lipoprotein (HDL), and triglycerides), were extracted from each participant’s medical records to give preintervention parameters. Cross-sectional analysis was undertaken to compare diagnostic and recruitment clinical and sociodemographic variables between those participants whose diagnostic had been <48 mmol/mol and those with higher diagnostic values. Analysis of year 1 study follow-up data was also undertaken to see if early progression of diabetes (reflected by and treatment prescribed) varied between groups.

For cross-sectional baseline (diagnostic and recruitment) analysis, age, gender, and self-reported ethnicity (using the 2001 consensus classification [9]) were noted at recruitment. Date of diabetes diagnosis and at diagnosis were recorded from medical records. Macrovascular complications, myocardial infarction (MI) and stroke/cerebrovascular accident (CVA), were defined by history given at recruitment and confirmed by examination of medical records. BMI (kg/m2), blood pressure (mmHg), foot examination, and sensory neuropathy were determined at recruitment, the last using vibration perception threshold >25 volts measured by neurothesiometer (Scientific Laboratory Supplies, Wilford, Nottingham). Retinopathy status was obtained from the Diabetes Eye Complications Screening (DECS) service, the local community based eye photography service used in all three boroughs, using digital two-field retinal photographs and the English Retinopathy Minimum grading [12]. Fasting blood was taken for and lipid measurements. Microalbuminuria was defined on a single ACR measurement in the records of >3 μg/mg. Medication status (presence or absence of oral hypoglycemic agents and insulin) at recruitment was recorded from patient history, confirmed from the participants’ medical records.

One year follow-up assessment was made within a 3-month window of 12 months after recruitment. The data collection made at recruitment was repeated. If participants declined, data were obtained from their GP surgery (with permission) within a three-month window of their study appointment.

To determine the basis of diagnosis for each participant whose was <48 mmol/mol (6.5%) at diagnosis, additional specific permission was granted to access the clinical database of 43 GP surgeries, selected for having ≥5 participants meeting the above criteria. Patient records were examined for details of symptoms at diagnosis (polyuria, polydipsia, fatigue, blurred vision, and weight loss) and details of diagnostic testing (fasting plasma glucose (FPG), random plasma glucose (RPG), or oral glucose tolerance test (OGTT) glucose concentrations) were noted. The data were compared with the relevant WHO recommendations to assess the accuracy of the original diagnosis [6]. Case-by-case judgements on the diagnosis were made by 2 independent clinicians reviewing the data.

(%) at diagnosis was measured in one of 3 local laboratories according to IFCC methods (aligned with the DCCT) based on HPLC and then quantified during capillary electrophoresis or electron spray ionization mass spectrometry. The assay methods used were (1) the Trinity Biotech Ultra 2 boronate affinity chromatography (coefficient variations (CV%) 0.82%, 0.91%, and 0.46% for normal, intermediate, and high values based on 20 assays with the same run time), (2) the Trinity Biotech Premier Hb9210 analyser, also a boronate affinity chromatography-based high performance liquid chromatography system (CV% 1.62%, 1.59%, and 1.68% for low, medium, and high values, resp.), and (3) TOSOH G7 ion exchange with imprecision CV% less than or equal to 1.2%. For all three laboratories, the CV% was well below the recommended upper limit of 2% CV and there were no changes in the methodologies between 2008 and 2013. samples measured at recruitment and one year were all measured in the central laboratory (method 1). Percentage (%) values were converted to mmol/mol by subtracting 2.15 then multiplying by 10.929 [13]. Lipid profiles and ACRs were measured using Siemens ADVIA 2400. The PEG-enhanced immunoturbidimetric assay was used for urinary albumin and the Jaffe reaction for urinary creatinine.

Statistical analysis was performed using SPSS version 22 [14]. Data are presented as mean [standard deviation (SD)] where data were normally distributed, or median [interquartile range (IQR)] where data were skewed, which could not be corrected by log transforming the variables, or as a count (percentage) for categorical variables, all stratified by diagnostic status. Unadjusted statistical analyses, comparing participants diagnosed with  mmol/mol (6.5%) to those with  mmol/mol (6.5%), were conducted using one-way ANOVA for normally distributed continuous data and Mann-Whitney analyses for nonnormally distributed continuous data (diagnostic variables: , total cholesterol, LDL, HDL, triglycerides, systolic and diastolic blood pressure, and BMI). Pearson chi-squared testing was used for comparisons of categorical data (ethnicity, age, gender, and the presence or absence of complications at recruitment and one year, as well as medication status). Binary logistic regression was performed to assess the association between demographic and diagnostic data (age at diagnosis, ethnicity, gender, diagnostic BMI, blood pressure, and lipid profile) and diagnostic category (< or ≥48 mmol/mol (6.5%)), accounting for multiple comparisons. Mann-Whitney test was used to compare the mean rate of change in glycaemia between both groups.

