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Journal of Food Quality
Volume 2017, Article ID 8538693, 11 pages
Research Article

Pollen, Physicochemical, and Mineral Analysis of Croatian Acacia Honey Samples: Applicability for Identification of Botanical and Geographical Origin

1University North, 104 Brigade 3, 42000 Varaždin, Croatia
2Catholic University of Croatia, Ilica 242, 10000 Zagreb, Croatia
3Department of Chemistry, Josip Juraj Strossmayer University of Osijek, Kuhačeva 20, 31000 Osijek, Croatia
4Andrija Štampar Teaching Institute of Public Health, Mirogojska 16, 10000 Zagreb, Croatia
5Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, Croatia
6Croatian Environment and Nature Protection Agency, Radnička 80, 10000 Zagreb, Croatia
7Health Centre of the Zagreb County, Ljudevita Gaja 37, 10430 Samobor, Croatia
8Faculty of Medicine, Josip Juraj Strossmayer University of Osijek, Cara Hadrijana 10e, 31000 Osijek, Croatia
9General Hospital Vinkovci, Zvonarska 57, 32000 Vinkovci, Croatia

Correspondence should be addressed to Natalija Uršulin-Trstenjak; rh.ninu@kajnetsrt-nilusru.ajilatan

Received 5 July 2017; Revised 17 October 2017; Accepted 5 December 2017; Published 21 December 2017

Academic Editor: Anca Ioana Nicolau

Copyright © 2017 Natalija Uršulin-Trstenjak et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The aim of the study was to investigate health safety and quality of the Croatian acacia honey, the selected elements in the soil, and whether multivariate methods can provide identification of the origin of honey. The study included 200 acacia honey samples and 100 soil samples from East, Northwest Croatia, and Istria. The proportion of acacia in honey was determined by conducting pollen analysis. Water, free acids, electric conductivity, reducing sugars, saccharose, diastase, and HMF were determined. No significant differences were found using Kruskal-Wallis test regarding the physicochemical parameters (), the mineral content of honey (), or the mineral composition of the soil (). No significant correlation was found between the analyzed elements in honey and soil. Multivariate methods indicated that East Croatia honey samples have higher concentrations of water, HMF, and higher concentrations of measured elements, except for Al. Honey samples from Northwest Croatia are characterized by low concentrations of elements and a higher concentration of saccharose. The Istria samples are richer in reducing sugars, free acids, diastase, higher conductivity, higher content of the acacia pollen grains, and lower concentrations of most metals. Honey from Northwest Croatia and Istria shares the high concentration of Al in honey.