Review Article

Electrical Impedance Spectroscopy for Quality Assessment of Meat and Fish: A Review on Basic Principles, Measurement Methods, and Recent Advances

Table 2

Application of EIS for quality assessments of meat and fish.

ObjectQuality indicatorPredictor variableFrequencyAlgorithmAccuracyReference

Ageing state: beef
HeifersWBSFElectrical impedance(EI), conductivity(EC)A wide range for EI, 1 KHz for ECCA (EI), and −0.65 (EC)[63]
beefMechanical resistance1,100 kHzLRA[64]
beefMechanical resistanceLineic impedance index (I); contact impedance (CI)100 HzLRA (I); (CI)[61]
Cull cowsMechanical resistance8 selected parameters related to complex impedance100 Hz–1.5 MHzLRA[59]
Beef and vealTime of postmortem period, , derived from the modified Fricke model40 Hz–110 MHzTA[14]

PSE, DFD, and RFN: pork
Porcine musclepH45Changes in phase angle1 kHzCA[65]
Pig carcassDrip loss at 24 hElectrical impedance1000 HzPLS, RMSEP = 2.53%[66]
Green hampH45, α, and 8 kHz–1 MHzMRA[47]
Lean pork tenderloinsTAC, TVB-N
(, impedance at day 0; , impedance at a given storage interval)
20, 200 Hz, 2, 20, 200 kHzNRA, for TAC;
, for TVB-N
[13]

Freshness: fish
Carp, herring and sea bassHypoxanthine contentPhase angle2.5 kHzLRA for carp; 0.87 for herring; 0.86 for sea bass[67]
Grass carpsTAC, value, TVB-N, sensory assessment (SA)1 kHz, 16 kHzCA for TAC, 0.996 for value, 0.951 for TVB-N, 0.968 for SA[68]
Bighead carppH, texture, TVB-N, value, drop loss, SA and TAC1 kHz, 20 kHzCA for pH and texture,
≥0.955 for TVB-N, value, drop loss, SA and TAC
[69]
Sea breamTVB-NImpedance modulus and phase1 Hz–1 MHzPLS[70]
CarpStorage time (ST), SAMorphological characteristic parameter, impedance modulus, phase1 Hz–1 MHzLRA for ST, 0.66 for SA[17]

Defrosting: fish and chicken
Sea bassSlow-frozen in 1 and 2 cycles, Fast-frozen in 1 and 2 cyclesResistance, reactance, water, fat content, WHC and pH1 Hz–1 MHzPCA, DA78% for total samples, 100% for the unfrozen[71]
SalmonFrozen and stored for 15, 30 and 60 days, frozen in 1 and 2 cyclesImpedance modulus and phase1 Hz–1 MHzPCA, DA71.93%[72]
Sea breamFrozen and stored for 15, 30 and 60 days, frozen in 1 and 2 cyclesImpedance modulus and phase1 Hz–1 MHzPCA, DA70.24%[73]
Sea bassFrozen for 1 month with and without temperature fluctuations (TF), frozen for 4 months with and without TFImpedance modulus and phase0.1–1000 kHzANOVA, LRA between phase angle and protein solubility[74]
Atlantic chub mackerelSlow-frozen in 1 and 2 cycles, Fast-frozen in 1 and 2 cyclesResistance, reactance1 Hz–1 MHzANOVA[75]
Chicken breast meat1 and 2 frozen-thawed cyclesImpedance modulus and phase50–200 kHzLVQNN100% for fresh, >90% for one cycle, >88% for two cycles[60]
Chicken breast meat1, 2, and 3 frozen-thawed cycles,
,
chewiness, hardness, expressible loss
( is the modulus; Ph is the phase angles)
50 Hz, 200 kHzLVQNN100%, 97.5%, 87.5%, and 77.5% for 0, 1, 2, and 3 cycles samples, respectively[15]

Water, fat, and salt: meat
Potted minced pork productsWater (W), lipid (L)Impedance modulus and phase5 kHz–2 MHzPLSA standard deviation of the residues of 0.66% for W and 1.08% for L[76]
Porcine meatWater1–250 kHzLRA, RMSEP = 0.00566,
RSD = 0.73%
[45]
Chicken breast meatWaterImpedance module and phase1 Hz–1 MHzPLS, predictive residual sum of squares of 1.9242[58]
Green hamVisual fatness , α, conformation, ham weight8 kHz–1 MHzMRA[47]
Beef and pork from different size grindsFatResistance, temperature, weight50 kHz, 0.60,0.86 for beef trim,
0.95-, 0.32 cm ground;
, 0.66, 0.92 for pork trim,
0.95,  0.32 cm ground
[77]
Pig of various breeds and cattleIntramuscular fat, resistance at high frequencies immediately after the change of current signal;
, derived for the voltage extrapolated to infinite time
500 Hz–100 kHzSFR, CA for total pork,
for beef
[62]
Minced pork loinSaltImpedance modulus and phase100 Hz–1 MHzPLS[57]
Minced pork loinSodium chloride, sodium nitrite, sodium nitrate contentImpedance modulus and phase1 kHz–1 MHzPLSp1 = 0.985, NR = 0.388 for chloride;
p1 = 0.709, NR = 1.436 for nitrite;
p1 = 0.648, NR = 1.511 for
[78]
Dry-cured hams of three qualities (deep spoilage, spoiled swollen, and unaltered)SaltImpedance modulus and phase100 Hz–1 kHzPLS[79]

Salt and water: fish
Fresh salted Atlantic salmonSalt, water, water phase salt (WPS)Conductance at 1 MHz, capacitance increment 1, 10 MHzLRA for salt,
for water,
for WPS
[80]
Fresh salted rainbow troutSalt, water, WPSImpedance modulus and phase50 kHzLRA, MRA for WPS, 0.844 for water, 0.853 for salt[16]
Salmon during salting-smoking processSalt, water, water activity ()Impedance modulus and phase1 Hz–1 MHzPLSRMSEP = 0.685 for salt, 0.006 for , 3.579 for water[81]
Smoked salmon and cod products (of different brands and batches)Salt, water, lipid, Impedance modulus and phase1 Hz–1 MHzPLS for , for lipid, for water, for salt[82]

Correlation analysis: CA; Linear regression analysis: LRA; Trend analysis: TA; Multiple regression analysis: MRA; Nonlinear regression analysis: NRA; Analysis of variance: ANOVA; Learning vector quantization neural network: LVQNN; Stepwise forward regression: SFR; was not available; parameters of p1 (slope of the fitting line) and norm of residuals (NR) were obtained from the fitting line of predicted and measured salt content, and were used as indicators of performance of the PLS model.