The study was approved by King’s College Hospital Research Ethics Committee (reference 08/H0808/1). All participants gave informed consent.

3. Results

Of 1805 participants recruited into SOUL-D, this analysis was restricted to 1715 participants diagnosed before May 2012 (Figure 1). Fifteen participants were excluded for failing to meet inclusion criteria or withdrawing themselves and 212 were excluded because was not documented at diagnosis. Of the remaining 1488, 55.11% were male (), mean age was years, and 50.13% (), 39.58% (), and 10.28% () were of white, black, and South Asian/other ethnicity, respectively. Age and gender were not significantly different in those participants without at diagnosis and the population diagnosed after May 2012 (all ).

Three hundred and thirty-nine participants (22.78%) had  mmol/mol (6.5%) at the time of diagnosis by glucose criteria. This group was significantly older ( versus ; ) but had no significant difference in gender split (, 55.46% versus 55.00% male for the <48 mmol/mol and ≥48 mmol/mol (6.5%) resp.) groups versus the higher group Table 1. People of white ethnicity were overrepresented, while people of black and South Asian/other ethnicity were underrepresented (). They had a significantly higher proportion of sensory neuropathy at recruitment but no significant differences in prevalence of retinopathy, microalbuminuria, MI, or stroke/CVA. In terms of nonglycemic cardiovascular risk at diagnosis, they had lower BMI and diastolic blood pressure, triglycerides, and total cholesterol ( for all) but no significant differences in systolic blood pressure, LDL-cholesterol, or HDL-cholesterol. All but 7 participants (4 with  mmol/mol (6.5%)) had information on symptoms at presentation, with significantly more participants being asymptomatic at diagnosis in the low group (68.96% versus 55.41%, ). At recruitment, the participants with low at diagnosis group were significantly less likely to be receiving oral hypoglycemic agents (29.1% versus 61.9%, ) with a trend for fewer receiving insulin (1.78% versus 3.76%, ). The group with low at diagnosis were more likely to be retired but there were no reported differences in proportion in full- or part-time employment or years of education attended (Table 2).

with dataHbA1c < 48 mmol/mol (6.5%)HbA1c ≥ 48 mmol/mol (6.5%) value

Demographical data
Age148858.73 (±10.36)54.87 (±11.07)<0.0001
Gender (% male)1488188 (55.46)632 (55.00)0.8829
Ethnicity (%)1488
 White218 (64.31)528 (45.95)<0.0001
 Black98 (28.91)491 (42.73)
 Asian23 (6.78)130 (11.31)

Median (IQR) HbA1c at diagnosis
HbA1c (mmol/mol)148844.26 (42.08–45.35),58.47 (51.91–82.52)<0.0010
HbA1c (%)6.20 (6.00–6.30)7.50 (6.90–9.70)

Cardiovascular risk factors (SD) at diagnosis
LDL-C (mmol/L)12112.86 (±0.98)3.67 (±21.83)0.5313
HDL-C (mmol/L)12511.28 (±0.67)1.24 (±0.44)0.1750
Total cholesterol (mmol/L)13574.70 (±1.32)4.90 (±1.50)0.0053
Triglycerides (mmol/L)12731.40 (±0.85)1.59 (±1.29)0.0001
BMI (kg/m2)138030.77 (±7.74)31.60 (8.40)0.0035
BPS (mmHg)1409133.96 (±16.84)134.65 (±16.28)0.5087
BPD (mmHg)140980.00 (±11.00)82.00 (±12.00)<0.0001

Complication status, HbA1c medication status at recruitment
Retinopathy (%)133530 (9.43)80 (7.86)0.3749
Microalbuminuria (%)123737 (12.76)158 (16.68)0.1279
Sensory neuropathy (%)136832 (10.16)70 (6.65)0.0393
MI (%)148224 (7.08)55 (4.81)0.1026
Stroke (%)14798 (2.37)40 (3.50)0.3042
HbA1c (mmol/mol)139542.08 (38.80–44.26)50.82 (45.35–60.65)<0.0001
HbA1c (%)6.00 (5.70–6.20)6.80 (6.30–7.40)
Receiving insulin (%)14816 (1.78)43 (3.76)0.0743
Receiving oral diabetes agents (%)147097 (29.1)704 (61.9)<0.0001

HbA1c ≥ 48 mmol/mol (6.5%)HbA1c < 48 mmol/mol (6.5%)

In full-time employment (%)430 (37.42)119 (35.10)
In part-time employment (%)123 (10.70)36 (10.62)
On sick leave (%)23 (2.00)9 (2.65)
Unemployed (%)189 (16.45)34 (10.03)
Medically retired (%)51 (4.44)18 (5.31)
Housewife/househusband (%)43 (3.74)6 (1.77)
Retired (%)289 (25.15)117 (34.51)

Education status
Years of education (mean ± SD)13.19 ± 2.9613.50 ± 3.15

, all other comparisons not significant.

