Table 2: Experimental design matrix by Box–Behnken for three level-three factors response surface study and responses.

Run
(°C)

(h)

(w/v)
Exp. yield
(%)
Pred.
yield
Exp. TPC
(mg AAE/g)
Pred.
TPC
Exp. TAA
(mg AAE/g)
Pred.
TAA

17.07475.00 ± 0.53476.8417.98 ± 1.0418.99
27.48 ± 0.0726.89164.13 ± 0.39165.0914.53 ± 0.5413.04
24.50 ± 0.2125.78420.00 ± 1.28421.9421.53 ± 0.3521.08
26.64 ± 0.1627.99172.14 ± 0.23173.1214.75 ± 1.0513.58
20.41 ± 0.2121.07185.08 ± 0.21185.0915.76 ± 0.9515.78
25.63 ± 0.0226.00304.10 ± 0.44305.2110.33 ± 0.2011.90
24.12 ± 0.1825.98268.32 ± 0.88267.0911.25 ± 0.6711.79
22.45 ± 0.3623.98406.33 ± 0.31405.0922.98 ± 0.0922.76
24.21 ± 0.0523.89464.36 ± 0.73465.0022.15 ± 0.1423.97
19.11 ± 0.1220.98263.80 ± 0.18264.0112.89 ± 0.0512.11
26.56 ± 0.5525.66167.97 ± 0.61166.9815.35 ± 0.0514.67
28.56 ± 0.2328.0097.25 ± 0.3396.9815.53 ± 1.0516.35
26.83 ± 0.2727.08218.39 ± 0.35217.0913.43 ± 0.2412.47
20.41 ± 0.0621.03268.64 ± 0.33267.9415.76 ± 0.9516.45
24.72 ± 0.0125.01439.12 ± 0.45438.9915.17 ± 0.4014.89

Note. Yield of extract, total polyphenol content (TPC), and total antioxidant activity (TAA) are represented as means ± standard deviation of triplicate determinations.