Research Article

Microwave Assisted Extraction of Defatted Roselle (Hibiscus sabdariffa L.) Seed at Subcritical Conditions with Statistical Analysis

Table 4

The results of experimental verification.

Extraction timeMicrowave powerSolvent/solid ratioExtraction yieldTotal phenolic contentTotal flavonoid content
(min)(W)(mL/g)(%)(mg GAE/g)(mg QE/g)

Predicted1030097.717860.621216.670212.2640
Actual1030097.713565.036718.22446.4524

The maximum temperature and pressure recorded are 163°C and 18 bar, respectively.