When demographic and diagnostic data (age at diagnosis, ethnicity, gender, diagnostic BMI, blood pressure, and lipid profile) were entered into a multiple binary logistic regression, only being black (), South Asian/other (), age at diagnosis (), and triglyceride levels at diagnosis () were significant predictors of whether participants were diagnosed with  mmol/mol (6.5%) or ≥48 mmol/mol (6.5%). The resulting model accounted for 7% of the variance and correctly identified 98.5% of cases.

3.1. Year One Analysis (Table 3)

with dataHbA1c < 48 mmol/mol (6.5%)HbA1c ≥ 48 mmol/mol (6.5%) value

Median (IQR) HbA1c at year 1
HbA1c (mmol/mol)125143.17 (40.98–47.54),49.72 (45.35–57.37)<0.0001
HbA1c (%)6.10 (5.90–6.50)6.70 (6.30–7.40),

New complication status at year 1
Retinopathy (%)122032 (10.88)126 (13.61)0.2257
Microalbuminuria (%)102522 (9.21)123 (15.65)0.0123
MI (%)127810 (3.38)12 (1.22)0.0124
Stroke (%)12724 (1.4)7 (0.72)0.2951

Cumulative microalbuminuria and MI status at year 1
Microalbuminuria (%)121839 (14.77)191 (20.02)0.0540
MI (%)148729 (8.55)59 (5.14)0.0190

Medication status at year 1
Receiving oral agents (%)129692 (30.6)715 (71.9)<0.0001
Receiving insulin (%)12948 (2.67)37 (3.72)0.3817

Cardiovascular risk factor status (SD) at year 1
BMI (kg/m2)130831.28 (±6.44)32.14 (±6.35)0.0400
BPD (mmHg)128280.21 (±10.76)82.22 (±10.91)0.0057
BPS (mmHg)1284135.42 (±18.28)134.57 (±17.23)0.4614
LDL-C (mmol/L)11642.49 (±0.80)2.51 (±0.84)0.7485
HDL-C (mmol/L)12001.32 (±0.48)1.25 (±0.34)0.0081
Total cholesterol (mmol/L)12344.40 (±1.30)4.40 (±1.20)0.0942
Triglycerides (mmol/L)11642.49 (±0.80)2.51 (±0.84)0.5454
Smoking status (%)1149190 (21.62)41 (15.19)0.0211

Of the 1488 participants in the study, 21.51% () were not available for follow-up at year 1: 6 had died; 262 were not contactable; and 52 had withdrawn from the study.

remained significantly lower in those participants with  mmol/mol (6.5%) at diagnosis, (Table 3), and there was a significant difference in the change in from recruitment to year 1 between the two groups (). Participants with  mmol/mol (6.5%) at diagnosis showed a slight increase in (median (IQR): 1.09 (−1.09–4.37) mmol/mol or 0.10 (−0.10–0.40)%), compared to a slight fall overall in the group with the high value at diagnosis (0.00 (−5.4645 to 4.3716) mmol/mol and 0.00 (−0.5000 to 0.400)%). BMI and diastolic blood pressure as well as smoking status were significantly lower and HDL cholesterol was significantly higher in the low at diagnosis group ( for all), with no significant differences in systolic blood pressure, LDL-cholesterol, total cholesterol, and triglycerides between groups. A higher proportion of those with low at diagnosis reported a new MI (3.38% versus 1.22%, ). When the cumulative prevalence was compared between groups, that is, any MI events prior to year 1, the low group remained significantly more likely to have past MI (8.55% versus 5.14%, ). Numbers were very small, but, while there were no differences in a post hoc analysis between those with and without a history of MI at recruitment, three of those reporting new MI at year one were of black ethnicity and one was of Asian/other ethnicity, with none being white. Significantly fewer participants in the lower group had developed new microalbuminuria (9.21% versus 15.65%, ), and the cumulative prevalence of microalbuminuria prior to year 1 almost achieved significance (14.77% versus 20.02%, ). Incidence of new retinopathy and stroke did not differ significantly between groups (all ). A lower percentage of participants in the low at diagnosis group were receiving oral hypoglycemic agents (30.6% versus 71.9%, ) but the difference between groups in those receiving insulin at one year did not achieve statistical significance (2.16% versus 4.22%, ).

3.2. Review of Diagnosis in Low Diagnostic Group (Table 4)

Basis of diagnosisAsymptomatic and repeat test not performedIncorrectly diagnosed according to WHO

Fasting plasma glucose66 (37.71%)18 (27.27%)26 (39.39%)
OGTT68 (38.86%)21 (30.88%)34 (50.00%)
Random plasma glucose28 (16.00%)12 (42.86%)15 (53.57%)
HbA1c13 (7.42%)5 (38.46%)13 (100%)

Total (%)17563 (36.00%)89 (50.86%)

Information on diagnostic tests was obtained for 175 participants (51.62%) in the low at diagnosis group from 43 practices. These 175 participants were not significantly different from the remainder of the group in terms of demographical and biomedical data () for all factors studied. There were 38.86%, 37.71%, 16.00%, and 7.42% participants diagnosed on basis of 2-hour plasma glucose, fasting plasma glucose, random plasma glucose, and measurements below the WHO recommendation (median (and IQR) for 46.45 (45.35–46.45) mmol/mol or 6.40 (6.30–6.40)%). Sixty-three (36.0%) asymptomatic patients, for whom data were available, had no repeat or subsequent alternative test documented. Eighty-nine (50.86%) participants, for whom data were available, fell outside prevailing WHO criteria for T2DM diagnosis.

4. Discussion

The aims of this study were to estimate the proportion of individuals in an urban multiethnic cohort that might not be diagnosed with T2DM if  mmol/mol is the sole diagnostic criterion and assess whether individuals diagnosed on alternative criteria and not meeting the new criterion differed in terms of demographics and biomedical outcomes from those with at diagnosis. In our cohort, almost a quarter of people previously diagnosed with T2DM would not be deemed diabetic, where was the sole diagnostic criterion. These people were older at diagnosis and more likely to be of white ethnicity. They were also more likely to have been asymptomatic at diagnosis. At recruitment, however, there was no difference in their complication status except for a higher prevalence of sensory neuropathy. One year later, although fewer were prescribed medical therapy for hyperglycemia, their remained lower but more had an MI in the year, although the group had lower BMI, diastolic blood pressure, and triglycerides throughout and lower prevalence and development of microalbuminuria. There were no significant differences in other micro- and macrovascular complications.

To our knowledge, this is the first study that has looked at the phenotypes associated with the different criteria for diagnosing T2DM. Other studies have found sole use of  mmol/mol (6.5%) to diagnose T2DM can alter the epidemiology of T2DM, reporting that diagnostic misses 62–65% of people (especially asymptomatic) identified on OGTT in screening programmes [1517].

A high proportion of participants in both of our groups, significantly greater in those with low at diagnosis, denied osmotic symptoms at diagnosis. All diagnostic criteria require a second confirmatory biochemical test to establish the diagnosis in the absence of symptoms. Evidence of fulfilment of this requirement was absent from half the records investigated for this. Information not found in the patient or study records may have contributed to the diagnosis at the time. However, it is possible that in these participants another health event may have lowered physicians’ threshold for diagnosing diabetes (reverse causality) [18]. The overrepresentation of retired people in the group may be a reflection of the slightly increased age, and/or a greater engagement with healthcare procedures, including screening. While healthcare is free at the point of delivery in the UK, cultural issues may influence uptake, although it should be noted that neither employment status nor educational attainment was different between the groups. Even with the potential for misdiagnosis of diabetes in some participants with low at diagnosis, the lack of difference in diabetes complications suggests that opportunities for secondary prevention may be missed by widespread use of alone to diagnose diabetes, for example, in screening programmes.

Nonglycemic cardiovascular risk factors such as BMI, lipid profile, and diastolic blood pressure were reduced in the low at diagnosis group and prevalence of smoking was not different. Nevertheless, although absolute numbers are low, there was a higher incidence of new MI at year one (with a possible trend towards a greater positive history of MI at recruitment). Possible contributors to this occurrence may include the slightly greater age of the subjects in this group, or their different ethnicity (see below), or reverse causality, with presence of a cardiovascular risk event increasing the chance of being screened for diabetes. Sensory neuropathy was also higher at recruitment in the group with the low diagnostic . Sensory neuropathy is less specific to diabetes than retinopathy and nephropathy, although dysglycaemia has been implicated in the pathogenesis of otherwise idiopathic neuropathy [1922]. The higher proportion of individuals on oral hypoglycemic agents in the higher group at recruitment and one year mirrors their average at those times. Given the lack of difference in complication status found between T2DM participants diagnosed with below or above the currently recommended guideline, our findings underline the importance of also applying non- tests.

Only ethnicity, age at diagnosis, and triglyceride levels predicted diagnosis with below 48 mmol/mol (6.5%) in multivariate analysis. Ethnicity may contribute to the differences in BMI, LDL cholesterol, and diastolic blood pressure noted at diagnosis and even to the higher development of MI in the first year after recruitment in the low diagnostic group. The SOUL-D participants of black ethnicity still show the traditional “cardioprotective” lipid profile, but they show higher blood pressure compared to white participants [11], and they were underrepresented in the low diagnostic group, although unexpectedly the incident MI in the study occurred in nonwhite participants. The proportion of people of black ethnicity recruited to SOUL-D precisely matches the proportion in registers of people with existing diabetes, suggesting that the SOUL-D study did pick up a representative sample of all those at risk for type 2 diagnosis and again arguing against healthcare access as a major contributor to the differences observed.

It is however likely that ethnicity itself impacts on at presentation of diabetes [2325]. Higher in black people may not reflect higher plasma glucose concentrations and it has been suggested that plasma glucose might be more applicable than for diagnosing diabetes in black people [26]. However, two large population studies have shown that retinopathy prevalence is as high for any given and indeed may be higher than the reference category for a lower in black populations [27, 28]. This suggests is potentially stronger at predicting complications than plasma glucose and therefore a superior screening tool, as the main drive to diagnose asymptomatic diabetes is to engage in prevention of complications [27, 28]. Our finding of higher age at diagnosis in participants diagnosed with  mmol/mol (6.5%), despite positive correlations between age and [29, 30], may relate to the younger age at diagnosis of diabetes in nonwhite populations [13].

Limitations include the exclusion of individuals with very advanced complications and those who were housebound or not fluent English language speakers. Exclusion of those with very advanced complications may have been greater in the higher group, although very advanced complications are not common in the newly diagnosed patients who were the target of this study. Nonfluent English language speakers might also be at higher risk for worse disease in that they may be less likely to access English language health services; however they formed only 7% potentially eligible patients [13]. The power of comparisons in biomedical data between diagnostic groups was weakened by a low number of micro- and macrovascular events in both groups. Positive aspects of the study were the large sample size and high representation of participants of black ethnicity, reflecting engagement with the specific multicultural population and their high risk for T2DM. The availability of diagnostic data, while not measured in a core laboratory, was analysed using similar assay methods and has allowed us to look at the characteristics of the subjects before intervention.

In conclusion, almost a quarter of SOUL-D study participants may not have been diagnosed with T2DM had been introduced as the sole diagnostic tool before their diagnosis. This may be of particular importance to asymptomatic individuals, where the purpose of diagnosis is to avoid future diabetes-related complications. This study provides evidence for the consideration of ethnicity when diagnosing T2DM using : its use may miss people of white ethnicity who have previously been diagnosed on other criteria and may change the individuals identified with or without diabetes in screening programmes, perhaps particularly in older people and those of nonblack ethnicity. While long term follow-up of the cohorts reported here will be confirmatory, our present findings so suggest that while of 48 mmol/mol (6.5%) or greater as the diagnostic marker for T2DM is useful when positive, a negative result cannot be taken as conclusive evidence to exclude the diagnosis of clinically important diabetes.


CVA:Cerebrovascular incident
:Glycated haemoglobin
MI:Myocardial infarction
T2DM:Type 2 diabetes mellitus
ACR:Albumin creatinine ratio
BMI:Body mass index
OGTT:Oral glucose tolerance test.


The views expressed are those of the authors and not necessarily those of the NHS, the NIHR, or the Department of Health.

Conflict of Interests

The authors declare that there is no conflict of interests regarding the publication of this paper.


This paper presents independent research funded by the National Institute for Health Research (NIHR) under its Programme Grants for Applied Research Programme (Grant Reference no. RP-PG-0606-1142). The authors are grateful to all the general practitioners and their staff for the help provided in identifying and approaching patients and in making available facilities for conducting interviews. They would also like to thank their research team, past and present: J. Schonbeck, J. Valka, N. Iles, S. Brooks, J. Hunt, K. Twist, R. Stopford, G. Knight, A. Barlow, L. East, B. Jackson, E. Britneff, J. Pierre-Laake, C. Garrett, C. Mohandas, and A. Bayley. Finally, they are extremely grateful to the participants in SOUL-D without whom this study would not have been possible.


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Copyright © 2015 Mohsin Azam et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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